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Search term: culture condition

Results 1 - 44 of 44
EC Number Recommended Name Source Tissue Commentary Reference
Show all pathways known for 1.1.1.1Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.1alcohol dehydrogenase culture condition adhA transcription is induced by ethanol or n-propanol, adhA transcription is subject to glucose catabolite repression. Accordingly, both induction of AdhA activity and ethanol utilization are detected only after depletion of glucose 684612
Show all pathways known for 1.1.1.337Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.337L-2-hydroxycarboxylate dehydrogenase (NAD+) culture condition maximum specific and volume activities are found at the end of the exponential growth phase, during further fermentation enzyme activity declines rapidly and is no longer detectable after 15 h 726750
Show all pathways known for 1.1.1.368Display the reaction diagram Show all sequences 1.1.1.3686-hydroxycyclohex-1-ene-1-carbonyl-CoA dehydrogenase culture condition when cells are grown aerobically with benzoate the activity is 20-25fold lower than in cells grown anaerobically with benzoate 724904
Show all pathways known for 1.1.1.49Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.49glucose-6-phosphate dehydrogenase (NADP+) culture condition KlZWF1 is constitutively expressed. Its deletion leads to increased sensitivity to hydrogen peroxide on glucose. The Klzwf1DELTA strain has a reduced biomass yield on fermentative carbon sources as well as on lactate and glycerol 686466
Show all pathways known for 1.1.1.49Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.49glucose-6-phosphate dehydrogenase (NADP+) culture condition the highest glucose 6-phosphate dehydrogenase activity occurrs when the glucose solution is fed into the fermenter through the decreasing linear mode 684518
Show all pathways known for 1.1.1.51Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.513(or 17)beta-hydroxysteroid dehydrogenase culture condition exposure of the fish to water-soluble fraction of crude oil produces an apparent concentration-specific increase of 3beta-hydroxysteroid dehydrogenase 686428
Show all pathways known for 1.1.3.13Display the word mapDisplay the reaction diagram Show all sequences 1.1.3.13alcohol oxidase culture condition when incubated in the cholesterol medium, the alcohol oxidase activity reaches the maximum at 2 days of cultivation, and then gradually decreases. Methanol, ethanol and isopropanol are also effective for production of alcohol oxidase, but glycerol, glucose and malic acid are not -, 688123
Display the word mapDisplay the reaction diagram Show all sequences 1.1.3.6cholesterol oxidase culture condition the strain reaches its maximal utilization of cholesterol as the only C source for production of extracellular cholesterol oxidase 684242
Display the word mapDisplay the reaction diagram Show all sequences 1.1.3.9galactose oxidase culture condition maximum galactose oxidase production (approximately 4.0 U/ml) is obtained when fermentation is carried out at 25°C, with orbital shaking (100 rpm) and an initial medium of pH 7.0, for 96 h, using a 2% (v/v) inoculum made from a homogenized four-day-old liquid culture, in the presence of copper, manganese, and magnesium 687434
Display the word mapDisplay the reaction diagram Show all sequences 1.10.3.2laccase culture condition fungal cultures grown in malt extract medium -, 684626
Display the word mapDisplay the reaction diagram Show all sequences 1.10.3.2laccase culture condition malt extract is the carbon source which yields the highest laccase production, with a maximum of laccase production reached after 9 days of cultivation. The nitrogen source soya peptone yields the highest laccase production 687338
Display the word mapDisplay the reaction diagram Show all sequences 1.11.1.13manganese peroxidase culture condition grown on banana waste -, 765837
Display the word mapDisplay the reaction diagram Show all sequences 1.11.1.13manganese peroxidase culture condition grown on bark mulch and wood chips 765837
Display the word mapDisplay the reaction diagram Show all sequences 1.11.1.13manganese peroxidase culture condition grown on dyes containing agar plates -, 765837
Display the word mapDisplay the reaction diagram Show all sequences 1.11.1.13manganese peroxidase culture condition grown on GPYM agar plates 765837
