EC Number |
Protein Variants |
Reference |
---|
2.5.1.93 | D208A |
no activity |
702133 |
2.5.1.93 | D211A |
enzyme activity is 1.12% of wild-type activity |
702133 |
2.5.1.93 | D212A |
enzyme activity is 0.11% of wild-type activity |
702133 |
2.5.1.93 | D212A |
site-directed mutagenesis, the mutant shows almost no expression of the recombinant protein |
738205 |
2.5.1.93 | D84A |
enzyme activity is 1.28% of wild-type activity |
702133 |
2.5.1.93 | D87A |
no activity |
702133 |
2.5.1.93 | D87A |
site-directed mutagenesis, the mutant can be be successfully expressed, but is catalytically inactive |
738205 |
2.5.1.93 | D91A |
no activity |
702133 |
2.5.1.93 | K152A |
enzyme activity is 0.15% of wild-type activity |
702133 |
2.5.1.93 | K229A |
enzyme activity is 12.9% of wild-type activity |
702133 |