EC Number |
Protein Variants |
Reference |
---|
2.1.1.296 | E145A |
site-directed mutagenesis, the mutant shows strongly affected RNA binding and catalytic activity |
736863 |
2.1.1.296 | H142A |
site-directed mutagenesis, the mutant shows strongly affected RNA binding and catalytic activity |
736863 |
2.1.1.296 | K266A |
site-directed mutagenesis, partly complements the enzyme defective mtant MT57-/- cells |
677099 |
2.1.1.296 | K307A |
site-directed mutagenesis, the mutant shows strongly affected RNA binding and catalytic activity |
736863 |
2.1.1.296 | K74A |
site-directed mutagenesis, the mutant shows strongly affected RNA binding and catalytic activity |
736863 |
2.1.1.296 | L77A |
site-directed mutagenesis, the mutant shows strongly affected RNA binding and catalytic activity |
736863 |
2.1.1.296 | more |
genetic ablation of MT57 is compatible with cell viability and leads to the accumulation of SL RNA with a cap structure defective at positions +3 and +4.. Transsplicing utilization of the SL RNA is not detectably affected in mt57-/- cells, analysis of the SL cap structure in mt57-/- cells, overview |
677099 |
2.1.1.296 | more |
mutants of the K-D-K triad active site residues exchanged for alanine are all cataltyically inactive |
728390 |
2.1.1.296 | more |
silencing of TbMT48 mRNA by RNAi downregulation does not produce a lethal phenotype, as MT48-RNAi trypanosomes remain viable even after 10 days of tetracycline induction, generation of MT48 KO clonal cell lines by homologous recombination with PCR-generated cassettes. An observed defect in cap 4 modification, specific for MT48 ablation, can be complemented by reintroduction of a copy of the MT48 gene into mt48-/- cells |
728268 |
2.1.1.296 | more |
the substitutions of residues S78, H86 and Q113 only mildly affect RNA binding and catalysis, so they are not essential for CMTr2 MTase activity |
736863 |