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EC Number
Purification (Commentary)
1.1.1.318
native EGS 118fold from petals by alternating steps of anion exchange and hydrophobic interaction chromatography, co-purification with isoeugenol synthase, EC 1.1.1.319. Recombinant His-tagged CbEGS1 and CbEGS2 from Escherichia coli by nickel affinity chromatography
1.1.1.318
recombinant GST-taged enzyme from Escherichia coli strain BL21 by glutathione affinity chromatography and dialysis
1.1.1.318
recombinant GST-tagged enzyme EGS1 from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography
1.1.1.318
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography
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