EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
---|
2.3.2.13 | 0.00083 |
- |
Nalpha-benzyloxycarbonyl-L-Gln-Gly |
Km above 0.03 mM, mutant enzyme C371A, in 1 M MOPS, 5 mM EDTA, and 50 mM 2-oxoglutarate (pH 7.2), 100 mM CaCl2, at 37°C |
719877 |
2.3.2.13 | 0.0055 |
- |
Nalpha-benzyloxycarbonyl-L-Gln-Gly |
Km above 0.03 mM, mutant enzyme C370A, in 1 M MOPS, 5 mM EDTA, and 50 mM 2-oxoglutarate (pH 7.2), 100 mM CaCl2, at 37°C |
719877 |
2.3.2.13 | 0.015 |
- |
Nalpha-benzyloxycarbonyl-L-Gln-Gly |
mutant enzyme C230A, in 1 M MOPS, 5 mM EDTA, and 50 mM 2-oxoglutarate (pH 7.2), 100 mM CaCl2, at 37°C |
719877 |
2.3.2.13 | 0.017 |
- |
Nalpha-benzyloxycarbonyl-L-Gln-Gly |
wild type enzyme, in 1 M MOPS, 5 mM EDTA, and 50 mM 2-oxoglutarate (pH 7.2), 100 mM CaCl2, at 37°C |
719877 |
2.3.2.13 | 0.073 |
- |
Z-Gln-Gly |
recombinant wild-type enzyme, pH 7.2, 37°C |
758966 |
2.3.2.13 | 0.079 |
- |
N-CBZ-Glu-Gly |
pH 6.0, 37°C |
759632 |
2.3.2.13 | 0.143 |
- |
Z-Gln-Gly |
recombinant mutant S2P, pH 7.2, 37°C |
758966 |
2.3.2.13 | 0.67 |
- |
Nalpha-benzyloxycarbonyl-L-glutaminylglycine |
in 250 mM Tris acetate buffer (pH 8.0), at 37°C |
720000 |
2.3.2.13 | 1.34 |
- |
hydroxylamine |
pH 6.0, 37°C |
759632 |
2.3.2.13 | 2.26 |
- |
pentylamine |
pH 8.5, 4°C |
759209 |