EC Number   |
Reference |
|---|
    2.5.1.18 | in 0.1 M potassium phosphate buffer (pH 6.5) containing 1 mM EDTA and 5 mM dithiothreitol, with 4 M guanidine hydrochloride |
689948 |
| 2.5.1.18 | in 6 M urea containing reduced/oxidized glutathione refolding solution |
686209 |
| 2.5.1.18 | low guanidine-HCl concentrations, less than 0.5 M, denature the enzyme, complete renaturation is possible. At higher concentrations of the denaturant, 0.5-4 M, complete renaturation is not achieved. The extent of renaturation is a function of protein concentration |
662074 |
| 2.5.1.18 | partial regeneration after urea and guanidine hydrochloride denaturation |
637939 |
| 2.5.1.18 | the denaturation process is fully reversible at pH 7.5 |
685020 |
| 2.5.1.18 | wild-type enzyme becomes completely unfolded after incubation for 10 min in 4 M guanidinium chloride. Refolding rate of wild-type enzyme is 0.54 per min, 20.7% recovery |
661016 |
| 2.5.1.18 | wild-type enzyme shows 25.42% of the initial activity after denaturation and refolding, mutant K40A 20.51%, mutant I54A 8.51%, mutant E66A 51.68% and mutant S67A 34.33% |
661756 |
