EC Number   |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
|---|
    2.3.1.37 | -999 |
- |
- |
486820, 486823, 486851 |
| 2.3.1.37 | -999 |
- |
comparison of thermostability of wild-type and mutant ALAS2 aApoenzymes, overview |
736819 |
| 2.3.1.37 | -999 |
- |
room temperature, inactivation within 30 min |
486809 |
| 2.3.1.37 | -999 |
- |
t1/2 is 48.6°C, 52.5°C, 49.9°C, and 51.4°C for wild-type hALAS2, delAT, delAGTG, and Q548X, respectively |
735684 |
| 2.3.1.37 | -999 |
- |
temperature insensitive |
486820 |
| 2.3.1.37 | -999 |
- |
the secondary structure of the enzyme is mostly resilient to temperature changes, overview |
735711 |
| 2.3.1.37 | -999 |
- |
thermal stability of glycine-rich motif mutants is reduced compared to the wild-type |
486851 |
| 2.3.1.37 | 37 |
- |
60 min, 20% loss of activity with addition of 2-mercaptoethanol and glycerol, 60% loss of activity without addition of 2-mercaptoethanol and glycerol |
486811 |
| 2.3.1.37 | 37 |
- |
after 60 min incubation at 37°C, the residual activity of wild type dramatically drops to 8% of the original activity |
756270 |
| 2.3.1.37 | 45 |
- |
t1/2 of the wild-type is 11.7 min, and t1/2 of mutant enzymes are between 3.8 and 12.8 min |
736819 |
