EC Number |
Protein Variants |
Reference |
---|
1.1.1.45 | D36R |
mutation leads to a switch in the favor of NADP(H) specificity, suggesting an important role of Asp36 in the coenzyme specificity |
669147 |
1.1.1.45 | D36R |
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme |
712765 |
1.1.1.45 | D36R/Q41N |
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme |
712765 |
1.1.1.45 | E157Q |
mutation produces no large kinetic alterations |
669147 |
1.1.1.45 | E97Q |
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme |
712765 |
1.1.1.45 | H145Q |
inactive enzyme form |
669147 |
1.1.1.45 | N195D |
inactive enzyme form |
669147 |
1.1.1.45 | N196Q |
inactive enzyme form |
669147 |
1.1.1.45 | Q41N |
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme |
712765 |
1.1.1.45 | S124A |
mutation decreases the catalytic efficiency 500 fold |
669147 |