EC Number   |
|---|
    2.5.1.18 | - |
| 2.5.1.18 | 79 genes encoding GSTs, DNA and amino acid sequence determination and analysis, sequence compariosns and phylogenetic analysis, detailed overview |
| 2.5.1.18 | a synthetic GST gene based on the wild type amino acid sequence, changing the second residue to an isoleucine, is overexpressed in Escherichia coli strain BL21 to obtain GST as a soluble protein |
| 2.5.1.18 | co-expression of HA-tagged GST P1 with Flag-tagged DELTAMEKK1 in HEK-293 cells, the overexpression of isozyme GST P1 leads to inhibition of both DELTAMEKK1- and etoposide-induced apoptosis, and inhibition of procaspase-3 activation and PARP cleavage, overview |
| 2.5.1.18 | constitutive gst gene, expression of the enzyme as fusion protein with the full-length intracellular domain of the human insulin receptor, i.e. GST-IRK, comprising the coding region of IRK, residues Val966Lys1271, C969S, Y972F, and containing a thrombin-cleavage site as a linker between GSTand IRK, in Spodoptera frugiperda Sf9 cells |
| 2.5.1.18 | construction of large libraries of isozyme GST T1-1 constructed by error prone PCR, DNA shuffling, saturation mutagenesis, or a random mutagenesis in conjunction with homologous recombination, screening for improved catalytic activity towards CMAC in a quantitative fashion using flow cytometry, up to a 20000fold increase in kcat/KM compared to wild-type GSTT1-1 is achieved, residue Trp234 is important, overview, recombinant expression of wild-type and mutants as His6-tagged enzymes |
| 2.5.1.18 | correlation of expression of GST family members with alterations in the transcription of other genes, expression patterns, overview |
| 2.5.1.18 | Der p 8, DNA and amino acid sequence determination and analysis, expression of Der p 8 in Pichia pastoris srain GS115 |
| 2.5.1.18 | DNA and amino acid sequence determination and analysis, expression analysis |
| 2.5.1.18 | DNA and amino acid sequence determination and analysis, expression of the His-tagged enzyme in Escherichia coli strain DH5alpha |
