EC Number   |
Protein Variants |
Reference |
|---|
    2.3.1.37 | A425G/Y428H/R433H/G434N/E435K/L437K |
hexa variant, kcat increased compared to wild-type, Km (Gly) comparable to wild-type, Km (succinyl-CoA) decreased compared to wild-type |
719861 |
| 2.3.1.37 | A425T |
single variant, kcat slightly increased compared to wild-type, Km (Gly) comparable to wild-type, Km (succinyl-CoA) decreased compared to wild-type |
719861 |
| 2.3.1.37 | C145R |
mutant strain G205, lacks enzyme activity, but can complement mutant strain Ole3 with mutation G344C |
486852 |
| 2.3.1.37 | C281P |
the mutant shows about wild type activity. In the presence of 0.001 mM hemin, mutant C281P of isoform HemT retains 100% of its activity |
-, 757686 |
| 2.3.1.37 | C398A |
the mutant shows reduced activity compared to the wild type enzyme |
-, 758425 |
| 2.3.1.37 | D279A |
exchange mutant of potential cofactor binding residue Asp279, no activity, dissociation constant for pyridoxal 5'-phosphate is 19fold increased, different mode of cofactor binding, no formation of quinonoid reaction intermediate, which can be restored by addition of analogue N-methyl-pyridoxal 5'-phosphate |
486856 |
| 2.3.1.37 | D279E |
exchange mutant of potential cofactor binding residue Asp279, 30fold reduced catalytic efficiency for succinyl-CoA compared to the wild-type |
486856 |
| 2.3.1.37 | DELTACT |
the variant lacking residues 535-548 has reduced activity (about 65% of wild type) |
758491 |
| 2.3.1.37 | F557X |
site-directed mutagenesis, a ALAS2 exon 11, c.1670-1671TC>GA mutation |
736819 |
| 2.3.1.37 | G142C |
glycine-rich motif mutant, 15fold increased dissociation constant value for binding of cofactor pyridoxal 5'-phosphate, 6% turnover compared to the wild-type, 4fold increase of Km-value for glycine |
486851 |