Display the word mapDisplay the reaction diagram Show all sequences 1.11.1.13manganese peroxidase culture condition grown on Japanese beech and cedar wood, Eucalyptus grandis wood, and Bamboo culms 765837
Display the word mapDisplay the reaction diagram Show all sequences 1.11.1.13manganese peroxidase culture condition grown on MEG agar slant -, 765837
Display the word mapDisplay the reaction diagram Show all sequences 1.11.1.13manganese peroxidase culture condition grown on mineral salt media 765837
Display the word mapDisplay the reaction diagram Show all sequences 1.11.1.13manganese peroxidase culture condition grown on potato dextrose agar -, 765837
Display the word mapDisplay the reaction diagram Show all sequences 1.11.1.13manganese peroxidase culture condition grown on potato dextrose broth (PDB) 765837
Display the word mapDisplay the reaction diagram Show all sequences 1.11.1.13manganese peroxidase culture condition grown on solid-state fermentation medium -, 765837
Display the word mapDisplay the reaction diagram Show all sequences 1.11.1.13manganese peroxidase culture condition grown on wheat straw, rice straw, agriculture byproducts, or agro-industrial wastes 765837
Display the word mapDisplay the reaction diagram Show all sequences 1.11.1.13manganese peroxidase culture condition strain Al-Dhabi 140 is grown in mineral salt medium containing 25, 50, 75, 100, 125, 150, 175 and 200 mg/l of tetracycline with 7% inoculum under shaking condition at 150 rpm.Tetracycline concentration in the culture medium is assayed after eight days. The degradation potential by strain Al-Dhabi 140 is 71 mg/l and 75.3 mg/l when the tetracycline concentration is 125 and 150 mg/l, respectively -, 764508
Show all pathways known for 1.13.11.1Display the word mapDisplay the reaction diagram Show all sequences 1.13.11.1catechol 1,2-dioxygenase culture condition monocyclic hydrocarbons, phenol, catechol, benzoic acid and vanillic acid -, 702976
Show all pathways known for 1.13.11.2Display the word mapDisplay the reaction diagram Show all sequences 1.13.11.2catechol 2,3-dioxygenase culture condition monocyclic hydrocarbons, phenol, catechol, benzoic acid, protocatechuic acid and vanillic acid -, 702976
Show all pathways known for 1.13.11.3Display the word mapDisplay the reaction diagram Show all sequences 1.13.11.3protocatechuate 3,4-dioxygenase culture condition monocyclic hydrocarbons, 4-hydroxybenzoic acid, protocatechuic acid and vanillic acid -, 702976
Show all pathways known for 1.3.1.33Display the word mapDisplay the reaction diagram Show all sequences 1.3.1.33protochlorophyllide reductase culture condition low expression, light-response during greening, expression in mature cells 656908
Show all pathways known for 2.4.1.49Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.49cellodextrin phosphorylase culture condition cells grown in continuous culture under cellobiose or cellulose limitation over a 10fold range of dilution rates. The gene displays modest difference in expression with growth rate or substrate type 671420
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.132chitosanase culture condition medium containing shrimp heads as the sole carbon and nitrogen source -, 751450
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.132chitosanase culture condition the highest level of enzyme activity (186 U/ml) is achieved in culture medium using high cell-density cultivation in a 7-l fermenter. Chitosanase is successfully secreted to the culture media through the widely used SecB-dependent type II pathway in Escherichia coli 749607
Display the word mapDisplay the reaction diagram Show all sequences 3.4.19.13glutathione gamma-glutamate hydrolase culture condition fermentation broth of carbon stressed cultures -, 754814
Show all pathways known for 3.5.1.1Display the word mapDisplay the reaction diagram Show all sequences 3.5.1.1asparaginase culture condition carbon sources such as sucrose, maltose, galactose, lactose, mannitol and mannose inhibit enzyme production. Exogenous cAMP in presence of carbon sources stimulates L-asparaginase enzyme production 686239
Show all pathways known for 3.5.1.1Display the word mapDisplay the reaction diagram Show all sequences 3.5.1.1asparaginase culture condition high L-asparaginase activity is found in cells cultured on L-fructose, D-galactose, sucrose or maltose, and in cells cultured on L-asparagine as the sole nitrogen source 686239
