1.1.1.10 L-xylulose reductase cell culture - -, 740206 1.1.1.100 3-oxoacyl-[acyl-carrier-protein] reductase cell culture strain KCTC1639 693287 1.1.1.12 L-arabinitol 4-dehydrogenase cell culture strain 5XY2 catabolizes L-arabinose as well as D-glucose and D-xylose in fermentation -, 738728 1.1.1.120 galactose 1-dehydrogenase (NADP+) cell culture - -, 285790, 285791, 285792 1.1.1.121 aldose 1-dehydrogenase (NAD+) cell culture cells grown on various sugars, D-fucose and D-glucose are effective inducers of D-aldohexose dehydrogenase, D-galactose induces only slightly, L-arabinose is no inducer -, 285794 1.1.1.122 D-threo-aldose 1-dehydrogenase cell culture - -, 285798, 285800, 285802 1.1.1.122 D-threo-aldose 1-dehydrogenase cell culture inducible 285801 1.1.1.124 fructose 5-dehydrogenase (NADP+) cell culture - 285814, 285815, 285816, 285817 1.1.1.125 2-deoxy-D-gluconate 3-dehydrogenase cell culture inducible enzyme synthesis by growth in 2-deoxy-D-gluconate containing medium 285818 1.1.1.126 2-dehydro-3-deoxy-D-gluconate 6-dehydrogenase cell culture inducible enzyme synthesis by growth in alginate-containing medium 285819 1.1.1.127 2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase cell culture - 285820, 285821 1.1.1.129 L-threonate 3-dehydrogenase cell culture inducible by growing in L-threonate-containing medium 285825 1.1.1.130 3-dehydro-L-gulonate 2-dehydrogenase cell culture - 285827 1.1.1.135 GDP-6-deoxy-D-talose 4-dehydrogenase cell culture - 285847 1.1.1.138 mannitol 2-dehydrogenase (NADP+) cell culture - -, 285855, 94717 1.1.1.14 L-iditol 2-dehydrogenase cell culture electrophoretic karyotyping and array-based comparative genomic hybridization (array-CGH), comparison of four different species derived from the Saccharomyces sensu stricto complex of 22 distillery strains, overview. The genomic diversity is mainly revealed within subtelomeric regions and the losses and/or gains of fragments of chromosomes I, III, VI and IX are the most frequently observed. Statistically significant differences in the gene copy number are documented in six functional gene categories: 1. telomere maintenance via recombination, DNA helicase activity or DNA binding, 2. maltose metabolism process, glucose transmembrane transporter activity, 3. asparagine catabolism, cellular response to nitrogen starvation, localized in cell wall-bounded periplasmic space, 4. siderophore transport, 5. response to copper ion, cadmium ion binding and 6. L-iditol 2-dehydrogenase activity. Distillery yeasts are diploid. Gene ontology overrepresentation profiles are species-specific 741083 1.1.1.15 D-iditol 2-dehydrogenase cell culture - 389403 1.1.1.152 3alpha-hydroxy-5beta-androstane-17-one 3alpha-dehydrogenase cell culture - 286009 1.1.1.154 ureidoglycolate dehydrogenase cell culture - 286034 1.1.1.16 galactitol 2-dehydrogenase cell culture - 287244, 389406 1.1.1.166 hydroxycyclohexanecarboxylate dehydrogenase cell culture - 286100 1.1.1.17 mannitol-1-phosphate 5-dehydrogenase cell culture - -, 285907, 287248, 389412, 389413, 389414, 389416, 389417, 389418, 389419, 389421, 389423, 389426 1.1.1.17 mannitol-1-phosphate 5-dehydrogenase cell culture inducible by D-mannitol 285902, 389415 1.1.1.17 mannitol-1-phosphate 5-dehydrogenase cell culture reductase activity affected by the presence of nitrate during growth 389424 1.1.1.18 inositol 2-dehydrogenase cell culture - -, 389427, 389428, 389429, 389430, 389431, 389432, 389433, 389434, 389435, 389436, 5706 1.1.1.187 GDP-4-dehydro-D-rhamnose reductase cell culture - 389366 1.1.1.191 indole-3-acetaldehyde reductase (NADPH) cell culture maximum enzyme activity from cultures after 48 h 286157 1.1.1.193 5-amino-6-(5-phosphoribosylamino)uracil reductase cell culture - -, 246736 1.1.1.195 cinnamyl-alcohol dehydrogenase cell culture - 670974 1.1.1.195 cinnamyl-alcohol dehydrogenase cell culture xylem-derived 670603 1.1.1.201 7beta-hydroxysteroid dehydrogenase (NADP+) cell culture - -, 286224, 286226, 286228, 286229 1.1.1.201 7beta-hydroxysteroid dehydrogenase (NADP+) cell culture bile salt induction of enzymes synthesis 286076, 286227 1.1.1.203 uronate dehydrogenase cell culture - 286230, 286231 1.1.1.205 IMP dehydrogenase cell culture - 347916 1.1.1.22 UDP-glucose 6-dehydrogenase cell culture - 670630, 740715 1.1.1.236 tropinone reductase II cell culture root culture 656413 1.1.1.237 hydroxyphenylpyruvate reductase cell culture - 657023 1.1.1.247 codeinone reductase (NADPH) cell culture - 9551, 9553 1.1.1.255 mannitol dehydrogenase cell culture - 286399, 286400, 286401, 286402 1.1.1.255 mannitol dehydrogenase cell culture enzyme activity is repressed in cells grown in various amendments of hexoses, sugar repression appears to be mediated by hexokinases 286406 1.1.1.255 mannitol dehydrogenase cell culture pathogen-induced endogenous enzyme 286407 1.1.1.256 fluoren-9-ol dehydrogenase cell culture use of fluorene as the sole source of carbon and energy -, 207942, 207944, 94344 1.1.1.257 4-(hydroxymethyl)benzenesulfonate dehydrogenase cell culture - -, 207945, 207946, 207947, 207948, 207949 1.1.1.259 3-hydroxypimeloyl-CoA dehydrogenase cell culture - -, 207950, 207951, 207952 1.1.1.260 sulcatone reductase cell culture - -, 207953, 207954, 207955 1.1.1.261 sn-glycerol-1-phosphate dehydrogenase cell culture - -, 207956, 207957, 207958, 207959, 207960, 207961 1.1.1.264 L-idonate 5-dehydrogenase cell culture - 285822, 285823, 285824 1.1.1.265 3-methylbutanal reductase cell culture - 246373, 246375, 286203 1.1.1.268 2-(R)-hydroxypropyl-CoM dehydrogenase cell culture - -, 288637 1.1.1.268 2-(R)-hydroxypropyl-CoM dehydrogenase cell culture metabolism of propylene depends on the presence of a linear megaplasmid, that encodes enzymes of alkene oxidation, epoxide carboxylation and CoM biosynthesis -, 389508 1.1.1.269 2-(S)-hydroxypropyl-CoM dehydrogenase cell culture metabolism of propylene depends on the presence of a linear megaplasmid, that encodes enzymes of alkene oxidation, epoxide carboxylation and CoM biosynthesis -, 389508 1.1.1.27 L-lactate dehydrogenase cell culture FBP-activated L-nLDH activity is constantly very significant throughout the growth period, even when lactate is consumed in the stationary phase of respiration. During the lactate utilization period, L-nLDH activity is regulated by some factors and the pyruvate reduction activity is completely inhibited or masked -, 740458 1.1.1.27 L-lactate dehydrogenase cell culture steady-state growth at pH 7 and pH 5 -, 740628 1.1.1.28 D-lactate dehydrogenase cell culture 42°C, addition of 5 g/l peptone and 1 g/l (NH4)2HPO4 enhance D-lactic acid production by 32%, as compared to that obtained from non supplemented media, with a productivity of 3.0 g/l/h. Lactate dehydrogenase (LDH) expression profile in the different media, and effects of various nitrogen sources on D-lactic acid production, overview -, 740170 1.1.1.306 S-(hydroxymethyl)mycothiol dehydrogenase cell culture - -, 288322, 288323, 654527 1.1.1.306 S-(hydroxymethyl)mycothiol dehydrogenase cell culture induction of enzyme and factor synthesis only if cells grown in methanol-containing medium 288324 1.1.1.307 D-xylose reductase [NAD(P)H] cell culture - -, 740206 1.1.1.31 3-hydroxyisobutyrate dehydrogenase cell culture astroglia-rich primary culture from neonatal rats 688515 1.1.1.312 2-hydroxy-4-carboxymuconate semialdehyde hemiacetal dehydrogenase cell culture - -, 671526 1.1.1.34 hydroxymethylglutaryl-CoA reductase (NADPH) cell culture - -, 740956, 740970 1.1.1.4 (R,R)-butanediol dehydrogenase cell culture - -, 722559 1.1.1.40 malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) cell culture only two isozymes present in the fermenter-grown or bottle-grown cultures 689116 1.1.1.406 galactitol 2-dehydrogenase (L-tagatose-forming) cell culture - 389404 1.1.1.42 isocitrate dehydrogenase (NADP+) cell culture - 656588 1.1.1.44 phosphogluconate dehydrogenase (NADP+-dependent, decarboxylating) cell culture - 696574 1.1.1.6 glycerol dehydrogenase cell culture - -, 668061, 674122, 674272, 675794 1.1.1.67 mannitol 2-dehydrogenase cell culture glucose as carbon source represses mannitol dehydrogenase -, 287240 1.1.1.67 mannitol 2-dehydrogenase cell culture glucose induces enzyme production 287252, 287256 1.1.1.8 glycerol-3-phosphate dehydrogenase (NAD+) cell culture - -, 671463, 723769 1.1.1.81 hydroxypyruvate reductase cell culture axenic cultures 695413 1.1.1.9 D-xylulose reductase cell culture strain 5XY2 catabolize L-arabinose as well as D-glucose and D-xylose in fermentation -, 738728 1.1.2.3 L-lactate dehydrogenase (cytochrome) cell culture optimization of culture conditions for recombinant cells of strain C-105 expressing gene CYB2 and showing increased FCb2 activity 700066 1.1.3.15 (S)-2-hydroxy-acid oxidase cell culture - 389675, 389699 1.1.3.28 nucleoside oxidase cell culture - -, 287611, 287612, 287613, 287614 1.1.3.39 nucleoside oxidase (H2O2-forming) cell culture - -, 287628 1.1.3.39 nucleoside oxidase (H2O2-forming) cell culture enhanced production of H2O2-forming nucleoside oxidase by addition of Fe2+, Fe3+ and Cu2+ to the culture medium 287627 1.1.3.6 cholesterol oxidase cell culture - 287646, 287648, 287658 1.1.3.6 cholesterol oxidase cell culture production of cholesterol oxidase under batch conditions through Ca-alginate immobilized cells of Streptomyces sp., optimization of immobilization method and production conditions, overview. The enzyme production with immobilized cells is higher in comparison to free cells under optimized conditions. Production medium inoculated with 2% v/v or 8.8 mg dry cell weight seed culture and incubated at 200 rpm and 30°C for 96 h gves maximum COD enzyme production after 96 h whereas the immobilized culture gives maximum production at 37°C and 300 rpm after 72 h of incubation. The immobilized cells can be used for three consecutive fermentation cycles for COD production in higher quantities as compared with free cells 742798 1.1.3.9 galactose oxidase cell culture - 389864, 389866, 389873 1.1.5.5 alcohol dehydrogenase (quinone) cell culture the cells are able to grow on up to 10% acetic acid, expression analysis, overview -, 686622 1.1.5.5 alcohol dehydrogenase (quinone) cell culture the cells show high enzyme activity 684589 1.1.5.5 alcohol dehydrogenase (quinone) cell culture the MSU10 strain shows higher acetic acid productivity in a medium containing 6% ethanol at 37°C than strain SKU1108, while the SKU1108 strain can accumulate more acetic acid in a medium supplemented with 4-5% ethanol at the same temperature. The fermentation ability at 37°C of these thermotolerant strains is superior to that of mesophilic strains IFO3191 and IFO3284 having weak growth and very delayed acetic acid production at 37°C even at 4% ethanol -, 724042 1.1.5.8 quinate/shikimate dehydrogenase (quinone) cell culture - 672681 1.1.99.2 L-2-hydroxyglutarate dehydrogenase cell culture high levels of the enzyme is produced at the late stage of cultivation in the presence of citrate and with limited aeration 389902 1.10.3.3 L-ascorbate oxidase cell culture - 439906, 439907, 439909, 439913, 439937 1.11.1.13 manganese peroxidase cell culture production of ligninolytic enzyme MnP in liquid fermentation medium of Phanerochaete chrysosporium strain BKMF-1767 -, 765186 1.11.1.13 manganese peroxidase cell culture shallow stationary culture growing on N-limited medium 658782 1.11.1.6 catalase cell culture - -, 439772, 439773, 439777, 439778, 439779, 439784, 439786, 439787, 439789, 439790, 439792, 439794, 439799, 439800, 439801, 439802, 439807, 439808 1.11.1.6 catalase cell culture inducible at low salt concentrations 439785 1.11.1.6 catalase cell culture inducible by H2O2, heat-shock, ethanol and stationary-phase conditions 439782 1.11.1.6 catalase cell culture KatB inducible by H2O2 439780 1.11.1.6 catalase cell culture methanol grown -, 439791 1.11.1.6 catalase cell culture only present if growth medium is supplemented with hemin 439774 1.11.1.7 peroxidase cell culture phosphate-starved 663928 1.13.11.1 catechol 1,2-dioxygenase cell culture strain P8, ATCC 49451 690594 1.13.11.19 cysteamine dioxygenase cell culture grown in taurin-free or taurine-containing medium -, 743204 1.13.11.2 catechol 2,3-dioxygenase cell culture strain P8, ATCC 49451 690594 1.13.11.27 4-hydroxyphenylpyruvate dioxygenase cell culture - 395387 1.13.11.55 sulfur oxygenase/reductase cell culture - -, 742198 1.13.11.55 sulfur oxygenase/reductase cell culture best growth temperature is 65°C -, 743261 1.13.11.55 sulfur oxygenase/reductase cell culture strain TPY is cultured in the presence of ferrous sulfate (FeSO4) or elemental sulfur (S0) -, 742580 1.13.11.9 2,5-dihydroxypyridine 5,6-dioxygenase cell culture pyridine-2,6-dicarboxylic acid- and pyridine-2,3-dicarboxylic acid-induced strain 23K8 cells. The cells do not grow on pyridine-2,5-dicarboxylic acid -, 742294 1.14.11.28 proline 3-hydroxylase cell culture - -, 663681, 664403, 664814, 665361, 666959 1.14.12.18 biphenyl 2,3-dioxygenase cell culture - -, 693284 1.14.13.107 limonene 1,2-monooxygenase cell culture temperatures greater than 30°C inhibit growth completely. Growth rates on different substrates, overview -, 684540 1.14.13.128 7-methylxanthine demethylase cell culture soil bacterium Pseudomonas putida strain CBB5 can use caffeine (1,3,7-trimethylxanthine) as a sole carbon and nitrogen source -, 765239 1.14.13.128 7-methylxanthine demethylase cell culture strain NCIM 5235 can utilize caffeine as sole source of carbon and nitrogen for growth 763990 