EC Number   |
|---|
    2.5.1.56 | - |
| 2.5.1.56 | cell harvesting by centrifugation, resuspension, lysing in high-pressure homogenizer, centrifugation, chromatographic procedures including ion-exchange and gel filtration steps, concentration |
| 2.5.1.56 | expression of the native neuB gene product enzyme in E. coli results in a product that is prone to proteolysis during purification so the protein is tagged with a hexa-histidine tag at its N-terminus and rapidly purified |
| 2.5.1.56 | Ni2+ affinity column chromatography |
| 2.5.1.56 | recombinant His-tagged enzyme from Escherichia coli strain Rosetta 2 by ultrafiltration, nickel affinity chromatography, and gel filtration |
| 2.5.1.56 | recombinant His6-tagged enzyme from Escherichia coli strain Rosetta 2 (DE3) pLysS by nickel affinity chromatography and gel filtration |
