EC Number   |
Protein Variants |
Reference |
|---|
    2.1.1.68 | A162T |
2fold increase in ratio of Vmax to Km-value for caffeic acid compared to wild-type value, 2.9fold decrease in ratio of Vmax to Km-value for 5-hydroxy coniferaldehyde compared to wild-type enzyme, 1.8fold decrease in ratio of Vmax to Km-value for protocatechuic aldehyde compared to wild-type enzyme, 2.8fold decrease in ratio of Vmax to Km-value for 3,4-dihydroxy-5-methoxybenzaldehyde compared to wild-type enzyme, no activity against protocatechuic acid |
660104 |
| 2.1.1.68 | A162T |
same reactivity as wild type |
485531 |
| 2.1.1.68 | A71V |
mutation significantly reduces Klason lignin content and alters lignin composition resulting in a significantly reduced S/G ratio relative to wild-type |
733441 |
| 2.1.1.68 | C296F |
the mutant shows slight activity improvement in O-methylation of N-acetylserotonin compared to the wild type enzyme |
756621 |
| 2.1.1.68 | C296F/Q310L |
the mutant shows about 4.5fold activity improvement in O-methylation of N-acetylserotonin compared to the wild type enzyme |
756621 |
| 2.1.1.68 | C296F/Q310L/V314T |
the mutant shows 9.5fold activity improvement in O-methylation of N-acetylserotonin compared to the wild type enzyme |
756621 |
| 2.1.1.68 | C296H |
the mutant shows about 3fold activity improvement in O-methylation of N-acetylserotonin compared to the wild type enzyme |
756621 |
| 2.1.1.68 | C296H/Q310L |
the mutant shows about 3.5fold activity improvement in O-methylation of N-acetylserotonin compared to the wild type enzyme |
756621 |
| 2.1.1.68 | C296H/Q310L/V314T |
the mutant shows about 6.5fold activity improvement in O-methylation of N-acetylserotonin compared to the wild type enzyme |
756621 |
| 2.1.1.68 | C296Y |
the mutant shows about 2fold activity improvement in O-methylation of N-acetylserotonin compared to the wild type enzyme |
756621 |