1.14.13.19 taxifolin 8-monooxygenase cell culture - 395990 1.14.13.25 methane monooxygenase (soluble) cell culture cultured in copper-limited NMS media -, 764425 1.14.13.8 flavin-containing monooxygenase cell culture primary hepatic amd gill epithelial cells 703402 1.14.13.82 vanillate monooxygenase cell culture - -, 207983, 639289, 639290 1.14.13.82 vanillate monooxygenase cell culture the cells grow in vanillate, 4-hydroxybenzoate, syringate, ferulate, p-coumarate, and 3-O-methylgallate, but not on vanillin and syringaldehyde. Maximum specific growth rates, uptake rates and yields on lignin model compounds by Microbacterium sp. RG1, overview. When strain is cultivated with 5 mM each of the aldehydes vanillin or syringaldehyde no growth is observed for more than 200 h 764310 1.14.13.B34 N-demethylase reductase cell culture soil bacterium Pseudomonas putida strain CBB5 can use caffeine (1,3,7-trimethylxanthine) as a sole carbon and nitrogen source -, 765239 1.14.13.B34 N-demethylase reductase cell culture strain NCIM 5235 can utilize caffeine as sole source of carbon and nitrogen for growth 763990 1.14.14.101 dihydrochelirubine 12-monooxygenase cell culture yeast-elicited cells 7393 1.14.14.102 N-methylcoclaurine 3'-monooxygenase cell culture - 660256 1.14.14.105 taxane 10beta-hydroxylase cell culture - 438774 1.14.14.106 taxane 13alpha-hydroxylase cell culture - 440248, 440249 1.14.14.142 8-dimethylallylnaringenin 2'-hydroxylase cell culture - 676359 1.14.14.19 steroid 17alpha-monooxygenase cell culture - 705396 1.14.14.20 phenol 2-monooxygenase (FADH2) cell culture strain UPV-1 is able to grow on phenol as the sole carbon and energy source -, 701875 1.14.14.21 dibenzothiophene monooxygenase cell culture grown on dibenzothiophene as sole sulfur source -, 392304 1.14.14.21 dibenzothiophene monooxygenase cell culture growth on dibenzothiophene as sole sulfur source, no or poor growth of strain KA2-5-1 with benzothiophene and various alkyl benzothiophenes, overview -, 736118 1.14.14.21 dibenzothiophene monooxygenase cell culture the organism can use dibenzothiophene as a sole source of sulfur for growth at 30-52°C, it can also utilize benzothiophene and thiophene as sulfur sources for growth. Range of organosulfur substrates utilized as a sole source of sulfur for growth by Mycobacterium phlei GTIS10, best is dibenzothiophene, overview -, 735525 1.14.14.22 dibenzothiophene sulfone monooxygenase cell culture grown on dibenzothiophene as sole sulfur source -, 392304 1.14.14.22 dibenzothiophene sulfone monooxygenase cell culture the organism can use dibenzothiophene as a sole source of sulfur for growth at 30-52°C, it can also utilize benzothiophene and thiophene as sulfur sources for growth. Range of organosulfur substrates utilized as a sole source of sulfur for growth by Mycobacterium phlei GTIS10, best is dibenzothiophene, overview -, 735525 1.14.14.82 flavonoid 3'-monooxygenase cell culture - 439004, 439010 1.14.14.83 geraniol 8-hydroxylase cell culture cell line C20D 716475 1.14.14.83 geraniol 8-hydroxylase cell culture G10H activity is correlated to the ability of the cells to accumulate terpenoid indole alkaloids. Alkaloid induction medium induces the enzyme, overview 718237 1.14.14.85 7-deoxyloganate 7-hydroxylase cell culture - 437786, 440296 1.14.14.89 4'-methoxyisoflavone 2'-hydroxylase cell culture suspension 285318 1.14.14.91 trans-cinnamate 4-monooxygenase cell culture - 660303 1.14.14.93 3,9-dihydroxypterocarpan 6a-monooxygenase cell culture - 390057, 390058, 390062 1.14.14.96 5-O-(4-coumaroyl)-D-quinate 3'-monooxygenase cell culture - 395989 1.14.15.1 camphor 5-monooxygenase cell culture Pseudomonas putida strains can use (1R)-(+) camphor as sole carbon and energy source 725873 1.14.16.2 tyrosine 3-monooxygenase cell culture - 671818, 672125, 672211, 672401, 674362, 676182 1.14.16.4 tryptophan 5-monooxygenase cell culture - 671723, 675511, 676182, 676184 1.14.17.1 dopamine beta-monooxygenase cell culture primary culture of trunk region of quail eggs, overview 689180 1.14.17.3 peptidylglycine monooxygenase cell culture - 672059, 672113, 673624, 674739, 676976, 745327 1.14.17.3 peptidylglycine monooxygenase cell culture primary anterior pituitary cells 745327 1.14.18.1 tyrosinase cell culture - 636350, 636368, 636375 1.14.19.35 sn-2 acyl-lipid omega-3 desaturase (ferredoxin) cell culture - 734558 1.14.19.39 acyl-lipid DELTA12-acetylenase cell culture cell growth between 4°C and 32°C 684454 1.14.19.51 (S)-corytuberine synthase cell culture - 687805 1.14.19.6 acyl-CoA (9+3)-desaturase cell culture - 659593 1.14.19.68 (S)-canadine synthase cell culture - 15195, 393236 1.14.19.68 (S)-canadine synthase cell culture protoberberine producing cell line 393236 1.14.20.5 flavone synthase I cell culture - 439113 1.14.20.5 flavone synthase I cell culture induced by continous irradiation with ultraviolet/blue light for 20 h 654335 1.15.1.1 superoxide dismutase cell culture - -, 438170 1.15.1.1 superoxide dismutase cell culture propagation of the fungus in the Mus musculus macrophage cell line derived from a reticulum sarcoma, J774.1, ATCC TIB-67, the enzyme expression is upregulated during macrophage infection 688805 1.15.1.1 superoxide dismutase cell culture the organism has a very high enzyme content 678510 1.16.9.1 iron:rusticyanin reductase cell culture cells grown anaerobically on S0 as an electron donor and Fe3+ as an electron acceptor, gene transcript profile of gene cyc2 monitored throughout the cells' growth phases. Reduction in the abundance of Cyc2 in anaerobic cells -, 746418 1.17.1.4 xanthine dehydrogenase cell culture - -, 644577, 644581, 644583, 644590, 644594, 644597, 644603, 644611, 644616, 644617 1.17.1.4 xanthine dehydrogenase cell culture 0.0025 mg/ml sodium tungstate in the growth medium reduces level of ferricyanide-linked enzyme to 3% 644593 1.17.1.4 xanthine dehydrogenase cell culture alveolar type II cells 644591 1.17.1.4 xanthine dehydrogenase cell culture inducible 644608 1.17.1.4 xanthine dehydrogenase cell culture inducible by hypoxanthine -, 644561, 644562 1.17.1.4 xanthine dehydrogenase cell culture inducible by urate, selenite and molybdate 644614 1.17.1.4 xanthine dehydrogenase cell culture inducible by urate, selenite and tungstate 644614 1.17.1.4 xanthine dehydrogenase cell culture inducible by xanthine 644570, 644573 1.17.1.4 xanthine dehydrogenase cell culture inducible by xanthine, hypoxanthine or adenine 644569 1.17.1.4 xanthine dehydrogenase cell culture inducible by xanthine, selenite and molybdate 644566 1.17.1.4 xanthine dehydrogenase cell culture selenium is needed for xanthine dehydrogenase formation 644592 1.17.1.5 nicotinate dehydrogenase cell culture strain T2 is capable of utilizing nicotinic acid as sole carbon and nitrogen source -, 745661 1.17.2.3 formate dehydrogenase (cytochrome-c-553) cell culture - -, 671992 1.17.2.3 formate dehydrogenase (cytochrome-c-553) cell culture in contrast to FDH2 enzyme the FDH3 complex lacks the gamma subunit and is unable to reduce cytochrome c-553 -, 671992 1.17.3.2 xanthine oxidase cell culture optimization of culture and growth conditions for Bacillus pumilus strain RL-2d, identified from screening as a strain with hyperactive xanthine oxidase. Medium M6 (pH 7.5, 55°C) containing (g/l) 10.0 g glucose, 3.0 g yeast extract, 1.0 g beef extract, 5.0 g peptone, 5.0 g sodium chloride and 0.152 g xanthine proves to be the best for the xanthine oxidase activity, overview -, 745064 1.17.3.2 xanthine oxidase cell culture optimization of culture condition for enzyme production, e.g. of the pH-shift strategy based on fermentation kinetic analysis. Kinetic models for cell growth, product accumulation, and substrate consumption are also analyzed and used to predict the behavior of fermentation system and to optimize XOD fermentation, optimum initial pH for XOD accumulation is pH 8.6, 30°C, overview -, 744517 1.18.1.2 ferredoxin-NADP+ reductase cell culture high- or low-CO2-grown (H or L)-cells 716442 1.2.1.10 acetaldehyde dehydrogenase (acetylating) cell culture - -, 672735 1.2.1.11 aspartate-semialdehyde dehydrogenase cell culture - 673074 1.2.1.11 aspartate-semialdehyde dehydrogenase cell culture highly expressed -, 670165 1.2.1.11 aspartate-semialdehyde dehydrogenase cell culture weakly expressed -, 670165 1.2.1.16 succinate-semialdehyde dehydrogenase [NAD(P)+] cell culture - 698627 1.2.1.19 aminobutyraldehyde dehydrogenase cell culture - -, 661760 1.2.1.22 lactaldehyde dehydrogenase cell culture - 670833, 674270, 675430 1.2.1.24 succinate-semialdehyde dehydrogenase (NAD+) cell culture - 674654 1.2.1.27 methylmalonate-semialdehyde dehydrogenase (CoA-acylating) cell culture - 671852, 675222 1.2.1.28 benzaldehyde dehydrogenase (NAD+) cell culture - 676831 1.2.1.3 aldehyde dehydrogenase (NAD+) cell culture - -, 671512, 671542, 672038, 672209 1.2.1.31 L-aminoadipate-semialdehyde dehydrogenase cell culture the activity peaks during growth-phase preceding the onset of antibiotic production, which coincides with a decrease in enzyme activity, and is lower in high cephalosporin-producing strains 654293 1.2.1.31 L-aminoadipate-semialdehyde dehydrogenase cell culture the activity peaks during growth-phase preceding the onset of antibiotic production, which coincides with a decrease in enzyme activity, and is lower in high penicillin-producing strains 654293 1.2.1.32 aminomuconate-semialdehyde dehydrogenase cell culture - -, 672682 1.2.1.36 retinal dehydrogenase cell culture - 677280 1.2.1.39 phenylacetaldehyde dehydrogenase cell culture - 686425 1.2.1.44 cinnamoyl-CoA reductase cell culture - 288230, 288232, 288233 1.2.1.5 aldehyde dehydrogenase [NAD(P)+] cell culture - -, 671511 1.2.1.60 5-carboxymethyl-2-hydroxymuconic-semialdehyde dehydrogenase cell culture - -, 288040, 288297, 288298, 288299, 288300, 288301 1.2.1.60 5-carboxymethyl-2-hydroxymuconic-semialdehyde dehydrogenase cell culture strict dependency for induction of enzyme synthesis of the meta-cleavage pathway by 4-hydroxyphenylacetate -, 288303 1.2.1.61 4-hydroxymuconic-semialdehyde dehydrogenase cell culture - 288304 1.2.1.62 4-formylbenzenesulfonate dehydrogenase cell culture - -, 207945, 207946, 207947 1.2.1.63 6-oxohexanoate dehydrogenase cell culture - -, 288310, 7430 1.2.1.63 6-oxohexanoate dehydrogenase cell culture inducible enzyme synthesis by growth in cyclohexanol -, 246371 1.2.1.63 6-oxohexanoate dehydrogenase cell culture strain TD63 only inducible for enzyme synthesis in the presence of trans-cyclohexan-1,2-diol-containing growth media -, 7430 1.2.1.64 4-hydroxybenzaldehyde dehydrogenase (NAD+) cell culture - -, 288311, 288313 1.2.1.65 salicylaldehyde dehydrogenase cell culture - -, 288316 1.2.1.65 salicylaldehyde dehydrogenase cell culture inducible enzyme synthesis by growth in naphthalene-containing growth media -, 288315 1.2.1.65 salicylaldehyde dehydrogenase cell culture inducible enzyme synthesis only by growth in succinate- and naphthalene-containing media, first inducer for enzyme synthesis of the naphthalene catabolism is salicylaldehyde 288317 1.2.1.65 salicylaldehyde dehydrogenase cell culture induction of enzyme synthesis for naphthalene degradation by salicylate -, 288318, 288319, 288320 1.2.1.65 salicylaldehyde dehydrogenase cell culture induction of enzyme synthesis of the naphthalene degradation pathway by salicylate or 2-hydroxybenzylalcohol 288321 1.2.1.65 salicylaldehyde dehydrogenase cell culture Pseudomonas sp. strains C4, C5 and C6 utilize carbaryl as the sole source of carbon and energy via 1-naphthol, 1,2-dihydroxynaphthalene, benzalpyruvate, salicylaldehyde, salicylate, gentisate and maleylpyruvate, effects on enzyme activities of the carbaryl degradation pathway, overview. Cells grown on salicylate show poor activity for SALDH. Strains C4, C5 and C6 show low overall salicylaldehyde dehydrogenase activity 741793 1.2.1.67 vanillin dehydrogenase cell culture - -, 207983, 207984, 673380 1.2.1.67 vanillin dehydrogenase cell culture grown in yeast extract-vanillin medium -, 207985 1.2.1.67 vanillin dehydrogenase cell culture inactivation of the vdh gene by genetic disruption 207981 1.2.1.67 vanillin dehydrogenase cell culture maximal enzyme synthesis, if cells grown in ferulic acid containing medium -, 207982 1.2.1.67 vanillin dehydrogenase cell culture the cells of strain 3NA grow on ferulic acid, vanillin, vanillic acid, protocatechuic acid, and glucose as carbon sources, best on glucose, followed by protocatechuic acid, and low activity on the other carbon sources -, 741714 1.2.1.68 coniferyl-aldehyde dehydrogenase cell culture induction of enzyme synthesis by growth in eugenol-containing media 288325 1.2.1.70 glutamyl-tRNA reductase cell culture - -, 673633, 674242, 674288, 674450, 676535, 676584 1.2.1.73 sulfoacetaldehyde dehydrogenase cell culture growth with taurine (2-aminoethanesulfonate) as the sole source of combined nitrogen under aerobic conditions -, 675824 1.2.1.88 L-glutamate gamma-semialdehyde dehydrogenase cell culture - 392060, 392061, 392062 1.2.1.96 4-hydroxybenzaldehyde dehydrogenase (NADP+) cell culture - -, 288312, 288314 1.2.3.3 pyruvate oxidase cell culture - -, 390437, 671981, 672023, 675498, 676050 1.2.3.3 pyruvate oxidase cell culture enhanced activity at aerobic growth conditions, reduced activity in the presence of glucose 390436 1.2.3.3 pyruvate oxidase cell culture inducible by pyruvate and iodoacetate 390435 1.2.3.3 pyruvate oxidase cell culture mRNA expression in stationary phase -, 672759 1.2.3.3 pyruvate