EC Number   |
|---|
    2.5.1.22 | - |
| 2.5.1.22 | DNA fragment encoding human enzyme is amplified by PCR and subcloned into the pET28a-LIC vector downstream of the polyhistidine coding region. Enzyme is expressed in the Escherichia coli BL21-Codon Plus(DE3)-RIL strain by the addition of 1 mM isopropyl1-thio-beta-D-galactopyranoside. The N-terminally truncated SpmSyn mutants are generated by PCR and subcloned into the pET28a-LIC vector. |
| 2.5.1.22 | DNA sequence determination and analysis of gene SPMS, construction of expression vectors for recombinant expression in transgenic plants/mutants of genes ACAULIS5, i.e. ACL5, and SPMS |
| 2.5.1.22 | enzyme overexpression in transgenic mice under control of a composite CMV-IE enhancer-chicken beta-actin promotor, 4 separate founder CAG/SpmS mice are analysed: enzyme expression in all tissues, mostly highly increased compared to the wild-type mice, overview |
| 2.5.1.22 | expressed in a yeast mutant deficient in Spm synthase, and in the Arabidopsis dwarf mutant acl5 |
| 2.5.1.22 | expressed in Escherichia coli |
| 2.5.1.22 | expressed in Escherichia coli BL21(DE3) cells |
| 2.5.1.22 | expressed in Escherichia coli XL-1 Blue cells |
| 2.5.1.22 | expressed in Nicotiana tabacum leaves |
| 2.5.1.22 | expression of human spermine synthase in CAGSMS line 8 mice from a composite CMV-IE enhancer/chicken beta-actin promoter. Transgenic expression of spermine synthase in the Gy mice reverse all of the increases in dcAdoMet content and AdoMetDC |
