EC Number   |
|---|
    1.1.1.184 | - |
| 1.1.1.184 | 3 forms from rat testis |
| 1.1.1.184 | CBR1 coding regions excised from a pOTB7 recombinant plasmid and subcloned into pET28a vector. Constructs gave rise to a human CBR1 enzyme with a 6x-His tag separated by an 18-amino acid residue linker on the amino terminus. A factor Xa site inserted at the amino terminus between the linker and CBR1 gene. The pET28a constructs of the CBR1 wild-type and variant enzymes heat-shock transformed into Escherichia coli BL21 (DE3) pLysS-competent cells and expressed under the control of an IPTG-inducible T7 polymerase |
| 1.1.1.184 | CBR1 expressed as histidine-tagged protein |
| 1.1.1.184 | CBR3 expressed as histidine-tagged protein |
| 1.1.1.184 | co-expressed with glucose dehydrogenase from Exiguobacterium sibiricum in Escherichia coli BL21 (DE3) cells |
| 1.1.1.184 | co-expressed with glucose dehydrogenase from Thermus acidophilum in Escherichia coli BL21(DE3) cells |
| 1.1.1.184 | DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) |
| 1.1.1.184 | expressed from vector pET-22b in Escherichia coli BL21 (DE3) |
| 1.1.1.184 | expressed in Escherichia coli BL21 cells |