Show all pathways known for 3.5.1.1Display the word mapDisplay the reaction diagram Show all sequences 3.5.1.1asparaginase culture condition L-asparaginase-I is constitutive 686239
Show all pathways known for 3.5.1.1Display the word mapDisplay the reaction diagram Show all sequences 3.5.1.1asparaginase culture condition L-asparaginase-II is secreted in response to N starvation 686239
Show all pathways known for 3.5.1.1Display the word mapDisplay the reaction diagram Show all sequences 3.5.1.1asparaginase culture condition sabourand dextrose broth yields maximum growth and maximum L-asparaginase production 686239
Display the word mapDisplay the reaction diagram Show all sequences 3.5.1.11penicillin amidase culture condition 51 g/l of casein hydrolyzed with Alcalase and 2.7 g/l of phenylacetic acid (PhAc), the following carbon substrates are tested, individually and combined: glucose, glycerol, and lactose (present in cheese whey). Glycerol and glucose are effective nutrients for the microorganism growth but delay the penicillin G acylase production. Cheese whey always increases enzyme production and cell mass. However lactose (present in cheese whey) is not a significant carbon source for Bacillus megaterium. Phenylacetic acid, amino acids, and small peptides present in the hydrolyzed casein are the actual carbon sources for enzyme production. Replacement of hydrolyzed casein by free amino acids, 10.0 g/l, leads to a significant increase in enzyme production (approximately 150%), with a preferential consumption of alanine, aspartic acid, glycine, serine, arginine, threonine, lysine, and glutamic acid. A decrease of the enzyme production is observed when 20.0 g/l of amino acids are used. Using the single omission technique, it is shown that none of the 18 tested amino acids is essential for enzyme production. The use of a medium containing eight of the preferentially consumed amino acids leads to similar enzyme production level obtained when using 18 amino acids. Phenylacetic acid, up to 2.7 g/l, does not inhibit enzyme production, even if added at the beginning of the cultivation 685709
Show all pathways known for 3.5.1.2Display the word mapDisplay the reaction diagram Show all sequences 3.5.1.2glutaminase culture condition optimizing concentration of sucrose, yeast extract, glutamine, and sodium chloride for L-glutaminase production by response surface methodology 685637
Show all pathways known for 3.5.1.23Display the word mapDisplay the reaction diagram Show all sequences 3.5.1.23ceramidase culture condition when the strain is cultured with sphingomyelin or phosphatidylcholine, production of the enzyme drastically increases, causing the increase of hemolytic activity in the cellfree culture supernatant. Ceramide and sphingosine are effective in promoting the production of ceramidase -, 680936
Display the word mapDisplay the reaction diagram Show all sequences 3.5.1.41chitin deacetylase culture condition chitin as sole carbon resource of culture medium. The enzyme activity is 10-11 units/ml culture supernatant after the strain is shaken at 200 rpm and 29°C for 96 h 685962
Show all pathways known for 4.2.1.84Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.84nitrile hydratase culture condition optimum growth temperature of the bacterium is 25°C to 30°C, but it is able to grow at 4°C 729043
Display the word mapDisplay the reaction diagram Show all sequences 4.3.1.B2imidazole glycerol phosphate synthase culture condition the production of the soluble HIS7 is highest at 25°C with 1 mM isopropyl beta-D-thiogalactopyranoside and this condition is used for the large-scale protein preparations 728731
Display the word mapDisplay the reaction diagram Show all sequences 5.6.2.2DNA topoisomerase (ATP-hydrolysing) culture condition actively dividing Sulfolobus solfataricus cells contain only small amounts of both reverse gyrases, approximately 50 TopR1 and 125 TopR2 molecules per cell at 80°C. Sulfolobus solfataricus cells are resistant at 45°C for several weeks, but there is neither cell division nor replication initiation; these processes are fully restored upon a return to 80°C. TopR1 is not found after three weeks at 45°C whereas the amount of TopR2 remains constant -, 729504
Show all pathways known for 6.2.1.40Display the word mapDisplay the reaction diagram Show all sequences 6.2.1.404-hydroxybutyrate-CoA ligase (AMP-forming) culture condition cells grown under strict H2-CO2 autotrophy -, 725542
Results 1 - 44 of 44