EC Number   |
General Stability |
Reference |
|---|
    2.1.2.1 | 2-mercaptoethanol stabilizes |
441403, 441408 |
| 2.1.2.1 | above 6 M urea the dichrpoic activity is reduced significantly with a transition midpoint at 2.56 M. This process is completely reversible |
659269 |
| 2.1.2.1 | bsSHMT has six unconserved lysine residues in C-terminal domain which render it more resistant to alkaline denaturation. Chemical modification of lysine side chains results in stabilization of monomers |
718968 |
| 2.1.2.1 | dialysis inactivates |
441408 |
| 2.1.2.1 | DTT stabilizes |
441403, 441413 |
| 2.1.2.1 | EDTA stabilizes |
441403, 441408 |
| 2.1.2.1 | folate stabilizes |
441391 |
| 2.1.2.1 | glycine betaine protects the ApSHMT enzyme activity in vitro |
719529 |
| 2.1.2.1 | guanidinium chloride-induced two-step unfolding of SHM1 with the first step being dissociation of dimer into apomonomer at low denaturant concentrations followed by unfolding of the stabilized monomer at higher denaturant concentrations, SHM1 |
659265 |
| 2.1.2.1 | guanidinium chloride-induced two-step unfolding of SHM1 with the first step being dissociation of dimer into apomonomer at low denaturant concentrations followed by unfolding of the stabilized monomer at higher denaturant concentrations, SHM2 |
659265 |