oxidase cell culture mRNA expression under aerobic conditions and glucose exhaustion -, 655878 1.2.3.6 pyruvate oxidase (CoA-acetylating) cell culture the H2O2 produced by pyruvate oxidase activity inhibits growth of Staphylococcus aureus -, 674287 1.21.99.3 thyroxine 5-deiodinase cell culture astrocytes 644801 1.21.99.3 thyroxine 5-deiodinase cell culture brown adipocytes 644799 1.3.1.45 2'-hydroxyisoflavone reductase cell culture constitutive expression 656914 1.3.1.51 2'-hydroxydaidzein reductase cell culture elicitor-induced 12301 1.3.1.78 arogenate dehydrogenase (NADP+) cell culture - 700819 1.3.1.82 (-)-isopiperitenone reductase cell culture - -, 746552 1.3.3.3 coproporphyrinogen oxidase cell culture - -, 390947, 390952, 390960, 390961, 390962, 390963, 390974, 390975, 390976, 390977, 390979 1.3.3.3 coproporphyrinogen oxidase cell culture copper-responsive expression of mRNA 390954 1.3.3.3 coproporphyrinogen oxidase cell culture expression of oxygen-independent and oxygen-dependent enzymes 390955 1.3.3.4 protoporphyrinogen oxidase cell culture photomixotrophic cells 655018 1.3.3.8 tetrahydroberberine oxidase cell culture - 391075, 391076, 391078, 391079, 726136 1.3.5.1 succinate dehydrogenase cell culture - 688533 1.3.5.2 dihydroorotate dehydrogenase (quinone) cell culture - 391229 1.3.7.12 red chlorophyll catabolite reductase cell culture - 676395 1.3.7.12 red chlorophyll catabolite reductase cell culture in Chlorella, the release of red pigments is correlated with the loss of chlorophyll only if the cells are kept in the dark. These pigments are neither produced in light-grown cells nor in the dark if a source of nitrogen is provided 735913 1.3.7.8 benzoyl-CoA reductase cell culture cells grown on mixtures of non-aromatic acids plus benzoate contain higher amounts of the enzyme as compared to cells grown on the nonaromatic acids alone 288500 1.4.1.14 glutamate synthase (NADH) cell culture - 391471 1.4.3.12 cyclohexylamine oxidase cell culture - 395986 1.4.3.12 cyclohexylamine oxidase cell culture inducible by growth on cyclohexylamine -, 395987, 395988 1.4.3.13 protein-lysine 6-oxidase cell culture - 659751 1.4.3.7 D-glutamate oxidase cell culture - 395286 1.4.9.1 methylamine dehydrogenase (amicyanin) cell culture - 686425 1.4.99.2 taurine dehydrogenase cell culture - 395284, 395285 1.4.99.6 D-arginine dehydrogenase cell culture one methylene blue specific, one 2,6-dichlorophenolindophenol specific enzyme, the methylene blue specific enzyme is only detectable in D-tryptophan grown cells 394974, 394975 1.5.1.3 dihydrofolate reductase cell culture evaluation of cytotoxicity of compounds 2,4-diamino-5-propyl-6-arylthio-7H-pyrrolo[2,3-d]pyrimidine and 2,4-diamino-5-isopropyl-6-arylthio-7H-pyrrolo[2,3-d]pyrimidine against 17 different tumor cell lines. GI values of compound 2,4-diamino-5-propyl-6-arylthio-7H-pyrrolo[2,3-d]pyrimidine are below 100 nM 688285 1.5.3.10 dimethylglycine oxidase cell culture - -, 394957, 394961 1.5.3.12 dihydrobenzophenanthridine oxidase cell culture - 13612, 742175 1.5.3.12 dihydrobenzophenanthridine oxidase cell culture elicited cell suspension 13613, 13615, 13616 1.5.3.12 dihydrobenzophenanthridine oxidase cell culture elicited cells 13614 1.5.3.12 dihydrobenzophenanthridine oxidase cell culture elicited tissues 13615 1.5.8.2 trimethylamine dehydrogenase cell culture - -, 393878, 393879, 393881, 393884, 393885, 393887, 393888, 393889, 393890, 393891, 393892, 393893, 393894, 393895, 393896, 393897, 393898, 393899, 393900, 393901, 393902, 393903, 393904, 393905, 393908, 393909, 393911, 393913, 393914, 393915, 393916, 393917, 393918, 393919, 393920, 393921, 393922, 393923, 393924, 393925 1.6.5.2 NAD(P)H dehydrogenase (quinone) cell culture H2O2-scavenging and growth of wild-type, overexpressing, and knockout cells, overview -, 680629 1.6.5.4 monodehydroascorbate reductase (NADH) cell culture - 392728 1.7.1.3 nitrate reductase (NADPH) cell culture free-living cells, yeast extract/mannitol medium 655625 1.7.1.4 nitrite reductase [NAD(P)H] cell culture free-living cells, yeast extract/mannitol medium 655625 1.7.1.6 azobenzene reductase cell culture maximal enzyme production of 0.39 U/ml in mutant strain B1 at pH 6.5, 35°C, 2% D-glucose, 1% sodium nitrate, and 2% of inoculum concentration 741799 1.7.3.3 factor-independent urate hydroxylase cell culture strain CGMCC 2.120 is used as source of uricase gene 695795 1.8.1.15 mycothione reductase cell culture gene is actively transcribed during logarithmic growth 658947 1.8.1.4 dihydrolipoyl dehydrogenase cell culture - 659866 1.8.1.8 protein-disulfide reductase cell culture the enzyme is at a low level in log-phase cells but increases up to 10fold early in the stationary phase and has a similar specific activity in both the mother cell and the forespore compartment. The enzyme activity falls only slowly during spore germination and outgrowth 680424 1.8.7.1 assimilatory sulfite reductase (ferredoxin) cell culture - 437707 1.8.98.4 coenzyme F420:CoB-CoM heterodisulfide,ferredoxin reductase cell culture coculture of Methanosarcina barkeri with Geobacter metallireducens, transcriptome analysis, overview. Out of the 3809 predicted protein-coding genes in the Methanosarcina barkeri MS genome, 1912 and 1909 genes have expression levels that are higher than the median in DIET-grown cells, respectively -, 764762 1.8.98.5 H2:CoB-CoM heterodisulfide,ferredoxin reductase cell culture coculture of Methanosarcina barkeri with Pelobacter carbinolicus, transcriptome analysis, overview. Out of the 3809 predicted protein-coding genes in the Methanosarcina barkeri MS genome, 1912 and 1909 genes have expression levels that are higher than the median in HIT-grown cells, respectively -, 764762 1.8.98.5 H2:CoB-CoM heterodisulfide,ferredoxin reductase cell culture Methanonatronarchaeum thermophilum strain AMET1 is able to grow on all tested substrates (methanol, trimethylamine (TMA), dimethylamine (DMA), monomethylamine (MMA)) in combination with formate or molecular hydrogen -, 764660 1.8.98.6 formate:CoB-CoM heterodisulfide,ferredoxin reductase cell culture Methanonatronarchaeum thermophilum strain AMET1 is able to grow on all tested substrates (methanol, trimethylamine (TMA), dimethylamine (DMA), monomethylamine (MMA)) in combination with formate or molecular hydrogen. Growth parameter during methanogenesis from methylated C1-compounds and formate, overview -, 764660 1.8.99.B2 tetrathionate reductase cell culture enzyme activity measured using ferricyanide as the electron acceptor is detected in cell extracts of Astrain ATCC 23270 grown on tetrathionate or sulfur, but no activity is detected in ferrous iron-grown cells 741675 2.1.1.100 protein-S-isoprenylcysteine O-methyltransferase cell culture insulin-secreting 485036 2.1.1.105 N-benzoyl-4-hydroxyanthranilate 4-O-methyltransferase cell culture - 485081 2.1.1.11 magnesium protoporphyrin IX methyltransferase cell culture - 684982, 687761 2.1.1.113 site-specific DNA-methyltransferase (cytosine-N4-specific) cell culture - 686788 2.1.1.115 (RS)-1-benzyl-1,2,3,4-tetrahydroisoquinoline N-methyltransferase cell culture - 15190 2.1.1.116 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase cell culture - 15191 2.1.1.117 (S)-scoulerine 9-O-methyltransferase cell culture - 15196 2.1.1.117 (S)-scoulerine 9-O-methyltransferase cell culture activity of high berberine-producing cells is higher than that of non-selected cells 15192 2.1.1.120 12-hydroxydihydrochelirubine 12-O-methyltransferase cell culture - 7393 2.1.1.122 (S)-tetrahydroprotoberberine N-methyltransferase cell culture elicitor treatment 674802 2.1.1.128 (RS)-norcoclaurine 6-O-methyltransferase cell culture - 15229, 15232 2.1.1.14 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase cell culture - 441337 2.1.1.140 (S)-coclaurine-N-methyltransferase cell culture - 15231, 288659, 659190, 687805 2.1.1.154 isoliquiritigenin 2'-O-methyltransferase cell culture - 654325, 657009 2.1.1.154 isoliquiritigenin 2'-O-methyltransferase cell culture rapid and transient increase in extractable activities after treatment with yeast extract 485511 2.1.1.170 16S rRNA (guanine527-N7)-methyltransferase cell culture activity of rsmGp slightly increases by fourfold when cells are grown in minimal media supplemented with glycerol instead of glucose 721042 2.1.1.233 [phosphatase 2A protein]-leucine-carboxy methyltransferase cell culture - -, 750614 2.1.1.240 trans-resveratrol di-O-methyltransferase cell culture - 742957 2.1.1.246 [methyl-Co(III) methanol-specific corrinoid protein]:coenzyme M methyltransferase cell culture cell growth on methanol, or trimethylamine, both with tetracycline 698630 2.1.1.246 [methyl-Co(III) methanol-specific corrinoid protein]:coenzyme M methyltransferase cell culture the isozymes MT2-A and MT2-M are differentially expressed depending upon the substrate available for growth 717753 2.1.1.269 dimethylsulfoniopropionate demethylase cell culture - -, 757585 2.1.1.280 selenocysteine Se-methyltransferase cell culture cell cultures contain the maximal level of selenocysteine methyltransferase under conditions of exponential growth. Stationary phase cells possess a much lower amount 692948 2.1.1.296 methyltransferase cap2 cell culture cultured lens cell 756633 2.1.1.37 DNA (cytosine-5-)-methyltransferase cell culture - 703330 2.1.1.53 putrescine N-methyltransferase cell culture root culture 662536, 663228 2.1.1.63 methylated-DNA-[protein]-cysteine S-methyltransferase cell culture activity in cells adapted by exposure to N-methyl-N1-nitroguanidine, no activity in extracts of nonadapted cells 485487 2.1.1.65 licodione 2'-O-methyltransferase cell culture - 485510, 485511 2.1.1.69 5-hydroxyfuranocoumarin 5-O-methyltransferase cell culture - 485550 2.1.1.70 8-hydroxyfuranocoumarin 8-O-methyltransferase cell culture - 485550 2.1.1.72 site-specific DNA-methyltransferase (adenine-specific) cell culture growth temperature for strain 14P is 55°C -, 684558 2.1.1.79 cyclopropane-fatty-acyl-phospholipid synthase cell culture increase in expression of CFA synthase at early stationary phase is due to the alternative sigma factor RpoS 659895 2.1.2.1 glycine hydroxymethyltransferase cell culture optimum growth temperature of 70-75°C -, 719472 2.1.3.2 aspartate carbamoyltransferase cell culture - 1734 2.1.3.2 aspartate carbamoyltransferase cell culture 165-23 cell line 485864 2.1.3.2 aspartate carbamoyltransferase cell culture fibroblast cell line MA-134 485845 2.1.3.2 aspartate carbamoyltransferase cell culture simian virus 40 transformed syrian hamster cell line 485845, 485852 2.2.1.10 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate synthase cell culture optimal growth temperature is 85°C 692386 2.2.1.6 acetolactate synthase cell culture - 699740 2.2.1.7 1-deoxy-D-xylulose-5-phosphate synthase cell culture - 676738 2.3.1.115 isoflavone-7-O-beta-glucoside 6''-O-malonyltransferase cell culture - 486143, 486144 2.3.1.116 flavonol-3-O-beta-glucoside O-malonyltransferase cell culture - 486143, 486144 2.3.1.138 putrescine N-hydroxycinnamoyltransferase cell culture - -, 486314 2.3.1.150 Salutaridinol 7-O-acetyltransferase cell culture - 16324 2.3.1.151 2,3',4,6-tetrahydroxybenzophenone synthase cell culture - 16326, 660170, 706131 2.3.1.169 CO-methylating acetyl-CoA synthase cell culture cultures are grown on methanol, acetate, or CO 719744 2.3.1.177 3,5-dihydroxybiphenyl synthase cell culture - 718719, 720794 2.3.1.177 3,5-dihydroxybiphenyl synthase cell culture yeast-extract-treated 663182 2.3.1.19 phosphate butyryltransferase cell culture activity is maximal in the late exponential growth phase. The branched amino acids isoleucine and valine activate Ptb expression. ActBm, a sigma54 regulator from Bacillus megaterium whose gene is situated upstream from the ptb gene, activates its expression 486476 2.3.1.191 UDP-3-O-(3-hydroxyacyl)glucosamine N-acyltransferase cell culture growth temperatures of 18°C to 37°C -, 720944 2.3.1.208 4-hydroxycoumarin synthase cell culture elicitor-treated 706131, 706258 2.3.1.208 4-hydroxycoumarin synthase cell culture elicitor-treated cell cultures 721091 2.3.1.23 1-acylglycerophosphocholine O-acyltransferase cell culture endothelial cells 486644 2.3.1.250 [Wnt protein] O-palmitoleoyl transferase cell culture - 733769 2.3.1.304 poly[(R)-3-hydroxyalkanoate] polymerase cell culture highest P(3HB) production of 2.38 g/l at 68 wt% attained with crude palm kernel oil as the sole carbon source -, 721109 2.3.1.37 5-aminolevulinate synthase cell culture - 486815 2.3.1.37 5-aminolevulinate synthase cell culture high activity in cells grown anaerobically in defined medium, low in cells grown in an iron-deficient medium and in cells grown aerobically 486810 2.3.1.42 glycerone-phosphate O-acyltransferase cell culture CHO-K1 cell line 659671 2.3.1.48 histone acetyltransferase cell culture - 703857 2.3.1.48 histone acetyltransferase cell culture african green monkey kidney cell line CV-1 infected with SV40 487076 2.3.1.48 histone acetyltransferase cell culture H4 acetylation at origins by HBO1 is cell-cycle regulated, with maximal activity at the G1/S transition 705721 2.3.1.50 serine C-palmitoyltransferase cell culture leukemia Molt-4 cells 487173, 487174 2.3.1.50 serine C-palmitoyltransferase cell culture neuroblastoma cell line 487174 2.3.1.51 1-acylglycerol-3-phosphate O-acyltransferase cell culture - 687624, 687813 2.3.1.54 formate C-acetyltransferase cell culture formate accumulation inside anaerobically adapted Chlamydomonas reinhardtii