EC Number   |
Protein Variants |
Reference |
|---|
    2.1.3.3 | A240D |
10fold increase of Km at 55°C |
485943 |
| 2.1.3.3 | C273A |
the mutant shows 2.8 and 3.2fold decrease of activity with respect to carbamoyl phosphate and L-ornithine, respectively, compared to the wild type enzyme |
758188 |
| 2.1.3.3 | D140N |
the mutant shows 5.4 and 28fold decrease of activity with respect to carbamoyl phosphate and L-ornithine, respectively, compared to the wild type enzyme |
758188 |
| 2.1.3.3 | D182N |
greatly reduced affinity for L-ornithine, no interaction with arginase |
659213 |
| 2.1.3.3 | D231A |
the mutant shows 450 and 580fold decrease of activity with respect to carbamoyl phosphate and L-ornithine, respectively, compared to the wild type enzyme |
758188 |
| 2.1.3.3 | E105G |
mutant blocked in the active R (relaxed) state |
666766 |
| 2.1.3.3 | E105G |
no cooperativity towards carbamoyl phosphate, follows Michaelis-Menten kinetics |
485937 |
| 2.1.3.3 | E106A |
mutant arcB6240, with strongly reduced cooperativity for carbamoyl phosphate and anabolic activity |
-, 665460 |
| 2.1.3.3 | E106G |
mutant arcB6254, with strongly reduced cooperativity for carbamoyl phosphate and anabolic activity |
-, 665460 |
| 2.1.3.3 | E123A |
little change in activity, or in sensitivity to arginase |
659213 |
