EC Number |
---|
1.14.20.1 | - |
1.14.20.1 | affinity chromatography |
1.14.20.1 | anion-exchange and gel filtration |
1.14.20.1 | efficient large-scale purification |
1.14.20.1 | expression in Escherichia coli, overexpression as an insoluble and inactive enzyme, elaboration of refolding scheme resulting in highliy active and moderately stable enzyme |
1.14.20.1 | native enzyme, partial, recombinant enzyme, to near homogenity |
1.14.20.1 | partial |
1.14.20.1 | purification of partially folded recombinant expandase/hydroxylase from insoluble granules of recombinant Escherichia coli |
1.14.20.1 | purification of refolding-competent recombinant enzyme |
1.14.20.1 | recombinant enzyme expressed in Escherichia coli |