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Results 1 - 5 of 5
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EC Number Protein Variants Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.232more construction of a multifunctional flavonoid O-methyltransferase fusing tomato 3'-O-methyltransferase OMT3 and Oryza sativa naringenin 7-O-methyltransferase NOMT. The OMT3/NOMT fusion enzyme possesses both 3'- and 7-OMT activities to diverse flavonoid substrates, which are comparable to those of individual OMT3 and NOMT. The OMT3/OsNOMT enzyme also shows 3'- and 7-OMT activity for 7- or 3'-O-methylflavonoids, respectively. The biotransformation of the flavonoids quercetin, luteolin, eriodictyol, and taxifolin using OMT3/NOMT-transformed Escherichia coli generates corresponding di-O-methylflavonoids, rhamnazin, velutin, 3',7-di-O-methyleriodictyol, and 3',7-di-Omethyltaxifolin, respectively 757460
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.232more domain swapping experiments between Nipponbare and Kasalath NOMT genes. The replacement of the Kasalath O-methyltransferase domain with the Nipponbare domain increases the enzyme activity to the level of the Nipponbare enzyme 758005
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.232more production of 7-O-methyl aromadendrin in Escherichia coli strain BL21(DE3) recombinantly expressing the genes of several origins encoding enzymes 4-coumarate–coenzyme A ligase from Petroselinum crispum, chalcone synthase from Petunia hybrida, and chalcone flavanone isomerase from Medicago sativa to produce (2S)-naringenin, and further genes encoding flavanone-3-hydroxylase from Arabidopsis thaliana and the 7-O-methyltransferase from Streptomyces avermitilis to transform (2S)-naringenin into 7-O-methyl aromadendrin via dihydrokaempferol and (2S)-sakuranetin, method and pathway, overview -, 718652
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.232more the recombinant SaOMT is used for 7-O-methylation of naringenin in recombinantly engineered production of 7-O-methyl aromadendrin via sakuranetin from 4-coumaric acid in Escherichia coli, overview -, 718652
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.232T130P mutation in cultivar Kassalth gene to match the corresonding residue in cultivar Nipponbare. Mutant shows activity comparable to Nipponbare NOMT 758005
Results 1 - 5 of 5
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