CC-277 cells measured 686477 2.3.1.54 formate C-acetyltransferase cell culture microaerobic cultures, glucose-limited chemostat cultures, different strains, growth rates, overview 672714 2.3.1.6 choline O-acetyltransferase cell culture - 702964 2.3.1.75 long-chain-alcohol O-fatty-acyltransferase cell culture cells grown on phytol as the sole carbon source under limiting nitrogen and/or phosphorous conditions 674328 2.3.1.78 heparan-alpha-glucosaminide N-acetyltransferase cell culture from primary skin fibroblasts, wild-type SF4528 and KD8, and MPS IIIC mutants thereof 661443 2.3.2.2 gamma-glutamyltransferase cell culture - -, 487901, 487935, 487950, 487953, 487958, 487960 2.3.2.2 gamma-glutamyltransferase cell culture carbon stressed cultures -, 754814 2.3.2.2 gamma-glutamyltransferase cell culture in wild-type the enzyme is synthesized only during the mid-stationary phase -, 658567 2.3.3.15 sulfoacetaldehyde acetyltransferase cell culture - 698627 2.3.3.15 sulfoacetaldehyde acetyltransferase cell culture ISM strain 698626 2.4.1.10 levansucrase cell culture enzyme production is performed by free and differently immobilized cells, maximum yield with 18.47 U/ml is achieved after 48 h in batch shaken culture of the free cells, but immobilized culture technique allows the achievement of relatively high enzyme activities of 5.5-10.75 U/ml with successful repeated utilization of the immobilized cells 701566 2.4.1.115 anthocyanidin 3-O-glucosyltransferase cell culture - 656613, 657091, 657117 2.4.1.12 cellulose synthase (UDP-forming) cell culture - 489072 2.4.1.155 alpha-1,6-mannosyl-glycoprotein 6-beta-N-acetylglucosaminyltransferase cell culture - 488370 2.4.1.16 chitin synthase cell culture Chs3p and Chs4p appear to co-localize at the neck region of the budding yeast 687914 2.4.1.16 chitin synthase cell culture growth of wild-type and enzyme mutant cells, overview -, 735794 2.4.1.173 sterol 3beta-glucosyltransferase cell culture - 722093 2.4.1.173 sterol 3beta-glucosyltransferase cell culture crude extracts from culture 637769 2.4.1.173 sterol 3beta-glucosyltransferase cell culture leaf cell culture 637770 2.4.1.173 sterol 3beta-glucosyltransferase cell culture mycoplasma strain M3C IV 637766, 637771 2.4.1.173 sterol 3beta-glucosyltransferase cell culture strain F and J activity only in diploid cells 637767 2.4.1.174 glucuronylgalactosylproteoglycan 4-beta-N-acetylgalactosaminyltransferase cell culture - 723586 2.4.1.175 glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase cell culture - 723586 2.4.1.177 cinnamate beta-D-glucosyltransferase cell culture maximum enzymic activity in pre-logarithmic to logarithmic growth phase 660116 2.4.1.18 1,4-alpha-glucan branching enzyme cell culture - 706685 2.4.1.2 dextrin dextranase cell culture optimization of the fermentation medium for DDase production by Gluconaobacter oxydans M5, detailed overview. The optimum medium consisted of glucose 17.670 g/l, maltobiose 30 g/l, tryptone 12.198 g/l, yeast extract 13.528 g/l, ammonium nitrate 15 g/l, copper sulfate 0.01 g/l, zinc sulfate 0.01 g/l, and sodium chloride 0.009 g/l at pH 6.0 and 30°C -, 718532 2.4.1.210 limonoid glucosyltransferase cell culture suspension 660114 2.4.1.216 trehalose 6-phosphate phosphorylase cell culture - -, 395984, 395985 2.4.1.218 hydroquinone glucosyltransferase cell culture - 393093, 393094 2.4.1.34 1,3-beta-glucan synthase cell culture budding and filamentous 489064 2.4.1.5 dextransucrase cell culture dextransucrase is produced in a fermentation medium using cashew apple, Anacardium occidentale, juice as substrate, method evaluation, overview -, 703787 2.4.1.5 dextransucrase cell culture optimization of culture conditions for dextransucrase production, optimum in a shaking flask culture at pH 5.4, 25°C, overview 704907 2.4.1.5 dextransucrase cell culture the dextransucrase from the organism shows maximum production with addition of 0.005% CaCl2, production method optimization, overview -, 703991 2.4.1.5 dextransucrase cell culture the enzyme production and activity is highest at pH 6.5 and 25°C, high stability at room temperature of 30°C, condition evaluation, overview -, 719585 2.4.1.83 dolichyl-phosphate beta-D-mannosyltransferase cell culture - 488483, 489440 2.4.1.83 dolichyl-phosphate beta-D-mannosyltransferase cell culture encysting 489440 2.4.1.97 1,3-beta-D-glucan phosphorylase cell culture - 639635 2.4.2.7 adenine phosphoribosyltransferase cell culture - 638152 2.5.1.18 glutathione transferase cell culture - 691484 2.5.1.2 thiamine pyridinylase cell culture - -, 636848, 636849, 636850, 636852, 636853, 636858, 636859 2.5.1.2 thiamine pyridinylase cell culture addition of yeast extract and Ca2+ in the medium increases production of thiaminase, addition of glutamate or brain heart infusion decreases production -, 636857 2.5.1.22 spermine synthase cell culture - 721528 2.5.1.23 sym-norspermidine synthase cell culture - 638427 2.5.1.36 trihydroxypterocarpan dimethylallyltransferase cell culture - 489902, 489903 2.5.1.41 phosphoglycerol geranylgeranyltransferase cell culture from an early stationary phase culture -, 638437 2.5.1.42 geranylgeranylglycerol-phosphate geranylgeranyltransferase cell culture from an early stationary stage cell culture -, 638437 2.5.1.46 deoxyhypusine synthase cell culture DHS is ubiquitously expressed in primary mouse cells and murine cell lines 722424 2.5.1.54 3-deoxy-7-phosphoheptulonate synthase cell culture - 639755, 639765, 639773, 639780 2.5.1.59 protein geranylgeranyltransferase type I cell culture the expression of GGTIalpha in cultured hippocampal neurons gradually increases and peaks at approximately 8 days in vitro 739114 2.5.1.59 protein geranylgeranyltransferase type I cell culture the expression of GGTIbeta in cultured hippocampal neurons gradually increases and peaks at approximately 8 days in vitro 739114 2.6.1.19 4-aminobutyrate-2-oxoglutarate transaminase cell culture - 698627 2.6.1.19 4-aminobutyrate-2-oxoglutarate transaminase cell culture growth at temperatures of up to 50°C -, 759234 2.6.1.5 tyrosine transaminase cell culture fetal hepatocyte primary cultures, synergistic induction of enzyme in presence of glucagon and dexamethasone 658409 2.6.1.76 diaminobutyrate-2-oxoglutarate transaminase cell culture growth at temperatures of up to 50°C -, 759234 2.6.1.78 aspartate-prephenate aminotransferase cell culture rosmarinic acid-producing cells 657636 2.6.1.79 glutamate-prephenate aminotransferase cell culture - 659858 2.6.1.79 glutamate-prephenate aminotransferase cell culture stable cell line ANS-1 657635 2.7.1.1 hexokinase cell culture glucokinase is continuously expressed during the all growth stages, and the peak value for glucokinase activity occurrs in the stationary growth phase before spores form 671492 2.7.1.105 6-phosphofructo-2-kinase cell culture - 661569 2.7.1.108 dolichol kinase cell culture - 739079 2.7.1.108 dolichol kinase cell culture temperature-sensitive Saccharomyces cerevisiae mutants, impaired in dolichol kinase (Sec59p) or dolichyl phosphate mannose synthase (Dpm1p) activity have an aberrant cell wall composition and ultrastructure -, 677274 2.7.1.12 gluconokinase cell culture - 696574 2.7.1.127 inositol-trisphosphate 3-kinase cell culture - 663233 2.7.1.127 inositol-trisphosphate 3-kinase cell culture cloning and expression in Escherichia coli 687650 2.7.1.127 inositol-trisphosphate 3-kinase cell culture Enzymes and their fragments expressed in Escherichia coli. 687650 2.7.1.127 inositol-trisphosphate 3-kinase cell culture Enzymes expressed in Escherichia coli 687650 2.7.1.137 phosphatidylinositol 3-kinase cell culture - 709772 2.7.1.137 phosphatidylinositol 3-kinase cell culture 3T3-L1 cells 640926 2.7.1.137 phosphatidylinositol 3-kinase cell culture a N-terminal truncated form is found a certain hematopoietic cell line 640947 2.7.1.137 phosphatidylinositol 3-kinase cell culture B lymphoma cell line 640869 2.7.1.137 phosphatidylinositol 3-kinase cell culture BAF3 cells and primary murine megakaryocyte 640902 2.7.1.137 phosphatidylinositol 3-kinase cell culture breast cancer cells 640885 2.7.1.137 phosphatidylinositol 3-kinase cell culture carcinoma-derived A431 cells -, 640861 2.7.1.137 phosphatidylinositol 3-kinase cell culture CHO cells 640925 2.7.1.137 phosphatidylinositol 3-kinase cell culture Fao hepatoma cells 640913 2.7.1.137 phosphatidylinositol 3-kinase cell culture Friend erythroleukemia cells 640874 2.7.1.137 phosphatidylinositol 3-kinase cell culture hepatocyte primary culture 640883 2.7.1.137 phosphatidylinositol 3-kinase cell culture HL-60 cells 640886 2.7.1.137 phosphatidylinositol 3-kinase cell culture KRC-7 hepatoma cells 640858 2.7.1.137 phosphatidylinositol 3-kinase cell culture of astrocytes from cerebral cortices -, 708599 2.7.1.137 phosphatidylinositol 3-kinase cell culture osteosarcoma Saos-2 cells 640866 2.7.1.137 phosphatidylinositol 3-kinase cell culture osteosarcoma sensitive and multidrug-resistant Saos-2 cells 640929 2.7.1.137 phosphatidylinositol 3-kinase cell culture PC 12 cells 640853 2.7.1.137 phosphatidylinositol 3-kinase cell culture PC12 cells 640853 2.7.1.137 phosphatidylinositol 3-kinase cell culture Swiss 3T3 fibroblast 640870 2.7.1.137 phosphatidylinositol 3-kinase cell culture T cell line CTLL-2 640889 2.7.1.137 phosphatidylinositol 3-kinase cell culture U937 monocyte 640862 2.7.1.137 phosphatidylinositol 3-kinase cell culture U937 monocytes 640862 2.7.1.148 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase cell culture cloned in Escherichia coli 686699 2.7.1.149 1-phosphatidylinositol-5-phosphate 4-kinase cell culture DT40 cell from chicken, that expresses active PIPkin IIbetA tagged at the C-terminus of Hef, a protein component of the Fanconi anemia-related tumor-suppressor complex. 686033 2.7.1.151 inositol-polyphosphate multikinase cell culture - 663233 2.7.1.156 adenosylcobinamide kinase cell culture - 674340 2.7.1.156 adenosylcobinamide kinase cell culture strain LT2 687405 2.7.1.158 inositol-pentakisphosphate 2-kinase cell culture expression of Solanum tuberosum protein in Escherichia coli 686761 2.7.1.162 N-acetylhexosamine 1-kinase cell culture strain JCM1217 -, 677700 2.7.1.163 hygromycin B 4-O-kinase cell culture - 689502 2.7.1.163 hygromycin B 4-O-kinase cell culture Agrobacterium tumefaciens strain LBA4404 harbors the binary vector plasmid pIG121Hm containing hpt gene from Escherichia coli 689450 2.7.1.163 hygromycin B 4-O-kinase cell culture bacterial strain SK1044 688322 2.7.1.163 hygromycin B 4-O-kinase cell culture strain B834 671134 2.7.1.163 hygromycin B 4-O-kinase cell culture strain BL21 671134 2.7.1.163 hygromycin B 4-O-kinase cell culture strain BL23 (DE3) 685684 2.7.1.163 hygromycin B 4-O-kinase cell culture strain EHA 101 is used for transformation 689511 2.7.1.163 hygromycin B 4-O-kinase cell culture strain T23 685633 2.7.1.163 hygromycin B 4-O-kinase cell culture strains W677, BE783, BE827, DH1, BE1065, BE1092, and BE1098 are used 684479 2.7.1.17 xylulokinase cell culture Pseudomonas strain S12 containing xylose isomerase gene and xylulokinase gene from Escherichia coli strain DH5 alpha 690541 2.7.1.2 glucokinase cell culture glk is a nonessential gene that is constitutively expressed during logarithmic growth and has slightly reduced expression in the stationary phase 680430 2.7.1.20 adenosine kinase cell culture - 661815, 662358 2.7.1.20 adenosine kinase cell culture enzyme activity decreases by 5.5fold in senescent cell cultures, 1.5-3.5fold increased activity of both isozymes during cell wall synthesis 661815 2.7.1.23 NAD+ kinase cell culture grown heterotrophically in darkness at pH 3.5 in the presence of lactate as sole carbon source. Treatment with AlCl3 slows down the culture growth and suppresses the peak of NAD+ kinase activity, which characterizes the beginning of the exponential phase of growth of the control cultures, possible explanations -, 641188 2.7.1.23 NAD+ kinase cell culture two peaks of calmodulin-dependent NAD+ kinase activity occur at times when the cells are not actively dividing: the first one, a few hours after medium inoculation and the second one, at the end of the exponential growth phase 641199 2.7.1.32 choline kinase cell culture clone NF-54 671383 2.7.1.32 choline kinase cell culture strainn 427 is used for isolation of RNA 685011 2.7.1.4 fructokinase cell culture - -, 640234 2.7.1.40 pyruvate kinase cell culture - -, 3241, 641516, 641525, 641556, 641557, 641571, 641572, 641575, 641576, 641577, 641578, 641579, 641582, 641583, 641584, 641585, 641586, 641587, 641588, 641589, 641590, 641596, 641597, 641598, 641599, 641600 2.7.1.40 pyruvate kinase cell culture aggregating 641522 2.7.1.40 pyruvate kinase cell culture hyphal and yeast 641581 2.7.1.40 pyruvate kinase cell culture vegetative -, 641549 2.7.1.64 inositol 3-kinase cell culture - 636603, 636605 2.7.1.68 1-phosphatidylinositol-4-phosphate 5-kinase cell culture Escherichia coli BL21 cell is used for cloning and expression 689477 2.7.1.7 mannokinase cell culture grown on D-mannose or beta-D-mannan 641893 2.7.1.78 polynucleotide 5'-hydroxyl-kinase cell culture chinese hamster lung cells 642004 2.7.1.78 polynucleotide 5'-hydroxyl-kinase cell culture HeLa cells 642004, 642021 2.7.1.86 NADH kinase cell culture - 657853 2.7.1.90 diphosphate-fructose-6-phosphate 1-phosphotransferase cell culture - -, 642104, 642105 2.7.1.91 sphingosine kinase cell culture melanoma B16 cells, variant F10, Swiss 3T3, balb/c 3T3 clone A31 492159 2.7.10.1 receptor protein-tyrosine