EC Number   |
|---|
    2.6.1.1 | - |
| 2.6.1.1 | AAT1, AAT2, and AAT5, overexpression in Escherichia coli strains |
| 2.6.1.1 | aspartate aminotransferase construct consists of the Arabidopsis thaliana ASP5 coding region followed by mGFP5. This construct is designated SP5:GFP. GFP is fused to the carboxy-terminus of ASP5 to preserve the cleavage site between the ASP5 transit peptide and mature protein, thus ensuring proper plastid import and processing. A five amino acid linker peptide (GSGGG) is inserted between ASP5 and GFP to promote proper folding and activity of both proteins. The constructs are introduced into Nicotiana tabacum cv. Samsun NN via Agrobacterium-mediated transformation of leaf strips. ASP5:GFP is present in stromules of Nicotiana tabacum. Arabidopsis thaliana ASP5, with GFP fused to its carboxy-terminus, can interact with itself and with the Nicotiana tabacum AAT3 to form enzymes that are functional in vitro |
| 2.6.1.1 | cloning and DNA-sequencing of cDNA encoding the plastidic isozyme pAspAT, DNA and amino acid sequence analysis |
| 2.6.1.1 | cloning of a liver cDNA fragment, nucleotides 1-419, into a plasmid for production of cRNA probes |
| 2.6.1.1 | cloning, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli AB1157 |
| 2.6.1.1 | expressed in Escherichia coli |
| 2.6.1.1 | expressed in Escherichia coli as a His-tagged fusion protein |
| 2.6.1.1 | expressed in Escherichia coli BL21 (DE3) cells |
| 2.6.1.1 | expressed in Escherichia coli BL21 CodonPlus (DE3)-RIL cells |
