EC Number   |
|---|
    2.3.1.21 | - |
| 2.3.1.21 | 2 isoforms of CPT I, liver L-CPT I, skeletal muscle M-CPT I, cloned from liver, expression in Pichia pastoris GS115, DNA sequence determination |
| 2.3.1.21 | a rat promoter CPT-Ialpha luciferase vector is constructed |
| 2.3.1.21 | adenovirus-mediated (over)expression in cultured fed rat hepatocyte |
| 2.3.1.21 | clonig of CPT I and II from liver, expression in Pichia pastoris |
| 2.3.1.21 | cloning and expression of CPT II in Escherichia coli, overexpression of CPT II in Spodoptera frugiperda Sf 9 cells via baculavirus infection, DNA sequence analysis |
| 2.3.1.21 | cloning of liver mitochondrial CPT II from genetic library, in vitro transcription and translation, DNA sequence analysis, expression in COS cells |
| 2.3.1.21 | construction of 6 chimeric proteins with exchanges structure segments of L-CPT I and M-CPT I, expression of chimeric mutants and wild-type isoforms in Pichia pastoris |
| 2.3.1.21 | construction of chimeric L-CPT I with deletions and exchanged C-terminal sequences between rat and pig enzymes, expression in Pichia pastoris |
| 2.3.1.21 | COS-1 cells are transiently transfected to express a fusion protein in which enhanced green fluorescent protein is fused to the C-terminus of L-CPT1. This fusion protein is localized to mitochondria, and possibly to peroxisomes, but not to the endoplasmic reticulum |