kinase cell culture - 490320, 490371, 490636 2.7.10.1 receptor protein-tyrosine kinase cell culture acute myelogenic cell line 490540 2.7.10.1 receptor protein-tyrosine kinase cell culture amplification of the c-erb-B-2 gene in gastric cancer cell line MKN-7 490105 2.7.10.1 receptor protein-tyrosine kinase cell culture breast cancer cells SC-3 490264 2.7.10.1 receptor protein-tyrosine kinase cell culture breast carcinoma cells 490581 2.7.10.1 receptor protein-tyrosine kinase cell culture carcinoma cell line 490317 2.7.10.1 receptor protein-tyrosine kinase cell culture epidermoid carcinoma cell line A431 490487 2.7.10.1 receptor protein-tyrosine kinase cell culture epithelial cell line B5/589 490322 2.7.10.1 receptor protein-tyrosine kinase cell culture epithelial ovarian cancer cell line SKOV-3 490582 2.7.10.1 receptor protein-tyrosine kinase cell culture expressed in populations enriched for stem cells and primitive uncommitted progenitors 490512 2.7.10.1 receptor protein-tyrosine kinase cell culture expression of the tie gene is restricted in some cell lines: large amounts of tie mRNA are detected in endothelial cell lines and in some myeloid leukemia cell lines with erythroid and megakaryoblastoid characteristics 490391 2.7.10.1 receptor protein-tyrosine kinase cell culture gastric cancer cell line KATO-III 490561 2.7.10.1 receptor protein-tyrosine kinase cell culture gene is amplified in stomach cancer-derived cell line, KATO-III 490289, 490292 2.7.10.1 receptor protein-tyrosine kinase cell culture glioblastoma cell line SW-1088 490175 2.7.10.1 receptor protein-tyrosine kinase cell culture HeLa cells 490363 2.7.10.1 receptor protein-tyrosine kinase cell culture hepatoma HepG2 490569 2.7.10.1 receptor protein-tyrosine kinase cell culture induced upon neuronal differentiation of P19EC cells 490660 2.7.10.1 receptor protein-tyrosine kinase cell culture K-562 leukemia cells 490317 2.7.10.1 receptor protein-tyrosine kinase cell culture K562 erythroleukemia cells 490317 2.7.10.1 receptor protein-tyrosine kinase cell culture malignant cell lines 490495 2.7.10.1 receptor protein-tyrosine kinase cell culture mammary gland-derived epithelial cell line 490506 2.7.10.1 receptor protein-tyrosine kinase cell culture mast cell leukemia cell line HMC-1 490208 2.7.10.1 receptor protein-tyrosine kinase cell culture NIH 3T3 cells 490260 2.7.10.1 receptor protein-tyrosine kinase cell culture PC12 cells 490392 2.7.10.1 receptor protein-tyrosine kinase cell culture pre-B cell line LK63 490365, 490366 2.7.10.1 receptor protein-tyrosine kinase cell culture premegakaryocytopoietic cell lines CMK11-5 and Dami 490502 2.7.10.1 receptor protein-tyrosine kinase cell culture prominent expression in the embryonal carcinoma cell line NT2/D1 490567 2.7.10.1 receptor protein-tyrosine kinase cell culture several breast carcinoma cell lines 490599 2.7.10.1 receptor protein-tyrosine kinase cell culture several human tumor cell lines derived from neuroectoderm including primitive neuroectodermal tumor, small cell lung carcinoma, and neuroblastoma express NET transcripts 490499 2.7.10.1 receptor protein-tyrosine kinase cell culture trkE transcript is expressed at low levels by PC12 cells 490580 2.7.10.1 receptor protein-tyrosine kinase cell culture Wilms' tumor cell line, a retinoblastoma cell line, and a nondifferentiated teratocarcinoma cell line express FLT4. Differentiated teratocarcinoma cells are negative 490396 2.7.10.2 non-specific protein-tyrosine kinase cell culture - 490438, 661540 2.7.10.2 non-specific protein-tyrosine kinase cell culture astrocytes 490665 2.7.10.2 non-specific protein-tyrosine kinase cell culture BALB/c 3T3 cells 490675 2.7.10.2 non-specific protein-tyrosine kinase cell culture basophilic leukemia cell line KU812 490455 2.7.10.2 non-specific protein-tyrosine kinase cell culture cell line K562 490059 2.7.10.2 non-specific protein-tyrosine kinase cell culture cell lines of breast and colon origin 490442 2.7.10.2 non-specific protein-tyrosine kinase cell culture cells derived from multiple hematopoietic lineages 490452 2.7.10.2 non-specific protein-tyrosine kinase cell culture enzyme from NIH 3T3 cells is expressed in COS-7 cells 491974 2.7.10.2 non-specific protein-tyrosine kinase cell culture enzyme is expressed constitutively at a low level in normal T-cells and at an elevated level in the LSTRA and Thy19 Moloney murine leukemia virus-induced thymoma cell lines 490123, 490124 2.7.10.2 non-specific protein-tyrosine kinase cell culture expressed at low levels in human umbilical vein endothelial cells, human aortic smooth muscle cells, A549, and DLD-1 human colon adenocarcinoma cells 490472 2.7.10.2 non-specific protein-tyrosine kinase cell culture expressed in all stages of the B lineage and in myeloid cells 490410 2.7.10.2 non-specific protein-tyrosine kinase cell culture expressed in myeloid and B-lymphoid lineage cells 490355 2.7.10.2 non-specific protein-tyrosine kinase cell culture expression of matk mRNA is predominantly found in cells of megakaryocytic lineage 490431 2.7.10.2 non-specific protein-tyrosine kinase cell culture HeLa cells 490673, 532737 2.7.10.2 non-specific protein-tyrosine kinase cell culture hemopoietic cell line FDC-P1 490464, 490626 2.7.10.2 non-specific protein-tyrosine kinase cell culture hepatoma cell line Hep3B 490444 2.7.10.2 non-specific protein-tyrosine kinase cell culture highly expressed in many hematopoietic cell lines 490434 2.7.10.2 non-specific protein-tyrosine kinase cell culture K-562 leukemia cells 490320 2.7.10.2 non-specific protein-tyrosine kinase cell culture lymphoid cells 490673 2.7.10.2 non-specific protein-tyrosine kinase cell culture lymphoma cell line 490118 2.7.10.2 non-specific protein-tyrosine kinase cell culture megakaryoblastic cell line UT-7 490433 2.7.10.2 non-specific protein-tyrosine kinase cell culture melanoma or fibroblast cell lines 490632 2.7.10.2 non-specific protein-tyrosine kinase cell culture natural-killer-like cell line 490468 2.7.10.2 non-specific protein-tyrosine kinase cell culture neuroblastoma cells 490673 2.7.10.2 non-specific protein-tyrosine kinase cell culture neurons 490665 2.7.10.2 non-specific protein-tyrosine kinase cell culture ovarian cancer cells 491944 2.7.10.2 non-specific protein-tyrosine kinase cell culture pituitary tumor cell line GH3 491962 2.7.10.2 non-specific protein-tyrosine kinase cell culture predominantly expressed within the T cell lineage 490437 2.7.10.2 non-specific protein-tyrosine kinase cell culture primary ascites cells 491944 2.7.10.2 non-specific protein-tyrosine kinase cell culture RBL-2H3 cells 490646 2.7.10.2 non-specific protein-tyrosine kinase cell culture src-related gene is expressed in normal macrophages and in cell lines representing both the myeloid and lymphoid B-cell lineages 490151 2.7.10.2 non-specific protein-tyrosine kinase cell culture T cells and some myeloid cell lines 490435 2.7.10.2 non-specific protein-tyrosine kinase cell culture various myeloid cell lines 490433 2.7.10.2 non-specific protein-tyrosine kinase cell culture widely expressed in hematopoietic and non-hematopoietic cell lines 490378 2.7.11.1 non-specific serine/threonine protein kinase cell culture - 673856 2.7.11.1 non-specific serine/threonine protein kinase cell culture cell lines MCF-7 and WI38 491059, 491134 2.7.11.1 non-specific serine/threonine protein kinase cell culture Con8.hd6 rat mammary tumor cell line 491180 2.7.11.1 non-specific serine/threonine protein kinase cell culture HeLa cells 491162, 491246, 491331, 532661 2.7.11.1 non-specific serine/threonine protein kinase cell culture hepatoma cell line 491055 2.7.11.1 non-specific serine/threonine protein kinase cell culture HepG2 cell 490479 2.7.11.1 non-specific serine/threonine protein kinase cell culture ovarian carcinoma cell lines 491133 2.7.11.1 non-specific serine/threonine protein kinase cell culture rat BUB1 mRNA accumulation correlates with the proliferation status of cells in culture 532759 2.7.11.1 non-specific serine/threonine protein kinase cell culture SKOV3 ovarian carcinoma cell 491029 2.7.11.10 IkappaB kinase cell culture cell line KG-1 532724 2.7.11.11 cAMP-dependent protein kinase cell culture - 490962 2.7.11.11 cAMP-dependent protein kinase cell culture myoblast L6 cell line 490995 2.7.11.11 cAMP-dependent protein kinase cell culture ovary cell line 490962 2.7.11.13 protein kinase C cell culture - 661112 2.7.11.13 protein kinase C cell culture epidermoid carcinoma line A431 491130 2.7.11.13 protein kinase C cell culture fibrosarcoma cell line 491114 2.7.11.13 protein kinase C cell culture insulin-secreting cell line RINm5F 491149 2.7.11.13 protein kinase C cell culture NIH/hIR/rIRS1cells 675999 2.7.11.13 protein kinase C cell culture PR-17 cells and wild-type HL-60 cells 491109 2.7.11.13 protein kinase C cell culture primary 676029 2.7.11.13 protein kinase C cell culture several hemopoietic tumor lines 491171 2.7.11.13 protein kinase C cell culture Swiss 3T3 fibroblasts 491115 2.7.11.13 protein kinase C cell culture undifferentiated mouse embryonal carcinoma cell line P19, NIH 3T3 cells 491197 2.7.11.15 beta-adrenergic-receptor kinase cell culture - 671267 2.7.11.16 G-protein-coupled receptor kinase cell culture spermatogonia cell line GC-1 spg 491135 2.7.11.17 Ca2+/calmodulin-dependent protein kinase cell culture Jurkat cell 491288 2.7.11.19 phosphorylase kinase cell culture HeLa cells 491246 2.7.11.2 [pyruvate dehydrogenase (acetyl-transferring)] kinase cell culture - 661695 2.7.11.21 polo kinase cell culture - 661534 2.7.11.22 cyclin-dependent kinase cell culture only expressed during vegetative cell growth 491564 2.7.11.22 cyclin-dependent kinase cell culture primary midbrain mesencephalonic cells 662686 2.7.11.23 [RNA-polymerase]-subunit kinase cell culture - 490907, 640975, 640976, 640978, 640979, 640980 2.7.11.24 mitogen-activated protein kinase cell culture - 491805, 662107 2.7.11.24 mitogen-activated protein kinase cell culture ERK is associated with gamma-tubulin on microtubules in proliferating cells 741070 2.7.11.24 mitogen-activated protein kinase cell culture expressed at low levels in G1 phase but at higher levels in S and G2 phases of the cell cycle 491798 2.7.11.24 mitogen-activated protein kinase cell culture hepatoma cell line 491741 2.7.11.24 mitogen-activated protein kinase cell culture inflammatory cell lineages 491714 2.7.11.24 mitogen-activated protein kinase cell culture insulin-treated rat 1 HIRc B cells 490760 2.7.11.24 mitogen-activated protein kinase cell culture MPK6 is associated with gamma-tubulin on microtubules in proliferating cells 741070 2.7.11.24 mitogen-activated protein kinase cell culture neoplasmic l(2)mbn cell line 491724 2.7.11.24 mitogen-activated protein kinase cell culture primary cell culture established from metacestode vesicle 690853 2.7.11.24 mitogen-activated protein kinase cell culture primary cultures prepared from embryonic mouse brains, i.e. cerebral hemisphere and thalamus 693778 2.7.11.26 tau-protein kinase cell culture HeLa cells 491683 2.7.11.26 tau-protein kinase cell culture primary midbrain mesencephalonic cells 662686 2.7.11.31 [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase cell culture - 693151 2.7.11.31 [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase cell culture primary hypothalamic or adipose cells 692267 2.7.11.7 myosin-heavy-chain kinase cell culture - -, 640779, 640780, 640781, 640782, 640783, 640784, 640790, 640793 2.7.11.7 myosin-heavy-chain kinase cell culture growth-phase cells 640776 2.7.11.7 myosin-heavy-chain kinase cell culture growth-phase cells contain enzyme form MHCK A 640777 2.7.11.7 myosin-heavy-chain kinase cell culture starved, developing cells 640778 2.7.11.7 myosin-heavy-chain kinase cell culture starved-developing cells contain enzyme form MHCK B 640777 2.7.11.9 Goodpasture-antigen-binding protein kinase cell culture HeLa cells 491668 2.7.12.1 dual-specificity kinase cell culture embryonal carcinoma cell line 532657 2.7.12.1 dual-specificity kinase cell culture most malignant tumors assessed express TTK mRNA, as well, all rapidly proliferating cell lines tested express TTK mRNA 532655 2.7.12.2 mitogen-activated protein kinase kinase cell culture from superior cervical ganglia 666126 2.7.13.3 histidine kinase cell culture optimal growth temperature for Synechocystis is 32-38°C, for both wild-type and DELTAHik34 mutant strains showing the same growth rates 740934 2.7.4.24 diphosphoinositol-pentakisphosphate 1-kinase cell culture - 662914 2.7.4.6 nucleoside-diphosphate kinase cell culture enzyme activity and protein level raise during exponential growth, no rise in activity when supply with sucrose is limited 676703 2.7.4.6 nucleoside-diphosphate kinase cell culture H50-7209 cell suspension culture 642648 2.7.6.1 ribose-phosphate diphosphokinase cell culture optimal growth temperature of Pyrobaculum calidifontis is between 90°C and 95°C -, 738194 2.7.7.12 UDP-glucose-hexose-1-phosphate uridylyltransferase cell culture derived from anterior pituitary gland gonadotropin-producing cells in different stages of the estrous cycle: proestrus, estrus, metestrus, and diestrus 642840 2.7.7.14 ethanolamine-phosphate cytidylyltransferase cell culture primary from brain cortical neurons 642888 2.7.7.35 ADP ribose phosphorylase cell culture - 643245, 643247 2.7.7.35 ADP ribose phosphorylase cell culture heterotrophically or autotrophically grown cells 643245, 643246, 643247 2.7.7.35 ADP ribose phosphorylase cell culture UV-mutant cells