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Search Purification (Commentary)

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EC Number Purification (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.101-
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.101affinity chromatography
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.101amylose resin column chromatography, gel filtration
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.101antibody purification from homogenized transfected Nicotiniana benthamiana leaves, resuspended in extraction buffer (PBS, pH 7.2, 1% Triton-X-100, protease inhibitor cocktail), centrifugation, supernatant incubated with rProtein A-Sepharose, elution by boiling Laemmli sample buffer, protein gel blot analysis with specific antibodies
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.101large scale, recombinant maltose-binding fusion protein
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.101liver
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.101mutant enzyme D144N
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.101one Ni2+-NTA chromatography step is sufficient for oligosaccharide synthesis
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.101partial
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.101recombinant catalytic fragment from Sf9 insect cells
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