2.1.2.1 | 5 ml of supernatant containing the solubilized domain is added drop by drop to 100 ml of stirring potassium phosphate buffer (50 mM, pH 7.4 containing 1 mM EDTA, 2 mM beta-mercaptoethanol, 0.1 mM pyridoxal 5'-phosphate, and 0.1 M NaCl) |
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