1.2.1.59 | D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+ = 3-phospho-D-glyceroyl phosphate + NAD(P)H + H+ |
GAPDH catalyzes the oxidative phosphorylation of D-glyceraldehyde 3-phosphate (D-G3H) to give 1,3-diphosphoglyceric acid and uses NAD+ or NADP+ as a cofactor. In the first step of this two-step reaction the active-site cysteine residue of GAPDH attaches covalently to D-G3H and forms a thiohemiacetal intermediate, which transfers a hydride ion to NAD+, resulting in the formation of the thioacyl enzyme. In the second step the resulting thioester is phosphorylated through the nucleophilic attack of an inorganic phosphate ion on the carbonyl C atom of the thioacyl group, which leads to the formation of 1,3-diphosphoglycerate |
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