incapable of forming chloroplasts 643246 2.7.7.39 glycerol-3-phosphate cytidylyltransferase cell culture - -, 645241 2.7.7.4 sulfate adenylyltransferase cell culture - 738302 2.7.7.4 sulfate adenylyltransferase cell culture highest activity at the third day after inoculation, declining afterwards to a level found in resting cells -, 643274 2.7.7.4 sulfate adenylyltransferase cell culture maximal activity in 10-day old culture 643273 2.7.7.44 glucuronate-1-phosphate uridylyltransferase cell culture synchronous 645284 2.7.7.72 CCA tRNA nucleotidyltransferase cell culture optimal growth condition of the organismat 83°C 722982 2.7.7.84 2'-5' oligoadenylate synthase cell culture - 721471 2.7.7.84 2'-5' oligoadenylate synthase cell culture from spleen 721471 2.7.7.9 UTP-glucose-1-phosphate uridylyltransferase cell culture of hypertrophic and nonhypertrophic cells and chondrocytes 643756 2.7.7.9 UTP-glucose-1-phosphate uridylyltransferase cell culture optimization of pullulan production by evaluation of effects of controlled pH on fermentation time, pullulan production, biomass, and UDPG-pyrophosphorylase activity, overview. The activity of UDPG-pyrophosphorylase reaches a maximum at day 3 and decreases sharply after day 4 -, 721973 2.7.8.15 UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase cell culture - 645349, 645354 2.7.8.15 UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase cell culture the tunicamycin resistant cells have a greater than 40fold increase in the activity of the enzyme UDP-GlcNAc:dolichyl-P GlcNAc1P transferase. Increase in enzyme activity is due to an increased production of the enzyme 645356 2.7.8.24 phosphatidylcholine synthase cell culture - 394955, 394956 2.8.1.6 biotin synthase cell culture - 630 2.8.2.16 thiol sulfotransferase cell culture - 645744 2.8.2.20 protein-tyrosine sulfotransferase cell culture primary culture of umbilical vein endothelial cells, 2 isoforms: TPST1 and TPST2, shear stress causes a shift in protein expression from TPST1 to TPST2 645817 2.8.2.24 aromatic desulfoglucosinolate sulfotransferase cell culture derived from hypocotyls 643823, 643825 2.8.2.5 chondroitin 4-sulfotransferase cell culture chondrocytes 645750, 645751 2.8.2.5 chondroitin 4-sulfotransferase cell culture chondrosarcoma cell line 645916 2.8.2.8 [heparan sulfate]-glucosamine N-sulfotransferase cell culture CCL 44 cell 645938 2.8.2.8 [heparan sulfate]-glucosamine N-sulfotransferase cell culture CHO cell 645941 2.8.2.8 [heparan sulfate]-glucosamine N-sulfotransferase cell culture embryonic trachea 645938 2.8.3.5 3-oxoacid CoA-transferase cell culture - 645977 3.1.1.1 carboxylesterase cell culture - 750612 3.1.1.13 sterol esterase cell culture - 133856, 133858 3.1.1.20 tannase cell culture 9.97 U/mg production are obtained in 48 h by submerged fermentation in non-optimized condition. Maximal tannase production of 25.6 U/ml is obtained at 29.8°C with 12.7% tannic acid -, 752018 3.1.1.23 acylglycerol lipase cell culture - 666129 3.1.1.3 triacylglycerol lipase cell culture - -, 677635 3.1.1.3 triacylglycerol lipase cell culture batch or fed-batch culture in a medium containing citric acid and soybean oil as substrates 650654 3.1.1.3 triacylglycerol lipase cell culture enzyme production in presence of 1% olive oil or corn oil in the culture medium -, 650654 3.1.1.3 triacylglycerol lipase cell culture optimal and highest temperatures for growth of strain 7195 are 15°C and 35°C, respectively, exhibiting sigmoidal growth even at -3°C, no growth at 37°C or above 681355 3.1.1.3 triacylglycerol lipase cell culture optimal growth at pH 7.6 and 60°C, overview 677702 3.1.1.3 triacylglycerol lipase cell culture submerged batch culture 650654 3.1.1.32 phospholipase A1 cell culture fibroblast 666965 3.1.1.35 dihydrocoumarin hydrolase cell culture growth culture medium containing peptone and with the addition of sodium succinate, benzoic acid or 1-naphthol as the supplementary sole carbon source for induction of lactone-hydrolyzing enzyme activity -, 730943 3.1.1.74 cutinase cell culture - 691484 3.1.1.75 poly(3-hydroxybutyrate) depolymerase cell culture - -, 208334, 208335, 208336, 208339, 208340, 208341, 208342, 208343, 208346, 208347, 208348, 208349, 208350, 208351, 208352, 208353, 664693, 665369, 665380, 665392 3.1.1.77 acyloxyacyl hydrolase cell culture - 716472 3.1.1.78 polyneuridine-aldehyde esterase cell culture - 701693 3.1.11.1 exodeoxyribonuclease I cell culture - 133939, 133940, 133941, 133942, 133943, 133944, 133945, 133946, 133947, 133948, 133949, 133950, 133951, 133952 3.1.13.4 poly(A)-specific ribonuclease cell culture differentiated cells express PNLDC1 only after demethylation 751734 3.1.2.6 hydroxyacylglutathione hydrolase cell culture for RNA interference experiments a cell line (427 1313 514) is employed that encodes a second tetracycline repressor and constitutively expresses a T7-polymerase 694072 3.1.21.1 deoxyribonuclease I cell culture - 133978, 134202, 134205, 134232, 664029, 664587, 664665, 665170, 665370, 665545, 666736, 666780, 666887 3.1.21.7 deoxyribonuclease V cell culture - 288784, 288785, 288786, 288788 3.1.22.1 deoxyribonuclease II cell culture - 134280 3.1.26.11 tRNase Z cell culture - 664136, 664743, 665611, 665656, 665663, 666048, 666474, 666912, 677382 3.1.3.16 protein-serine/threonine phosphatase cell culture - 708027 3.1.3.2 acid phosphatase cell culture - 134738, 134742 3.1.3.2 acid phosphatase cell culture CCP-2, CCP-7 and CCP-8 cells from bone marrow 650794 3.1.3.2 acid phosphatase cell culture cell line XD-6 650393 3.1.3.2 acid phosphatase cell culture phosphate-starved 663928 3.1.3.26 4-phytase cell culture fermentation is optimized by a response surface methodology based on the Box-Behnken design, production leads to maximum activity of phytase of 205.45 U/ml -, 750979 3.1.3.26 4-phytase cell culture phytase increases markedly at the late stationary phase -, 653032 3.1.3.46 fructose-2,6-bisphosphate 2-phosphatase cell culture - 673215 3.1.3.5 5'-nucleotidase cell culture from submandibular salivary glands 664550 3.1.3.6 3'-nucleotidase cell culture - 95079 3.1.3.62 multiple inositol-polyphosphate phosphatase cell culture - 668231 3.1.3.69 glucosylglycerol 3-phosphatase cell culture the amount of stpA mRNA is increased by growth of the cells in the presence of NaCl concentratiins above 170 mM 170829 3.1.3.74 pyridoxal phosphatase cell culture - 667526, 668013, 669164 3.1.3.8 3-phytase cell culture fermentation is optimized by a response surface methodology based on the Box-Behnken design, production leads to maximum activity of phytase of 205.45 U/ml -, 750979 3.1.3.8 3-phytase cell culture phytase increases markedly at the late stationary phase -, 653032 3.1.4.4 phospholipase D cell culture - 135444 3.1.4.4 phospholipase D cell culture cells in suspension exhibit elevated levels of PLD activity compared to adherent cells, and as cells adhere to the substratum, PLD activity is downregulated 707550 3.1.4.4 phospholipase D cell culture from cv. Bright Yellow 2 710286 3.1.8.1 aryldialkylphosphatase cell culture an engineered strain growing on parathion -, 678836 3.13.2.1 adenosylhomocysteinase cell culture hypertonic stimulation induces association of S-adenosyl-L-homocysteine hydrolase with the F-actin-rich cell cortex. At intermediate, but not higher levels of hypertonicity, the enzyme further translocates from the cortex to the cytosol in company with a fraction of actin and cofilin. At the same time the cells round up. Acidification of the cells stimulates both the cell rounding and the translocation of S-adenosyl-L-homocysteine hydrolase and actin, whereas alkalinization retards these responses 668658 3.13.2.2 S-adenosyl-L-methionine hydrolase (L-homoserine-forming) cell culture - 668131, 670168 3.2.1.1 alpha-amylase cell culture isolated from a deep-sea hydrothermal vent -, 690582 3.2.1.14 chitinase cell culture culture condition suitable for production of chitinase are shaking at 30°C for 4 days in 100 ml of medium containing 1% shrimp and crab shell powder, 0.1% K2HPO4, and 0.05% MgSO4·7H2O, pH 7.0 -, 717981 3.2.1.14 chitinase cell culture maximal enzyme production at 37°C, pH 8. Maximal production at day 6 of culture -, 679004 3.2.1.14 chitinase cell culture the organism produces an extracellular chitinase when grown on the chitin as a sole carbon source -, 690645 3.2.1.143 poly(ADP-ribose) glycohydrolase cell culture of neuronal cell and astrocytes 666771 3.2.1.143 poly(ADP-ribose) glycohydrolase cell culture primary culture of astrocytes, neurons, and C6 glioma cells, no overall differences in enzyme activity between these cell types 664476 3.2.1.15 endo-polygalacturonase cell culture liquid culture 656714 3.2.1.162 lambda-carrageenase cell culture containing lambda-carrageenan as a carbon source 669161 3.2.1.209 endoplasmic reticulum Man9GlcNAc2 1,2-alpha-mannosidase cell culture - 135972 3.2.1.209 endoplasmic reticulum Man9GlcNAc2 1,2-alpha-mannosidase cell culture catalytically active soluble domain, released from endogenous proteases 135981 3.2.1.21 beta-glucosidase cell culture - -, 136306, 136321, 171306, 171589, 326275, 326277, 326279, 326281, 326282, 326283, 326286, 326287, 326288, 326290, 326293, 326294, 326295, 326303, 326304, 326305, 326307, 326310, 326311, 326313, 326314, 326319, 326320, 326322, 326325, 326326, 326329, 326330, 326332, 326333, 326334, 326335, 326339, 326342, 326343, 326344, 326346, 326347, 326348, 326352, 326355, 326357, 326358, 326363, 326365, 326367 3.2.1.21 beta-glucosidase cell culture 2 enzymes, beta-glucosidases I and II 326301 3.2.1.21 beta-glucosidase cell culture 2 forms, beta-glucosidase I and II 326354 3.2.1.21 beta-glucosidase cell culture 2 isoenzymes, PGI and PGII 326271 3.2.1.21 beta-glucosidase cell culture 3 different forms in the culture filtrate, 1 form intracellular 326324 3.2.1.21 beta-glucosidase cell culture 3 different forms, one with cellobiose-activity, two with exclusive aryl-beta-glucosidase activity 326369 3.2.1.21 beta-glucosidase cell culture 3 isoenzmes, A, B and C 326273 3.2.1.21 beta-glucosidase cell culture 3 isoforms, isoform Gb-1 most strongly induced by growth in cellulose medium 326364 3.2.1.21 beta-glucosidase cell culture 4 different isoforms 326360 3.2.1.21 beta-glucosidase cell culture five different forms in culture filtrate, 1 form cell-wall bound 326321 3.2.1.21 beta-glucosidase cell culture growth on 1.0% w/v carboxymethyl-cellulose 679908 3.2.1.21 beta-glucosidase cell culture maximal enzyme synthesis, if cells grown in wheat bran medium 326361, 326362 3.2.1.21 beta-glucosidase cell culture potent formation of cellobiase activity by induction by sophorose 326358 3.2.1.21 beta-glucosidase cell culture two forms, one membrane-bound, one extracellular 326328 3.2.1.23 beta-galactosidase cell culture - 171292, 171298, 171308 3.2.1.23 beta-galactosidase cell culture strain PaW85 (isogenic to fully sequenced KT2440) 692104 3.2.1.24 alpha-mannosidase cell culture - 135956, 135965, 135969, 135979, 135982, 135985, 135991, 136001, 136008, 136009, 136010, 136011, 136019, 586942 3.2.1.25 beta-mannosidase cell culture solid culture, with coffee waste and wheat bran -, 655292 3.2.1.26 beta-fructofuranosidase cell culture - -, 136099, 136112, 664178, 666543 3.2.1.26 beta-fructofuranosidase cell culture invertase F-form and invertase S-form 654834 3.2.1.28 alpha,alpha-trehalase cell culture activity in nitrogen-fixing pure culture is about 4times greater than in non-fixing cultures -, 136146 3.2.1.3 glucan 1,4-alpha-glucosidase cell culture - 136183, 679552 3.2.1.3 glucan 1,4-alpha-glucosidase cell culture cell culture growth conditions, optimization, partition factors, overview 682605 3.2.1.3 glucan 1,4-alpha-glucosidase cell culture optimal growth at pH 0.7 and 60 °C 679524 3.2.1.3 glucan 1,4-alpha-glucosidase cell culture solid culture on steamed rice 136184, 136205 3.2.1.3 glucan 1,4-alpha-glucosidase cell culture submerged fermentation conditions -, 750990 3.2.1.3 glucan 1,4-alpha-glucosidase cell culture submerged growth condition 679580 3.2.1.3 glucan 1,4-alpha-glucosidase cell culture wheat bran culture 136211 3.2.1.35 hyaluronoglucosaminidase cell culture - 171403, 171410 3.2.1.35 hyaluronoglucosaminidase cell culture derived from carcinoma cell lines 171395 3.2.1.36 hyaluronoglucuronidase cell culture - 171403, 171410 3.2.1.36 hyaluronoglucuronidase cell culture induction of enzyme synthesis by chondroitin sulfate, prevented by glucose, glycerol, lactate, pyruvate or succinate 171407 3.2.1.36 hyaluronoglucuronidase cell culture serological group B strains, 3402 type II and 4755 171399 3.2.1.37 xylan 1,4-beta-xylosidase cell culture - 136281, 136284, 136288, 136290, 136291, 136292, 136293, 136294, 136295, 136296, 136299, 136301, 136304, 136305, 136306, 136307, 136311, 136313, 136315 3.2.1.37 xylan 1,4-beta-xylosidase cell culture enzyme production induced in xylan or xylose media 136297, 136300, 136303 3.2.1.37 xylan 1,4-beta-xylosidase cell culture enzyme production most induced in xylan medium 136279 3.2.1.37 xylan 1,4-beta-xylosidase cell culture highest enzyme synthesis in sucrose/xylose medium 136283 3.2.1.37 xylan 1,4-beta-xylosidase cell culture induction of enzyme synthesis by xylose or xylan not required 136314 3.2.1.37 xylan 1,4-beta-xylosidase cell culture synthesis induced by methyl-beta- or methyl-alpha-D-xylopyranoside 136312 3.2.1.37 xylan 1,4-beta-xylosidase cell culture synthesis of enzyme only in xylose or methyl-beta-D-xylopyranoside media 136282 3.2.1.38 