EC Number   |
|---|
   1.1.3.10 | crystallized in hanging drops containing 0.002 ml of a 7 mg/ml protein solution and 0.002 ml of reservoir buffer, 19% PEG-6000 in 100 mM MES, pH 6.0, at 20°C |
| 1.1.3.10 | hanging drop method |
| 1.1.3.10 | hanging drop vapor diffusion method, using 0.1 M MES (pH 5.2), 50 mM MgCl2, and 10% (w/v) monomethylether polyethylene glycol 2000 |
| 1.1.3.10 | hanging drop vapor diffusion method, using 0.1 M Tris-HCl (pH 8.5), 0.1 M MnCl2, and 30% (w/v) polyethylene glycol 400 |
| 1.1.3.10 | mutant enzymes are crystallized by the hanging drop vapor diffusion method, using 10% (w/v) monomethyl ether poly(ethlene glycol) 2000 , 0.1 M MES (pH 5.2), 50 mM MgCl2 |
| 1.1.3.10 | mutant enzymes H167A and T169, Ahanging drop vapor diffusion method, using 0.1 M sodium acetate (pH 4.6), 50 mM MgCl2, and 6% (w/v) monomethyl ether PEG 2000 |
| 1.1.3.10 | P2O H167A mutant in complex with 3-deoxy-3-fluoro-D-glucose, microseeded hanging drops, mixing of equal volumes of 0.1 M MES, pH 5.2, 50 mM MgCl2, 10% w/v PEG monomethyl ether 2000 with 20 mg/ml protein in 20 mM MES, pH 5.2, addition of ligand in saturated mother liquor with 28% w/v PEG 2000, X-ray diffraction structure determination and analysis at 1.35 A resolution |
| 1.1.3.10 | wild-type and mutants, structure analysis, overview |
