EC Number |
Protein Variants |
Reference |
---|
2.4.1.232 | D236N |
site-directed mutagenesis, D236 is the first aspartic acid in the canonical GT-A DxD catalytic motif, and mutation to the isosteric asparagine results in the loss of activity |
-, 736926 |
2.4.1.232 | D280N |
site-directed mutagenesis, inactive mutant |
-, 736926 |
2.4.1.232 | H389A |
site-directed mutagenesis, inactive mutant |
-, 736926 |
2.4.1.232 | M16/O16 |
chimeric Mnn9p-Och1p protein, a peptide from the first to the 16th amino acids of Mnn9p is fused with a polypeptide from the 16th to the 480th amino acids of Och1p |
692139 |
2.4.1.232 | M33/O37 |
chimeric Mnn9p-Och1p protein, a peptide from the first to the 33th amino acids of Mnn9p is fused with a polypeptide from the 37th to the 480th amino acids of Och1p |
692139 |
2.4.1.232 | M93/O80 |
chimeric Mnn9p-Och1p protein, a peptide from the first to the 93th amino acids of Mnn9p is fused with a polypeptide from the 80th to the 480th amino acids of Och1p |
692139 |
2.4.1.232 | more |
construction of a yeast DELTAMNN9 enzyme deletion strain, presence of inactive full-length ScMnn9 protein partially rescues the DELTAMNN9 phenotype |
-, 736926 |
2.4.1.232 | more |
DELTAmnn9 mutant with N-glycosylation defect, null mutant, chimera Mnn9 proteins |
650602 |
2.4.1.232 | more |
disruption mutant of och1+: viable, slow-growing phenotype, temperature- and hygromycin B-sensitive, no synthesis of outer chains on acid phosphatase |
651274 |
2.4.1.232 | more |
Mnn9 mutant strain |
-, 636762 |