EC Number ![](./images/down.png) ![](./images/up.png) |
pH Stability ![](./images/down.png) ![](./images/up.png) |
pH Stability Maximum ![](./images/down.png) ![](./images/up.png) |
Reference ![](./images/down.png) ![](./images/up.png) |
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![Display the word map Display the word map](./images/word_maps.png) 1.1.3.17 | -999 |
- |
at higher pH a transition from alpha-helix to beta-structure occurs while at lower pH the content of alpha-helix structure increases (circular dichroism). Enzyme is more instable at higher pH (melting temperature decreases). At high alkaline pH values the enzyme reveals more accessible hydrophobic patches relative to acidic pH (fluorescence measurement) |
697063 |
![Display the word map Display the word map](./images/word_maps.png) 1.1.3.17 | -999 |
- |
comparison of pka values of the mutants V464A and V464T |
696243 |
![Display the word map Display the word map](./images/word_maps.png) 1.1.3.17 | -999 |
- |
low pH induces a localized and reversible conformational change that is associated with the complete and reversible loss of catalytic activity |
677058 |
![Display the word map Display the word map](./images/word_maps.png) 1.1.3.17 | -999 |
- |
more rapid inactivation at alkaline than at acidic pH at both 27°C and 37°C. pH-dependent secondary structure changes are analysed |
695307 |
![Display the word map Display the word map](./images/word_maps.png) 1.1.3.17 | -999 |
- |
prolonged incubation of the inactive enzyme at pH 6 and temperatures above 20°C reveals slow and full recovery of activity over 3 hours, linked to conformational change reverting the enzyme to the native form, rate of approaching steady state independent of concentrations of choline and enzyme, increased to a limiting value with increasing pH |
689966 |
![Display the word map Display the word map](./images/word_maps.png) 1.1.3.17 | 3 |
6 |
inactivation takes place at both 27°C and 37°C |
695307 |
![Display the word map Display the word map](./images/word_maps.png) 1.1.3.17 | 6 |
9 |
inactivation takes place at both 27°C and 37°C |
695307 |
![Display the word map Display the word map](./images/word_maps.png) 1.1.3.17 | 7 |
8.5 |
no significant inactivation at both 27°C and 37°C |
695307 |
![Display the word map Display the word map](./images/word_maps.png) 1.1.3.17 | 7 |
9 |
50% initial activity is lost at 40°C at pH 6.0 and 9.5 |
389719 |
![Display the word map Display the word map](./images/word_maps.png) 1.1.3.17 | 8 |
10.2 |
purified enzyme, 30°C, 10 min, stable at |
724598 |