beta-D-fucosidase cell culture - 136317, 136321, 136324 3.2.1.39 glucan endo-1,3-beta-D-glucosidase cell culture - 136347, 136358, 136378 3.2.1.39 glucan endo-1,3-beta-D-glucosidase cell culture Eng2 is present during the mitotic cycle, and accumulates after meiosis II, expression of eng2+ is required for the efficient release of ascospores, expression of eng2+ is highly upregulated during the sporulation process, and the product is mainly involved in spore morphogenesis 708354 3.2.1.39 glucan endo-1,3-beta-D-glucosidase cell culture enzyme activation due to factors produced during conjugation and sporulation 136367 3.2.1.39 glucan endo-1,3-beta-D-glucosidase cell culture four different isoforms, beta-glucanases I, IIIB, IV and V 136362 3.2.1.39 glucan endo-1,3-beta-D-glucosidase cell culture from derepressed mycelia 136366 3.2.1.39 glucan endo-1,3-beta-D-glucosidase cell culture increasing activity with increasing culture age, maximum concentration reached at 7th day 136364 3.2.1.4 cellulase cell culture genes Tfu_1627, Tfu_1074, Tfu_2176, and Tfu_0901, encoding endo-1,4-beta-glucanases, expression analysis during stationary and exponential growth, overview 707254 3.2.1.4 cellulase cell culture growth of the strain SR22 marine isolate on CMC and sugarcane bagasse as sole carbon source, compared on solid media at different salinity ranges, overview 751310 3.2.1.4 cellulase cell culture metagenome 691346 3.2.1.40 alpha-L-rhamnosidase cell culture recombinant Echerichia coli cells carrying the rhmA gene of Thermomicrobia bacterium 679556 3.2.1.51 alpha-L-fucosidase cell culture - 136753, 208701, 208704, 208708, 208711, 208714, 208722, 208731 3.2.1.52 beta-N-acetylhexosaminidase cell culture cells in SE phase -, 709807 3.2.1.52 beta-N-acetylhexosaminidase cell culture liquid culture -, 655769 3.2.1.55 non-reducing end alpha-L-arabinofuranosidase cell culture optimization and evaluation of the culture conditions of alpha-L-arabinofuranosidase production by Talaromyces thermophilus, modelling, overview. Wheat bran is the best carbon source. Arabinose and arabitol are strong repressors of enzyme secretion. Except for sodium nitrate, all tested nitrogen sources stimulate alpha-L-arabinofuranosidase production, especially organic nitrogen sources, yeast extract gives the best yields 707245 3.2.1.58 glucan 1,3-beta-glucosidase cell culture growth on laminarin from Laminaria digitata or beta-glucan from the fruiting bodies of Pleurotus cornucopiae 710474 3.2.1.59 glucan endo-1,3-alpha-glucosidase cell culture expression of agn2+ is highly upregulated during the sporulation process, and the product is mainly involved in spore morphogenesis 708354 3.2.1.59 glucan endo-1,3-alpha-glucosidase cell culture submerged -, 649093 3.2.1.6 endo-1,3(4)-beta-glucanase cell culture Eng2 is present during the mitotic cycle, and accumulates after meiosis II, expression of eng2+ is required for the efficient release of ascospores, expression of eng2+ is highly upregulated during the sporulation process, and the product is mainly involved in spore morphogenesis 708354 3.2.1.67 galacturonan 1,4-alpha-galacturonidase cell culture - -, 666702, 668557, 669558, 671022, 671023 3.2.1.8 endo-1,4-beta-xylanase cell culture - 171611 3.2.1.8 endo-1,4-beta-xylanase cell culture endoxylanase production is optimized using agricultural bagasses and cost 20 times less than enzyme production using synthetic xylan -, 751636 3.2.1.84 glucan 1,3-alpha-glucosidase cell culture - 136906 3.2.1.85 6-phospho-beta-galactosidase cell culture activity is induced during growth on lactose but is absent in glucose-grown cells 677688 3.2.2.28 double-stranded uracil-DNA glycosylase cell culture poorly expressed in exponentially growing cells. Mug is overexpressed as cells enter stationary phase, and it is maintained at a fairly high level in resting cells. This is true of cells grown in rich or minimal media, and the principal regulation of mug is at the level of mRNA. Although the expression of mug is strongly dependent on the stationary-phase sigma factor, when cells are grown in minimal media, it shows only a modest dependence on stationary-phase sigma factor when cells are grown in rich media 694210 3.2.2.7 adenosine nucleosidase cell culture growth inhibition by 6-methylaminopurine 137108 3.2.2.7 adenosine nucleosidase cell culture rapid increase of activity following fungal elicitor treatment 137100 3.3.2.9 microsomal epoxide hydrolase cell culture numerous cancer cell lines and primary cells 663275 3.4.11.21 aspartyl aminopeptidase cell culture - 137170 3.4.11.6 aminopeptidase B cell culture - 36009 3.4.13.9 Xaa-Pro dipeptidase cell culture - 653207, 683216, 683612 3.4.13.9 Xaa-Pro dipeptidase cell culture growth condition study and role of dipeptides, proline- and glutamate-containing peptides may contribute to the adaptation of the organism to high salt through their intracellular hydrolysis and/or direct accumulation, overview 683559 3.4.14.1 dipeptidyl-peptidase I cell culture - 36151 3.4.14.1 dipeptidyl-peptidase I cell culture CD4(+) spleen cells 36151 3.4.14.1 dipeptidyl-peptidase I cell culture CD8(+) T cells 36151 3.4.14.10 tripeptidyl-peptidase II cell culture the extracellular enzyme is produced by the bacterium Streptomyces herbaricolor strain TY-21 during the late stationary growth phase -, 752530 3.4.15.1 peptidyl-dipeptidase A cell culture - 710024 3.4.17.14 Zinc D-Ala-D-Ala carboxypeptidase cell culture - 668822 3.4.17.18 carboxypeptidase T cell culture - 670772 3.4.17.21 Glutamate carboxypeptidase II cell culture - 668484 3.4.17.21 Glutamate carboxypeptidase II cell culture cerebral ischemia cell culture model 669776 3.4.17.21 Glutamate carboxypeptidase II cell culture cortical cells 656609 3.4.17.23 angiotensin-converting enzyme 2 cell culture - 708684, 710616 3.4.19.12 ubiquitinyl hydrolase 1 cell culture - 665803 3.4.21.107 peptidase Do cell culture mast cells 683885 3.4.21.115 infectious pancreatic necrosis birnavirus Vp4 peptidase cell culture - 667095, 669879, 670992 3.4.21.115 infectious pancreatic necrosis birnavirus Vp4 peptidase cell culture VP4 protease activity studied in the cell lines CHSE-214 and RSBK-2 that are susceptible to MABV infection, cell lines FHM and EPC resistant to MABV infection included as a control, infection of cell cultures with MABV at a multiplicity of infection of 0.1, respectively 683104 3.4.21.2 chymotrypsin C cell culture - 669441 3.4.21.35 tissue kallikrein cell culture primary, tracheal submucosal gland 647511 3.4.21.42 complement subcomponent C1s cell culture - 659718 3.4.21.57 Leucyl endopeptidase cell culture - 669818 3.4.21.68 t-Plasminogen activator cell culture in vitro model of the blood-brain barrier, primary rat astrocyte-enriched cultures are co-cultured with primary adult rat brain microvascular endothelial cells on opposite sides of a transwell membrane 710120 3.4.21.73 u-Plasminogen activator cell culture - 710248 3.4.21.83 Oligopeptidase B cell culture - 29784 3.4.21.83 Oligopeptidase B cell culture harvested at late log-phase 29788 3.4.21.94 proprotein convertase 2 cell culture - 709250 3.4.21.B12 prostase cell culture breast carcinoma cell line 647332 3.4.21.B30 UmuD protein cell culture - 669529 3.4.22.15 cathepsin L cell culture - 667305, 667428, 667863 3.4.22.38 cathepsin K cell culture - 700688 3.4.22.56 caspase-3 cell culture - 697570 3.4.22.56 caspase-3 cell culture priary neuronal cell culture derived from cerebral cortex of fetal rats 699679 3.4.22.B6 cathepsin B-like protease cell culture - 666453 3.4.23.18 Aspergillopepsin I cell culture submerged culture -, 30564 3.4.23.18 Aspergillopepsin I cell culture surface culture -, 30564, 30565 3.4.23.21 Rhizopuspepsin cell culture - 30627 3.4.23.45 memapsin 1 cell culture of muscle cells 638902 3.4.23.46 memapsin 2 cell culture transient acetylation and expression of BACE1 analyzed 683141 3.4.24.17 stromelysin 1 cell culture - 709370 3.4.24.35 gelatinase B cell culture glial-neuronal coculture 683874 3.4.24.40 serralysin cell culture optimization of cell culture medium ingredients for protease production, and of conditions: optimal at pH 8.0-9.0, 40°C, 0.2-0.4% Na2CO3, 0.1% phosphate, and under static conditions without agitating, overview -, 717656 3.4.24.40 serralysin cell culture optimization of fermentation production of a solvent stable thermoalkaline protease by a psychrotrophic Pseudomonas putida isolate, overview. Optimized to 1.0% glucose w/v, 2.0% gelatin, 0.5% yeast extract, and 0.01% w/v Mg2+ at pH 9.0, 25°C and 200 rpm, agitation and aeration processes are important variables, detailed overview -, 718079 3.4.24.40 serralysin cell culture optimization of protease production, optimal at pH 8.0, 27°C, with casein 717655 3.4.24.7 interstitial collagenase cell culture - 709370 3.4.24.80 membrane-type matrix metalloproteinase-1 cell culture neonatal cardiac myofibroblasts 683787 3.4.24.B4 matrix metalloproteinase-13 cell culture uterine smooth muscle cells 651954 3.5.1.1 asparaginase cell culture optimal conditions of induction are: inductor concentration of 0.1-0.2%, its addition during the middle of the logphase at 1.45-2.3 OU600, and culturing temperature of 28°C. The optimal duration of culturing after induction is 7-16 h -, 719200 3.5.1.1 asparaginase cell culture solid state, production evaluation and optimization of culture conditions using factorial designs -, 657205 3.5.1.105 chitin disaccharide deacetylase cell culture - -, 687337 3.5.1.121 protein N-terminal asparagine amidohydrolase cell culture - 740888 3.5.1.23 ceramidase cell culture 3-L culture 657260 3.5.1.24 choloylglycine hydrolase cell culture activity detected only in stationary-phase cells -, 209043 3.5.1.24 choloylglycine hydrolase cell culture optimization of culture conditions for the production of bile salt hydrolase, overview. Porduction is highest in sucrose- and peptone-grown cells 712058 3.5.1.26 N4-(beta-N-acetylglucosaminyl)-L-asparaginase cell culture - 209054 3.5.1.32 hippurate hydrolase cell culture - 172032, 172034 3.5.1.32 hippurate hydrolase cell culture induction of enzyme synthesis by hippurate-containing media 172033, 172035, 172036 3.5.1.35 D-glutaminase cell culture total activity remains stationary during ontogenesis 209084 3.5.1.38 glutamin-(asparagin-)ase cell culture - -, 209089, 209090, 209091, 209092, 209093, 209094, 209095, 209097, 209098, 209099, 209102 3.5.1.38 glutamin-(asparagin-)ase cell culture enzyme formation affected by culture conditions 209101 3.5.1.38 glutamin-(asparagin-)ase cell culture highest enzyme formation in L-glutamic acid containing medium 209103 3.5.1.38 glutamin-(asparagin-)ase cell culture induction of enzyme synthesis by addition of glutamic acid to the medium, glucose inhibits enzyme synthesis -, 209100 3.5.1.40 acylagmatine amidase cell culture - 172037, 172038 3.5.1.42 nicotinamide-nucleotide amidase cell culture - 171773, 209134, 209135, 209136, 209138, 209139, 209140, 209141 3.5.1.42 nicotinamide-nucleotide amidase cell culture including mutants lacking ability to hydrolyze nicotinamide D-ribonucleotide 209133 3.5.1.46 6-aminohexanoate-oligomer exohydrolase cell culture - -, 172039, 172042, 172043 3.5.1.46 6-aminohexanoate-oligomer exohydrolase cell culture nylB gene, enzyme EII -, 172040, 172041 3.5.1.46 6-aminohexanoate-oligomer exohydrolase cell culture nylB' gene, enzyme EII' -, 172040, 172041 3.5.1.47 N-acetyldiaminopimelate deacetylase cell culture - 172044, 172045 3.5.1.48 acetylspermidine deacetylase cell culture - 172047, 172048 3.5.1.49 formamidase cell culture - -, 209143, 209144, 209148, 209149 3.5.1.49 formamidase cell culture enhanced activity during dual CN-limited growth conditions 209142 3.5.1.49 formamidase cell culture induction of enzyme synthesis using formamide- or acetamide-containing media -, 209145, 209146 3.5.1.49 formamidase cell culture only detectable in fast growing strains 209147 3.5.1.75 urethanase cell culture 80% of urethanase activity is cell associated 209239 3.5.1.75 urethanase cell culture urethanase activity detected in the cells not in the culture medium 209236, 209237 3.5.1.88 peptide deformylase cell culture - 393799, 393806, 393807, 393811 3.5.1.88 peptide deformylase cell culture increasing activity by addition of Ni2+ or Mn2+ ions to the culture medium 393803 3.5.1.9 arylformamidase cell culture - 209249, 209252, 209254, 209261 3.5.1.9 arylformamidase cell culture decreasing formamidase synthesis in presence of anthranilic acid, p-aminobenzoic acid, 3-hydroxyanthranilic acid, kynurenine or 3-hydroxykynurenine 209248 3.5.2.6 beta-lactamase cell culture - 693359 3.5.2.6 beta-lactamase cell culture strain DU25151/00 692632 3.5.5.1 nitrilase cell culture - -, 209747, 209748, 209749, 209754, 209759 3.5.5.1 nitrilase cell culture best inducer of enzyme formation: benzonitrile 209745, 209746 3.5.5.1 nitrilase cell culture best inducer of enzyme formation: isobutyronitrile 209755 3.5.5.1 nitrilase cell culture induction of enzyme synthesis by epsilon-caprolactam- or isovaleronitrile-containing media 209743 3.5.5.1 nitrilase cell culture optimization of culture conditions for nitrilase production, overview. Fe2+ is the best metal ion, pH 7.0 and 28°Care the best conditions, phenylacetamide is the best inducer -, 718611 3.5.5.1 nitrilase cell culture optimization of enzyme production, best conditions are glucose 1.0%, acrylonitrile 0.1%, pH 7.0 -, 719103 3.5.5.1 nitrilase cell culture use of benzonitrile as a sole source of nitrogen and carbon -, 209758, 209761, 209762, 