EC Number |
---|
2.3.1.258 | HiTrap SP column chromatography, and Superdex 75 gel filtration |
2.3.1.258 | nickel affinity column chromatography |
2.3.1.258 | recombinant C-terminally His6-tagged enzyme RimI from Escherichia coli by nickel affinity chromatography and gel filtration |
2.3.1.258 | recombinant GST-tagged full-length Naa50p from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography and cleavage of the GST-tag by TEV protease, followed by ion exchange chromatography and gel filtration |
2.3.1.258 | recombinant GST-tagged hNaa50 and hNatA fromSf9 insect cells by affinity chromatography and gel filtration |
2.3.1.258 | recombinant GST-tagged SpNatA and SpNaa50 from Escherichia coli by glutathione affinity chromatography and gel filtration, SpNaa50 maintains the ability to co-migrate with SpNatA in sizing buffer with NaCl concentration as high as 1 M |
2.3.1.258 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration |
2.3.1.258 | recombinant hNaa15 mutants, hNaa50, and hNatA by affinity chromatography and gel filtration |