EC Number   |
|---|
   2.7.1.78 | - |
| 2.7.1.78 | crystal structure determination and analysis |
| 2.7.1.78 | crystals soaked with DNA oligonucleotides |
| 2.7.1.78 | in complex with the nonhydrolysable ATP analog adenosine 5'-(beta,gamma-imido)triphosphate, sparse matrix sampling, using 100 mM Na2HPO4/KH2PO4 (pH 5.0-6.0), 200 mM NaCl, 15 mM MgCl2, 90 mM sarcosine, and 25% (w/v) polyethylene glycol 1000 |
| 2.7.1.78 | in complexes with GTP, CTP, UTP and dATP, hanging drop vapor diffusion method, using 100 mM sodium citrate, pH 5.6, 100 mM MgCl2, 16-18% (w/v) PEG-3350 |
| 2.7.1.78 | inactivated PNK mutant protein with several 3'-phosphorylated DNAs of different sequence bound in the phosphatase active site |
| 2.7.1.78 | kinase domain of enzyme Pnkp bound to ATP-Mg2+ or ADP-Mg2+, by hanging drop vapor diffusion, protein solution containing 0.6 mM kinase, 2 mM ATP, and 10 mM MgCl2 with an equal volume of precipitant solution containing 100 mM HEPES, pH 7.5, 200 mM MgCl2, and 30% PEG 400, 22°C, 2 days, X-ray diffraction structure determination and analysis at 2.1 A resolution |
| 2.7.1.78 | mutant enzyme D38N in complex with GTP-Mg2+ and a 5'-OH RNA oligonucleotide, hanging drop vapor diffusion method, using 100 mM sodium citrate (pH 5.0), 100 mM MgCl2, 18-24% (w/v) PEG-6000 |
