EC Number |
Protein Variants |
Reference |
---|
1.14.13.114 | C202A |
site-directed mutagenesis |
-, 764159 |
1.14.13.114 | H211A |
site-directed mutagenesis, the mutant shows moderate uncoupling of their rates of NAD+ and 2,5-DHP product formation, suggesting that the variant has lost some efficiency in hydroxylating the substrate |
-, 764159 |
1.14.13.114 | H302A |
site-directed mutagenesis, the mutant shows moderate uncoupling of their rates of NAD+ and 2,5-DHP product formation, suggesting that the variant has lost some efficiency in hydroxylating the substrate |
764159 |
1.14.13.114 | H47A |
site-directed mutagenesis, inactive mutant, unable to bind FAD |
-, 764159 |
1.14.13.114 | H47E |
site-directed mutagenesis, the mutant can bind FAD, but shows very low activity compared to wild-type. The mutant shows significant consequences in its hydroxylating activity, with NADH oxidization proceeding much more rapidly than 6-HNA is decarboxylated and hydroxylated |
764159 |
1.14.13.114 | H47F |
site-directed mutagenesis, inactive mutant, unable to bind FAD |
-, 764159 |
1.14.13.114 | Y215F |
site-directed mutagenesis, the mutant shows significant consequences in its hydroxylating activity, with NADH oxidization proceeding much more rapidly than 6-HNA is decarboxylated and hydroxylated |
-, 764159 |
1.14.13.114 | Y225F |
site-directed mutagenesis, the mutant shows moderate uncoupling of their rates of NAD+ and 2,5-DHP product formation, suggesting that the variant has lost some efficiency in hydroxylating the substrate |
764159 |