EC Number   |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
|---|
   2.3.1.5 | -999 |
- |
incubation at 42°C for 10 min: 30% remaining activity isoenzyme NAT-a |
687848 |
| 2.3.1.5 | -999 |
- |
incubation at 42°C for 10 min: 50% remaining activity isoenzyme Nat-b |
687848 |
| 2.3.1.5 | -999 |
- |
no refolding after thermal denaturation |
657884 |
| 2.3.1.5 | 25 |
- |
the enzyme is stable at room temperature for more than 24 hours, 34% of enzyme activity is retained after 21 days at 25°C |
737268 |
| 2.3.1.5 | 34 |
- |
inactivation above |
487119 |
| 2.3.1.5 | 37 |
- |
half life of 32 min |
487143 |
| 2.3.1.5 | 37 |
- |
t1/2: 35 h NAT2 wild-type, 3.5 h Cys44Gly-mutant, 0.5 h Cys223Gly-mutant |
487131 |
| 2.3.1.5 | 37 |
47 |
NAT1 is sensitive to heat denaturation and shows a significant loss in activity as temperature is increased from 37°C to 47°C |
736009 |
| 2.3.1.5 | 40 |
47 |
NAT enzymes unfold cooperatively with concomitant loss of activity at 40-47°C |
686618 |
| 2.3.1.5 | 50 |
- |
recombinant isozyme Nat1 is completely stable for 120 min, recombinant isozyme Nat3 loses most activity within 80 min, recombinant isozyme Nat2 is almost inactivated within 5 min |
675599 |
