EC Number |
---|
1.11.1.18 | - |
1.11.1.18 | enzyme is overproduced in Streptomyces lividans TK64, up to 30000 times compared to Streptomyces aureofaciens |
1.11.1.18 | expressed in Escherichia coli BL21(DE3) cells |
1.11.1.18 | expression in Escherichia coli |
1.11.1.18 | expression in Escherichia coli. Recombinant enzyme and its mutants have no enzyme activities, unless they are activated by Na3VO4 |
1.11.1.18 | expression in Saccharomyces cerevisiae |
1.11.1.18 | gene bpo-A1, recombiant expression of His-tagged enzyme in Escherichia coli strain Rosetta 2 (DE3) |
1.11.1.18 | gene phyA, DNA and amino acid sequence determination and analysis, recombinant expression of the codon-optimized phytase gene in Pichia pastoris strain X-33, quantitative real-time PCR for gene copy number determination |
1.11.1.18 | overexpressed in Escherichia coli. The enzyme is found to be predominantly in the form of inclusion bodies |
1.11.1.18 | the gene is cloned in the positive selection vector pIJ699 and expression in Streptomyces lividans TK64. The cloned bromoperoxidase is overproduced up to 2800fold by the Streptomyces lividans transformant |