EC Number |
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1.1.1.198 | ammonium sulfate precipitation, phenyl Sepharose column chromatography and Q-Sepharose column chromatography |
1.1.1.198 | ammonium sulfate saturation, phenyl Sepharose column chromatography, and Q Sepharose column chromatography |
1.1.1.198 | His-Trap column chromatography |
1.1.1.198 | HisTrap column chromatography |
1.1.1.198 | near homogeneity, chromatography techniques, affinity chromatography |
1.1.1.198 | Ni-NTA affinity column chromatography |
1.1.1.198 | partial |
1.1.1.198 | partial, using Phenyl-Sepharose hydrophobic interaction, Q-Sepharose Fast Flow and Mono-Q anion-exchange columns |
1.1.1.198 | phenyl-Sepharose column chromatography, Q-Sepharose column chromatography and MonoQ column chromatography |
1.1.1.198 | production of enzyme in the form of inclusion body in Escherichia coli. The refolded BDH1 tends to precipitate. Insoluble recombinant BDH1 is converted into a soluble form by adding glycerol in LB medium |