EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
2.4.1.B46 | dTDP-2-deoxy-alpha-L-fucose + 4-O-[(4-O-2-deoxy-alpha-L-fucosyl)-alpha-L-rhodosaminyl]aklavinone |
AknK also catalyzes a tandem addition of a second L-2-deoxyfucosyl moiety, albeit with reduced activity, to the natural disaccharide chain to produce L-deoxyfucosyl-L-deoxyfucosyl-L-rhodosaminyl aklavinone, a variant of the natural aclacinomycin A. The second transfer reaction is 3 orders of magnitude slower than the first sugar transfer reaction catalyzed by AknK |
Streptomyces galilaeus |
dTDP + 4-O-[[4-O-2-deoxy-alpha-L-fucosyl-(4-O-alpha-L-2-deoxyfucosyl)]alpha-L-rhodosaminyl]aklavinone |
- |
? |
2.4.1.B46 | dTDP-2-deoxy-alpha-L-fucose + 4-O-[(4-O-2-deoxy-alpha-L-fucosyl)-alpha-L-rhodosaminyl]aklavinone |
- |
Streptomyces galilaeus |
dTDP + 4-O-[[4-O-alpha-L-2-deoxyfucosyl-(4-O-2-deoxy-alpha-L-fucosyl)]alpha-L-rhodosaminyl]aklavinone |
- |
? |
2.4.1.B46 | dTDP-2-deoxy-alpha-L-fucose + idarubicin |
- |
Streptomyces galilaeus |
dTDP + 4'-O-(2-deoxy-alpha-L-fucosyl)idarubicin |
- |
? |
2.4.1.B46 | dTDP-2-deoxy-beta-L-fucose + 4-O-(alpha-L-rhodosaminyl)aklavinone |
- |
Streptomyces galilaeus |
dTDP + 4-O-[(4-O-2-deoxy-alpha-L-fucosyl)-alpha-L-rhodosaminyl]aklavinone |
- |
? |
2.4.1.B46 | dTDP-L-daunosamine + 4-O-(alpha-L-rhodosaminyl)aklavinone |
the enzyme accepts L-daunosamine as an alternative sugar substrate, albeit with a 270fold decrease in catalytic efficiency (kcat/Km) |
Streptomyces galilaeus |
dTDP + 4-O-[(4-O-alpha-L-daunosaminyl)-alpha-L-rhodosaminyl]aklavinone |
- |
? |
2.4.1.B46 | more |
AknK also accepts an alternate dTDP-L-sugar, dTDP-L-daunosamine, and other monoglycosylated anthracyclines, including daunomycin, adriamycin, and idarubicin, to build alternate disaccharides on variant anthracycline backbones |
Streptomyces galilaeus |
? |
- |
? |
2.4.1.B46 | more |
the enzyme does not transfer TDP-L-rhodosamine |
Streptomyces galilaeus |
? |
- |
? |