EC Number   |
Protein Variants |
Reference |
|---|
   1.3.3.12 | A113G |
site-directed mutagenesis, in the reaction with 1,4-benzoquinone as electron acceptor, the activity of the dimeric formof A113G is about 1.5fold higher compared to the monomer, the specific activity of the A113G variant in the reaction with molecular oxygen is about 2.5-3times lower than with 1,4-benzoquinone |
-, 724431 |
| 1.3.3.12 | H447G |
the mutant is inactive and shows undetectable FMN binding |
684985 |
| 1.3.3.12 | K450G |
the substitution results in an enzyme that retains an ability to bind FMN (around 50%of the wild type enzyme), but which exhibits no activity |
684985 |
| 1.3.3.12 | K55G |
the mutation has only a minor inhibitory effect on both FMN binding and biochemical activity |
684985 |
| 1.3.3.12 | K55H |
the mutation causes dramatically reduced FMN binding and enzyme activity (above 90%) |
684985 |
| 1.3.3.12 | K55L |
the mutant is inactive and shows undetectable FMN binding |
684985 |
| 1.3.3.12 | W448G |
the mutant is inactive and shows undetectable FMN binding |
684985 |
