EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
---|
1.1.3.17 | -999 |
- |
freezing at low pH leads to reversible conformational change, associated with complete and reversible loss of catalytic activity |
689966 |
1.1.3.17 | -999 |
- |
temperature dependence of rate constants of the E312D mutant is analysed, substrate choline |
695885 |
1.1.3.17 | 30 |
- |
wild-type, stable for at least 3 h |
762910 |
1.1.3.17 | 32 |
- |
melting temperature of wild-type |
762910 |
1.1.3.17 | 40 |
- |
mutant S101A/V355T/F357R/M359R, 3 h, 10% residual activity. Mutant S101A/D250G/F253R/V355T/F357R/M359R, 3 h, almost 50% residual activity |
762910 |
1.1.3.17 | 40 |
45 |
incubated for 10 min at pH 8.5 and 40°C 20% initial activity is lost, 96% at 45°C, only 10% initial activity is lost at 45°C in presence of 10% glycerol |
389719 |
1.1.3.17 | 52 |
- |
metling temperature of mutant S101A/D250G/F253R/V355T/F357R/M359R |
762910 |
1.1.3.17 | 70 |
90 |
no degradation when incubated for 1 h |
698402 |