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EC Number Application Commentary Reference
Display the reaction diagram Show all sequences 2.6.1.B17synthesis engineering of Escherichia coli for biosynthesis of L-phenylglycine from glucose. The enzymes HmaS (L-4-hydroxymandelate synthase), Hmo (L-4-hydroxymandelate oxidase) and HpgT (L-4-hydroxyphenylglycine transaminase) are heterologously expressed in Escherichia coli. HpgT conversing phenylglyoxylate to L-phenylglycine uses an unusual aminodonor L-phenylalanine, which releases another phenylpyruvate as the substrate of HmaS. A recycle-reaction maximizes the utilization of precursor phenylpyruvate. After deletionof tyrB and aspC, L-phenylglycine yield increases by 12.6fold. The L-phenylglycine yield is further improved by 14.9fold after enhancing hmaS expression 738735
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