3.1.4.3 phospholipase C blastodisc in vitro vasculogenesis model. Activities of both Ca2+-dependent and independent isoform PC-PLC and the level of reactive oxygen species are elevated during the endothelial differentiation of the blastodisc. Ca2+-independent PC-PLC undergoes an increase in 24 h, then decreases gradually with the cell differentiation, while Ca2+-dependent PC-PLC is almost not changed during the process 681024