1.1.1.1 alcohol dehydrogenase culture condition adhA transcription is induced by ethanol or n-propanol, adhA transcription is subject to glucose catabolite repression. Accordingly, both induction of AdhA activity and ethanol utilization are detected only after depletion of glucose 684612 1.1.1.337 L-2-hydroxycarboxylate dehydrogenase (NAD+) culture condition maximum specific and volume activities are found at the end of the exponential growth phase, during further fermentation enzyme activity declines rapidly and is no longer detectable after 15 h 726750 1.1.1.368 6-hydroxycyclohex-1-ene-1-carbonyl-CoA dehydrogenase culture condition when cells are grown aerobically with benzoate the activity is 20-25fold lower than in cells grown anaerobically with benzoate 724904 1.1.1.49 glucose-6-phosphate dehydrogenase (NADP+) culture condition KlZWF1 is constitutively expressed. Its deletion leads to increased sensitivity to hydrogen peroxide on glucose. The Klzwf1DELTA strain has a reduced biomass yield on fermentative carbon sources as well as on lactate and glycerol 686466 1.1.1.49 glucose-6-phosphate dehydrogenase (NADP+) culture condition the highest glucose 6-phosphate dehydrogenase activity occurrs when the glucose solution is fed into the fermenter through the decreasing linear mode 684518 1.1.1.51 3(or 17)beta-hydroxysteroid dehydrogenase culture condition exposure of the fish to water-soluble fraction of crude oil produces an apparent concentration-specific increase of 3beta-hydroxysteroid dehydrogenase 686428 1.1.3.13 alcohol oxidase culture condition when incubated in the cholesterol medium, the alcohol oxidase activity reaches the maximum at 2 days of cultivation, and then gradually decreases. Methanol, ethanol and isopropanol are also effective for production of alcohol oxidase, but glycerol, glucose and malic acid are not -, 688123 1.1.3.6 cholesterol oxidase culture condition the strain reaches its maximal utilization of cholesterol as the only C source for production of extracellular cholesterol oxidase 684242 1.1.3.9 galactose oxidase culture condition maximum galactose oxidase production (approximately 4.0 U/ml) is obtained when fermentation is carried out at 25°C, with orbital shaking (100 rpm) and an initial medium of pH 7.0, for 96 h, using a 2% (v/v) inoculum made from a homogenized four-day-old liquid culture, in the presence of copper, manganese, and magnesium 687434 1.10.3.2 laccase culture condition fungal cultures grown in malt extract medium -, 684626 1.10.3.2 laccase culture condition malt extract is the carbon source which yields the highest laccase production, with a maximum of laccase production reached after 9 days of cultivation. The nitrogen source soya peptone yields the highest laccase production 687338 1.11.1.13 manganese peroxidase culture condition grown on banana waste -, 765837 1.11.1.13 manganese peroxidase culture condition grown on bark mulch and wood chips 765837 1.11.1.13 manganese peroxidase culture condition grown on dyes containing agar plates -, 765837 1.11.1.13 manganese peroxidase culture condition grown on GPYM agar plates 765837 1.11.1.13 manganese peroxidase culture condition grown on Japanese beech and cedar wood, Eucalyptus grandis wood, and Bamboo culms 765837 1.11.1.13 manganese peroxidase culture condition grown on MEG agar slant -, 765837 1.11.1.13 manganese peroxidase culture condition grown on mineral salt media 765837 1.11.1.13 manganese peroxidase culture condition grown on potato dextrose agar -, 765837 1.11.1.13 manganese peroxidase culture condition grown on potato dextrose broth (PDB) 765837 1.11.1.13 manganese peroxidase culture condition grown on solid-state fermentation medium -, 765837 1.11.1.13 manganese peroxidase culture condition grown on wheat straw, rice straw, agriculture byproducts, or agro-industrial wastes 765837 1.11.1.13 manganese peroxidase culture condition strain Al-Dhabi 140 is grown in mineral salt medium containing 25, 50, 75, 100, 125, 150, 175 and 200 mg/l of tetracycline with 7% inoculum under shaking condition at 150 rpm.Tetracycline concentration in the culture medium is assayed after eight days. The degradation potential by strain Al-Dhabi 140 is 71 mg/l and 75.3 mg/l when the tetracycline concentration is 125 and 150 mg/l, respectively -, 764508 1.13.11.1 catechol 1,2-dioxygenase culture condition monocyclic hydrocarbons, phenol, catechol, benzoic acid and vanillic acid -, 702976 1.13.11.2 catechol 2,3-dioxygenase culture condition monocyclic hydrocarbons, phenol, catechol, benzoic acid, protocatechuic acid and vanillic acid -, 702976 1.13.11.3 protocatechuate 3,4-dioxygenase culture condition monocyclic hydrocarbons, 4-hydroxybenzoic acid, protocatechuic acid and vanillic acid -, 702976 1.3.1.33 protochlorophyllide reductase culture condition low expression, light-response