EC Number   |
|---|
    2.6.1.1 | - |
| 2.6.1.1 | AtPAT wild-type enzyme and K306A, T84V, and T84V/K169V mutant enzymes in complex with either 2-oxoglutarate or pyridoxamine 5'-phosphate and glutamate, X-ray diffraction structure determination and analysis at 1.4-3.0 A resolution, molecular replacement |
| 2.6.1.1 | concentrated isoenzyme AAT-1 solution, 10 mM Tris-HCl, pH 8.0, 1 mM 2-oxoglutarate, 2 mM KOH, 0.01 pyridoxal 5'-phosphate, 0.01% 2-mercaptoethanol, polyethylene glycol 6000 8% w/v, 4°C, 6 days |
| 2.6.1.1 | concentrated isoenzyme AAT-2 solution, 10 mM potassium phosphate, pH 7.0, 1 mM 2-oxoglutarate, 2 mM KOH, 0.001 mM pyridoxal 5'-phosphate, 0.01% 2-mercaptoethanol, dialysis against distilled water at 4°C for 3 days |
| 2.6.1.1 | concentrated protein solution, potassium phosphate 0,01 M, pH 7.2, 0.01 mM pyridoxal 5'-phosphate, ammonium sulfate |
| 2.6.1.1 | crystalized to a maximum resolution of 2.8 A |
| 2.6.1.1 | crystallization of V39F AspAT is performed by the hanging drop vapor diffusion method |
| 2.6.1.1 | crystals are grown at 19°C by the hanging-drop technique, unliganded and hydrocinnamate complex of V39L/K41Y/T47I/N69L/T109S/A293D/N297S, hydrocinnamate complex and maleate complexes of A12T/P13T/N34D/T109S/G261A/S285G/N297S and the hydrocinnamate complex of A12T/P13T/N34D/T109S/G261A/S285G/A293D/N297S |
| 2.6.1.1 | crystals of cytosolic enzyme, or enzyme in complex with substrates, N-methylpyridoxal, and inhibitors, depression-plate and sandwich-box techniques, protein solution: 35-40 mg/ml, pH 5.4, 40 mM sodium acetate, 4% polyethylene glycol 4000-6000, with or without substrate, cofactor or inhibitor compounds, reservoir solution: 40 mM sodium acetate, pH 5.4, 8% polyethylene glycol 4000-6000, crystals appear within 1 week, spectroscopic analysis |
| 2.6.1.1 | crystals of enzyme-inhibitor complex, sitting drop vapour diffusion method, 4°C, protein solution: 7 mg/ml, polyethylene glycol 4000 11% w/v, 50 mM potassium phosphate, pH 7.5, reservoir solution: polyethylene glycol 4000 22% w/v, 50 mM potassium phosphate, pH 7.5, X-ray structure analysis |
