EC Number   |
|---|
    1.4.1.1 | - |
| 1.4.1.1 | AlaDH gene is cloned into a pGEX6p-1 and expressed in Escherichia coli JM109. AlaDH gene is then cloned intoa vector pNZ8148 to generate a vector pNZ8148/ald. The same AlaDH gene is placed downstream of the lactate dehydrogenase (ldh) promoter from Streptococcus thermophilus to generate pNZ273/ldhp/AlaDH. The pNZ8148/AlaDH and pNZ273/ldhp/AlaDH are introduced separately in Lactococcus lactis NZ9000. As a result of over-expressed AlaDH, the production of alanine detected by HPLC in Lactococcus lactis NZ9000 carrying pNZ273/ldhp/ald reaches 52 mg/ml, an approximately 26fold increase compared to the parent strain Lactococcus lactis NZ9000, but not in Lactococcus lactis NZ9000 carrying pNZ8148/ALaDH |
| 1.4.1.1 | cloned and expressed in Escherichia coli |
| 1.4.1.1 | expressed in Escherichia coli BL21(DE3) cells |
| 1.4.1.1 | expressed in Escherichia coli strain TBLA-1, an H+-ATPase- and lactate dehydrogenase-defective mutant of Escherichia coli derived from the pyruvate producing K-12 strain W1485lip2 |
| 1.4.1.1 | expressed in Escherichia coli strain W (ATCC 9637) |
| 1.4.1.1 | expression in Escherichia coli |
| 1.4.1.1 | expression in Escherichia coli JM109 |
| 1.4.1.1 | expression in Escherichia coli TG1 with a vector plasmid, pUC118 |
| 1.4.1.1 | expression of N-terminally GFP-fused enzyme in Anabaena sp. strain CSR30 |
