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EC Number
Protein Variants
Commentary
Reference
1.3.5.2
C130A
loss of activity
390918
1.3.5.2
C130S
loss of activity
390918
1.3.5.2
DELTA1-29
deletion of N-terminal 29 amino acids
657301
1.3.5.2
DELTA1-75
N-terminally truncated enzyme, drastic increase in kcat- and KM-value
655559
1.3.5.2
DELTA2-21
instable in vivo
655723
1.3.5.2
DELTA2-37
instable in vivo
655723
1.3.5.2
DELTA22-37
instable in vivo
655723
1.3.5.2
F115A
mutation slows the rate of flavin reduction by 3 orders of magnitude
696365
1.3.5.2
F188A
considerable changes in the binding affinities of the inhibitors without dramatically affecting the substrate binding affinities
675315
1.3.5.2
F188A
kcat for reaction of L-dihydroorotate + decylubiquinone is 78% of the wild-type value. IC50-value for 2-cyano-3-(9-ethyl-9H-carbazol-3-ylamino)acrylic acid ethyl ester 513fold higher than wild-type value
675315
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