EC Number   |
|---|
    1.5.1.20 | - |
| 1.5.1.20 | cloning, disruption and expression of the two genes MET12 and MET13 encoding isoenzymes of MTHFR, MET12 is located on chromosome XVI and encodes a 657 amino acids protein, MET13 is located on chromosome VII, encodes a 599 amino acids protein and is responsible for most of the activity in cell, expression in Escherichia coli BL21 |
| 1.5.1.20 | enzyme is encoded by the 2 genes met9 and met11, which can complement the double-mutant strain, but they cannot complement for each other in the met9-deficient and met11-mutant, respectively, DNA and amino acid sequence determination and analysis |
| 1.5.1.20 | expressed in Epstein-Barr virus-transformed lymphoblasts |
| 1.5.1.20 | expressed in Escherichia coli and Neuro-2a cells |
| 1.5.1.20 | expressed in Escherichia coli BL21(DE3) cells |
| 1.5.1.20 | expressed in HepG2 cells |
| 1.5.1.20 | expressed in Mus musculus |
| 1.5.1.20 | expression of chimeric mutant in an enzyme-deficient strain, overexpression of the plant enzyme from Arabidopsis thaliana in an enzyme-deficient strain can complement the mutant and results in 8fold increased accumulation of S-adenosyl-L-methionine, mechanism overview |
| 1.5.1.20 | expression of recombinant human MTHFR at high levels in Sf9 cells from Spodoptera frugiperda by using a baculovirus expression system |
