EC Number   |
|---|
   2.3.1.135 | affinity labeling with all-trans-retinyl-alpha-bromoacetate, SDS electrophoresis, prior to affinity labelling, incubation with cholesterol chloroacetate to block nucleophilic reagents, or streptavidin column to remove endogenously biotinylated proteins enhance protein purification |
| 2.3.1.135 | affinity labeling with N-Boc-L-biocytinyl-11-aminoundecan chloromethylketone, precipitation and gel electrophoresis, prior to affinity labelling, incubation with cholesterol chloroacetate to block nucleophilic reagents, or streptavidin column to remove endogenously biotinylated proteins enhance protein purification |
| 2.3.1.135 | enzyme transfected in HEK-293T cells partially purificated by solubilization and centrifugation |
| 2.3.1.135 | histidine-tagged truncated LRAT |
| 2.3.1.135 | labeling with N-Boc-L-biocytinyl-11-aminoundecan chloromethylketone, affinity chromatography, avidin, prior to affinity chromatography or affinity labelling, incubation with cholesterol chloroacetate to block nucleophilic reagents, or streptavidin column to remove endogenously biotinylated proteins enhance protein purification |
| 2.3.1.135 | native enzyme partially by membrane preparation |
| 2.3.1.135 | native enzyme partially by membrane preparation from retinal pigment epithelium |
| 2.3.1.135 | native enzyme partially by microsome preparation from eyes, recombinant enzyme partially from HEK-293T cells by membrane preparation |
| 2.3.1.135 | recombinant His-tagged LRAT by affinity chromatography |
| 2.3.1.135 | recombinant His-tagged truncated wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography in presence of 1% SDS |