during greening, expression in mature cells 656908 2.4.1.49 cellodextrin phosphorylase culture condition cells grown in continuous culture under cellobiose or cellulose limitation over a 10fold range of dilution rates. The gene displays modest difference in expression with growth rate or substrate type 671420 3.2.1.132 chitosanase culture condition medium containing shrimp heads as the sole carbon and nitrogen source -, 751450 3.2.1.132 chitosanase culture condition the highest level of enzyme activity (186 U/ml) is achieved in culture medium using high cell-density cultivation in a 7-l fermenter. Chitosanase is successfully secreted to the culture media through the widely used SecB-dependent type II pathway in Escherichia coli 749607 3.4.19.13 glutathione gamma-glutamate hydrolase culture condition fermentation broth of carbon stressed cultures -, 754814 3.5.1.1 asparaginase culture condition carbon sources such as sucrose, maltose, galactose, lactose, mannitol and mannose inhibit enzyme production. Exogenous cAMP in presence of carbon sources stimulates L-asparaginase enzyme production 686239 3.5.1.1 asparaginase culture condition high L-asparaginase activity is found in cells cultured on L-fructose, D-galactose, sucrose or maltose, and in cells cultured on L-asparagine as the sole nitrogen source 686239 3.5.1.1 asparaginase culture condition L-asparaginase-I is constitutive 686239 3.5.1.1 asparaginase culture condition L-asparaginase-II is secreted in response to N starvation 686239 3.5.1.1 asparaginase culture condition sabourand dextrose broth yields maximum growth and maximum L-asparaginase production 686239 3.5.1.11 penicillin amidase culture condition 51 g/l of casein hydrolyzed with Alcalase and 2.7 g/l of phenylacetic acid (PhAc), the following carbon substrates are tested, individually and combined: glucose, glycerol, and lactose (present in cheese whey). Glycerol and glucose are effective nutrients for the microorganism growth but delay the penicillin G acylase production. Cheese whey always increases enzyme production and cell mass. However lactose (present in cheese whey) is not a significant carbon source for Bacillus megaterium. Phenylacetic acid, amino acids, and small peptides present in the hydrolyzed casein are the actual carbon sources for enzyme production. Replacement of hydrolyzed casein by free amino acids, 10.0 g/l, leads to a significant increase in enzyme production (approximately 150%), with a preferential consumption of alanine, aspartic acid, glycine, serine, arginine, threonine, lysine, and glutamic acid. A decrease of the enzyme production is observed when 20.0 g/l of amino acids are used. Using the single omission technique, it is shown that none of the 18 tested amino acids is essential for enzyme production. The use of a medium containing eight of the preferentially consumed amino acids leads to similar enzyme production level obtained when using 18 amino acids. Phenylacetic acid, up to 2.7 g/l, does not inhibit enzyme production, even if added at the beginning of the cultivation 685709 3.5.1.2 glutaminase culture condition optimizing concentration of sucrose, yeast extract, glutamine, and sodium chloride for L-glutaminase production by response surface methodology 685637 3.5.1.23 ceramidase culture condition when the strain is cultured with sphingomyelin or phosphatidylcholine, production of the enzyme drastically increases, causing the increase of hemolytic activity in the cellfree culture supernatant. Ceramide and sphingosine are effective in promoting the production of ceramidase -, 680936 3.5.1.41 chitin deacetylase culture condition chitin as sole carbon resource of culture medium. The enzyme activity is 10-11 units/ml culture supernatant after the strain is shaken at 200 rpm and 29°C for 96 h 685962 4.2.1.84 nitrile hydratase culture condition optimum growth temperature of the bacterium is 25°C to 30°C, but it is able to grow at 4°C 729043 4.3.1.B2 imidazole glycerol phosphate synthase culture condition the production of the soluble HIS7 is highest at 25°C with 1 mM isopropyl beta-D-thiogalactopyranoside and this condition is used for the large-scale protein preparations 728731 5.6.2.2 DNA topoisomerase (ATP-hydrolysing) culture condition actively dividing Sulfolobus solfataricus cells contain only small amounts of both reverse gyrases, approximately 50 TopR1 and 125 TopR2 molecules per cell at 80°C. Sulfolobus solfataricus cells are resistant at 45°C for several weeks, but there is neither cell division nor replication initiation; these processes are fully restored upon a return to 80°C. TopR1 is not found after three weeks at 45°C whereas the amount of TopR2 remains constant -, 729504 6.2.1.40 4-hydroxybutyrate-CoA ligase (AMP-forming) culture condition cells grown under strict H2-CO2 autotrophy -, 725542