EC Number |
---|
1.1.99.1 | from rat liver mitochondria, purification methods, overview |
1.1.99.1 | partial |
1.1.99.1 | partial, using Triton-treatment, chloroform-treatment and column chromatography on Sepharose 6B |
1.1.99.1 | recombinant enzyme from Escherichia coli |
1.1.99.1 | recombnant enzyme from Escherichia coli strain M15 by ammonium sulfate fractionation and anion exchange chromatography |
1.1.99.1 | using 0.5% Brij 58 treatment, column chromatography on DEAE-cellulose and gel filtration on Toyopearl HW-60S |
1.1.99.1 | using column chromatography of the chemically modified enzyme with 5,5'-dithiobis(2-nitrobenzoic acid) on DEAE-Sepharose CL-6B and choline-Sepharose 4B with C3-spacer, subsequent release of the thionitrobenzoate with dithiothreitol, and a second column chromatography on DEAE-Sepharose CL-6B in the presence of 0.1% Triton X-100 |
1.1.99.1 | using column chromatography on thio-hexyl and on an anion exchanger |