EC Number |
General Stability |
Reference |
---|
2.7.1.11 | (NH4)2SO4 prevents enzyme from dissociation at pH 6.4 |
640423 |
2.7.1.11 | 10% glycerol, dithiothreitol and PMSF stabilize during purification |
640482 |
2.7.1.11 | 50 mM phosphate stabilizes at pH 6.6 |
640432 |
2.7.1.11 | AMP, ADP, fructose 6-phosphate or antipain stabilizes during purification |
640452 |
2.7.1.11 | at least 15 mM phosphate required to stabilize PFK during purification, presence of 10% glycerol helps to stabilize |
640448 |
2.7.1.11 | ATP stabilizes |
640445 |
2.7.1.11 | cold labile, fructose 1,6-bisphosphate stabilizes |
640434 |
2.7.1.11 | concentration by ultrafiltration on Amicon PM-10 membrane results in appreciable loss of activity, concentration in collodium bags allows higher protein concentration to be obtained without loss of activity |
640452 |
2.7.1.11 | cytosolic enzyme extremely unstable upon purification, substrates, metabolites, ethylene glycol or SH-compounds do not stabilize |
640444 |
2.7.1.11 | cytosolic enzymes are less stable than plastidic |
640440 |