EC Number |
---|
1.4.1.1 | - |
1.4.1.1 | gene cloned and expressed in Escherichia coli |
1.4.1.1 | native enzyme |
1.4.1.1 | native enzyme by ammonium sulfate fractionation, dialysis, anion exchange chromatography, again dialysis, and gel filtration |
1.4.1.1 | Ni-NTA resin column chromatography |
1.4.1.1 | partial |
1.4.1.1 | recombinant enzyme |
1.4.1.1 | recombinant GST-tagged enzyme from Escherichia coli strain JM109 by glutathione affinity chrmatography |
1.4.1.1 | recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography and dialysis |
1.4.1.1 | recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography |