EC Number   |
Protein Variants |
Reference |
|---|
    1.13.11.31 | A410G |
109% of wild-type activity, increase in enantioselectivity |
765361 |
| 1.13.11.31 | A455I |
10% activity of the wild type enzyme |
674491 |
| 1.13.11.31 | A455W |
45% activity of the wild type enzyme |
674491 |
| 1.13.11.31 | C7S |
site-directed mutagenesis, the mutant shows highly reduced iron content compared to the wild-type enzyme, but exchange of the surface-exposed cysteine alters neither the catalytic activity nor the reaction specificity |
688398 |
| 1.13.11.31 | F174A/W176A/L183E/L187E/Y191A |
quintuple mutant disrupts both the hydrophobic and pi-pi interactions of the dimer interface, generating an 12-LOX monomer |
764187 |
| 1.13.11.31 | F390A |
50% activity of the wild type enzyme |
674491 |
| 1.13.11.31 | F390W |
2% activity of the wild type enzyme |
674491 |
| 1.13.11.31 | F424I/I425M |
75% of wild-type activity, 4fold increase in iron content |
765361 |
| 1.13.11.31 | G359F |
catalytically inactive |
765361 |
| 1.13.11.31 | G441A |
the mutant enzyme G441A converts arachidonic acid to (8S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoic acid and (12R)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoic acid in a 1.4:1 ratio, wild-type enzyme converts arachidonate only to (12R)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoic acid |
660365 |