209763 3.5.5.1 nitrilase cell culture using bromoxynil as a sole source of nitrogen 209756, 209757 3.5.99.7 1-aminocyclopropane-1-carboxylate deaminase cell culture - 693861 3.5.99.7 1-aminocyclopropane-1-carboxylate deaminase cell culture four different morphotypes of biovar B, strain HS-2 and the strain UW4 as control strain 691950 3.5.99.7 1-aminocyclopropane-1-carboxylate deaminase cell culture strain UW4 -, 694780 3.5.99.7 1-aminocyclopropane-1-carboxylate deaminase cell culture strain UW4 AcdS+ (ability to synthesize ACC deaminase and indole acetic acid) and its mutant strain AcdS- -, 692374 3.6.1.6 nucleoside diphosphate phosphatase cell culture - 656961 3.6.1.9 nucleotide diphosphatase cell culture - 246818, 246828, 246832 3.6.1.9 nucleotide diphosphatase cell culture growth inhibition by AMP, ADP when NAD serves as V-factor 246814 3.6.1.9 nucleotide diphosphatase cell culture potential growth inhibition by adenosine diphosphate 246809 3.6.5.2 small monomeric GTPase cell culture - 644131 3.6.5.2 small monomeric GTPase cell culture implicated in regulation of phagocytosis 644114 3.7.1.18 6-oxocamphor hydrolase cell culture strain NCIMB 9784 is grown on (1R)-(+)-camphor as the sole carbon source -, 719804 3.7.1.7 beta-diketone hydrolase cell culture batch cultivation of a mixed culture at different pH values, Pseudomonas sp. OPH is most active, 2.11 U/ml, and stable at pH values between pH 6.0 and pH 7.0, highest production rate at pH 7.0 with 44.2 U/g/h, two-stage pH control strategy, overview -, 720212 3.7.1.7 beta-diketone hydrolase cell culture batch cultivation of a mixed culture at different pH values, Pseudomonas sp. OPH is most active, 2.11 U/ml, and stable at pH values between pH 6.0 and pH 7.0, two-stage pH control strategy, overview -, 720212 3.8.1.3 haloacetate dehalogenase cell culture growth with inducing substrates 3-chlorobutyric sodium, and 3-hydroxybutyric sodium, 3-chlorobutyrate, and 3-hydroxybutyrate -, 719141 3.8.1.7 4-chlorobenzoyl-CoA dehalogenase cell culture strain P25 utilizes 4-chlorobiphenyl and 4-chlorobenzoic acid as sole carbon and energy sources -, 720401 4.1.1.14 valine decarboxylase cell culture highest activity in very young cultures, decreases rapidly in the early exponential phase of growth 3969 4.1.1.15 glutamate decarboxylase cell culture - 691484 4.1.1.17 ornithine decarboxylase cell culture embryogenic cultures 746678 4.1.1.17 ornithine decarboxylase cell culture maximal activity in the middle and late log phases of growth 4007 4.1.1.18 lysine decarboxylase cell culture - 37284 4.1.1.18 lysine decarboxylase cell culture drastic descent of enzyme activity owing to degradation of LDC at entry into the stationary phase of cell growth 654982 4.1.1.18 lysine decarboxylase cell culture grown in chemically defined medium without Lys 37290 4.1.1.19 arginine decarboxylase cell culture - 4088 4.1.1.20 diaminopimelate decarboxylase cell culture - 2153 4.1.1.25 tyrosine decarboxylase cell culture - 4194, 4195, 4196, 4197 4.1.1.32 phosphoenolpyruvate carboxykinase (GTP) cell culture grown autotrophically with CO2 in the light. The yield is higher when an additional carbon source like glucose is added. Highest yields result if both glucose and CO2 are provided together in the dark. No activity in cells provided with CO2 alone and kept in the dark -, 4355 4.1.1.36 phosphopantothenoylcysteine decarboxylase cell culture activity reaches maximal levels in the early stationary phase 4412 4.1.1.4 acetoacetate decarboxylase cell culture - -, 4523 4.1.1.4 acetoacetate decarboxylase cell culture resting 4538 4.1.1.49 phosphoenolpyruvate carboxykinase (ATP) cell culture - 694645 4.1.1.65 phosphatidylserine decarboxylase cell culture chemoheterotrophically and phototrophically grown 4778 4.1.1.7 benzoylformate decarboxylase cell culture - 691346 4.1.1.78 acetylenedicarboxylate decarboxylase cell culture - 648381 4.1.1.79 sulfopyruvate decarboxylase cell culture ISM strain 698626 4.1.3.36 1,4-dihydroxy-2-naphthoyl-CoA synthase cell culture - 93959 4.1.99.2 tyrosine phenol-lyase cell culture wild-type S12 and its mutant strains S12TPL3 (optimized for phenol production) 692854 4.1.99.3 deoxyribodipyrimidine photo-lyase cell culture - 33494 4.1.99.3 deoxyribodipyrimidine photo-lyase cell culture confluent murine cells have higher specific activity than do rapidly growing cells 33494 4.2.1.103 cyclohexyl-isocyanide hydratase cell culture the enzyme is expressed in cells growing on isonitrile as the sole carbon and nitrogen sources, highest enzyme activity is strain F164 in a nutrient medium containing N-benzylformamide. No induction by growth on glycerol and (NH4)2SO4 as the sole carbon and nitrogen sources, respectively -, 715552 4.2.1.105 2-hydroxyisoflavanone dehydratase cell culture - 656937 4.2.1.108 ectoine synthase cell culture photosynthetically grown cells 640090 4.2.1.118 3-dehydroshikimate dehydratase cell culture cultured in presence of shikimate 700722 4.2.1.121 colneleate synthase cell culture preferential stimulation of the 9-lipoxygenase pathway in elicitor-treated potato cells 704400 4.2.1.125 dammarenediol II synthase cell culture hairy root culture 683467 4.2.1.143 versicolorin B synthase cell culture highest abundance of enzyme in colony fraction S2 consisting of cells that have grown for 24-48 h 721453 4.2.1.18 methylglutaconyl-CoA hydratase cell culture - 33740 4.2.1.78 (S)-norcoclaurine synthase cell culture - 5894, 5895, 716518 4.2.2.10 pectin lyase cell culture in medium containing sodium polypectate or cell walls from Phaseolus vulgaris 34003 4.2.2.7 heparin lyase cell culture optimization of enzyme production, simple carbon sources, including sucrose, lactose, maltose, and complex carbon sources such as starch and cellulose are able to support growth of the organism, but enzyme can be produced only in the presence of maltose, sorbitol, mannitol, arabinose, and mannose. Phosphate inhibits enzyme production and cell growth, while heparin inhibits cell growth but is required for enzyme induction, overview. Heparinase production in optimized medium is highest, i.e., 75.4 units, when the pH of the medium is kept at pH 6.5, inoculum size of 0.5-1.0% of spore suspension, and for an incubation temperature of 30°C. Growth profile, heparin utilization, and heparinase production in optimized medium, overview 693981 4.2.3.1 threonine synthase cell culture - 137558, 137562 4.2.3.12 6-pyruvoyltetrahydropterin synthase cell culture the starting cells, which are harvested just after plating on the developmental plate exhibit high PTPS mRNA. After 4 h, the transcripts decrease dramatically and then remain at the levels thereafter, although increases slightly at the terminal stage (after 12 h) -, 667828 4.2.3.17 taxadiene synthase cell culture - 693993, 694301 4.2.3.18 abieta-7,13-diene synthase cell culture - 694607 4.2.3.20 (R)-limonene synthase cell culture - 691407 4.2.3.22 germacradienol synthase cell culture strain ATCC 27952 produces clinically important anthracycline chemotherapeutic agents of the polyketide class of antibiotics, daunorubicin and doxorubicin 693594 4.2.3.24 amorpha-4,11-diene synthase cell culture - 693474 4.2.3.27 isoprene synthase cell culture from leaves 668607 4.2.3.5 chorismate synthase cell culture - 691478, 692285 4.2.3.7 pentalenene synthase cell culture from a 60 h cell culture -, 648596 4.2.3.93 delta-guaiene synthase cell culture - 716607 4.2.99.18 DNA-(apurinic or apyrimidinic site) lyase cell culture - 648620, 692875, 747046, 747210 4.3.1.1 aspartate ammonia-lyase cell culture - 691570, 694045 4.3.1.1 aspartate ammonia-lyase cell culture strain KIM, possesses a base transversion (GC-TA) at position 146 of AspA, causing replacement of aspartate by tyrosine 694029 4.3.1.1 aspartate ammonia-lyase cell culture strain NCTC 11168 694233 4.3.1.1 aspartate ammonia-lyase cell culture strain PB1 of serogroup 1b 694029 4.3.1.12 ornithine cyclodeaminase cell culture lysates of parasite culture isolates FF28JT-Rio (low virulent) and FMV-1 (highly virulent) 693873 4.3.1.24 phenylalanine ammonia-lyase cell culture - 34329, 34340, 34342, 34357, 34358 4.3.1.24 phenylalanine ammonia-lyase cell culture kinetin-treated and untreated cells 34336 4.3.3.2 strictosidine synthase cell culture - 648713, 648714, 648715, 648716, 648717, 648718, 648719, 648720, 648721, 648723, 648725, 648729 4.3.3.2 strictosidine synthase cell culture line Utr-3a 729484 4.4.1.1 cystathionine gamma-lyase cell culture whole cells, cell lysate of a culture broth, cell wall of a subcellular fraction 691353 4.4.1.13 cysteine-S-conjugate beta-lyase cell culture - -, 34710, 715070 4.4.1.15 D-cysteine desulfhydrase cell culture - 648735 4.4.1.25 L-cysteate sulfo-lyase cell culture ISM strain 698626 4.6.1.1 adenylate cyclase cell culture of isolated ventricular myocytes 693847 4.6.1.19 ribonuclease T2 cell culture - 656479, 656707 5.1.1.3 glutamate racemase cell culture highest activity in the early stationary phase of growth -, 2108 5.2.1.8 peptidylprolyl isomerase cell culture carbon-starved cells 701755 5.2.1.8 peptidylprolyl isomerase cell culture primary cortical culture 693136 5.3.1.5 xylose isomerase cell culture Pseudomonas strain S12 containing xylose isomerase gene and xylulokinase gene from Escherichia coli strain DH5 alpha 690541 5.3.2.6 2-hydroxymuconate tautomerase cell culture strain JJ-1b is able to grow on 4-hydroxybenzoate as a sole source of carbon and energy 704310 5.3.3.1 steroid DELTA-isomerase cell culture - 693273 5.3.3.1 steroid DELTA-isomerase cell culture biotype B 692914 5.3.3.12 L-dopachrome isomerase cell culture - 704760 5.3.3.12 L-dopachrome isomerase cell culture preparation of single-cell suspensions of the tail skin -, 703809 5.3.99.7 styrene-oxide isomerase cell culture cells grown in a styrene gas atmosphere -, 648825 5.4.2.10 phosphoglucosamine mutase cell culture - 210870, 210871, 210874 5.4.4.2 isochorismate synthase cell culture - 492275, 492276, 492277 5.4.99.15 (1->4)-alpha-D-glucan 1-alpha-D-glucosylmutase cell culture 10fold increase of enzyme activity in soybean nodules infected with Bradyrhizobium japonicum compared to bacterial culture 677667 5.4.99.15 (1->4)-alpha-D-glucan 1-alpha-D-glucosylmutase cell culture 8fold increase of enzyme activity in soybean nodules infected with Bradyrhizobium elkanii compared to bacterial culture 677667 5.4.99.16 maltose alpha-D-glucosyltransferase cell culture analysis of enzyme activity at cell growth under pressure of 0.1-1.5 MPa and for 0.5-3 h, overview. For highest enzyme activity, the bacteria are cultured at 70°C, 150 rpm, at 1 MPa for 2 h, in a medium containing 30 g/l potassium glutamate -, 749423 5.4.99.41 lupeol synthase cell culture - 730620 5.4.99.7 Lanosterol synthase cell culture - 3591, 3597 5.4.99.8 cycloartenol synthase cell culture - 748718 5.5.1.12 copalyl diphosphate synthase cell culture - 694607 5.5.1.6 chalcone isomerase cell culture - 3747, 603 6.1.1.2 tryptophan-tRNA ligase cell culture peripheral blood mononuclear cells 745868 6.2.1.12 4-coumarate-CoA ligase cell culture - 602, 603, 606, 608, 611, 728204 6.2.1.13 acetate-CoA ligase (ADP-forming) cell culture - 649507, 651644 6.2.1.14 6-carboxyhexanoate-CoA ligase cell culture - -, 630, 649669 6.2.1.30 phenylacetate-CoA ligase cell culture maximal activity in mid-exponential growth pase -, 648916 6.2.1.B1 O-succinylbenzoate-CoA ligase (ADP-forming) cell culture - 649038 6.3.1.2 glutamine synthetase cell culture a much larger amount of GlnA1 is produced than is necessary for normal growth by the organism and a substantial proportion of this is found in the extracellular medium of cell cultures -, 692709 6.3.1.2 glutamine synthetase cell culture leaf protoplast derived suspension culture 657078 6.3.2.11 carnosine synthase cell culture primary kidney cells 744004 6.3.2.2 glutamate-cysteine ligase cell culture - 651391, 651392 6.3.2.2 glutamate-cysteine ligase cell culture astrocyte, primary 649315 6.3.2.2 glutamate-cysteine ligase cell culture optimal fermentation conditions are 25°C, 2 days, pH 6.0, and aeration level 1 vvm -, 745888 6.3.2.3 glutathione synthase cell culture Chang cell 650330 6.3.2.3 glutathione synthase cell culture hepatocyte 650330 6.3.2.3 glutathione synthase cell culture optimal fermentation conditions are 25°C, 2 days, pH 6.0, and aeration level 1 vvm -, 745888 6.3.5.4 asparagine synthase (glutamine-hydrolysing) cell culture - 726824 6.3.5.4 asparagine synthase (glutamine-hydrolysing) cell culture SB-P, dark-adapted 650596 6.3.5.5 carbamoyl-phosphate synthase (glutamine-hydrolysing) cell culture growth medium is bovine milk 704229 6.5.1.1 DNA ligase (ATP) cell culture LIG k alpha localizes to the kinetoplast primarily in cells that have completed mitosis and contain either a dividing kinetoplast or two newly divided kinetoplasts. The ligase is present on both faces of the kDNA disk and at a high level in the kinetoflagellar zone of the mitochondrial matrix. LIG k alpha transcript levels are maximal during the phase when cells contain two nuclei 661615 7.1.1.9 cytochrome-c oxidase cell culture primary cardiac cell culture of neonatal rats, increase in enzyme activity by interaction of enzyme subunit IV with protein kinase Cepsilon upon hypoxia 671844 7.1.2.2 H+-transporting two-sector ATPase cell culture - 720031 7.1.2.2 H+-transporting two-sector ATPase cell culture Enterococcus hirae grows well under anaerobic conditions at alkaline pH 8.0 -, 711693 7.6.2.11 ABC-type polyamine transporter cell culture Escherichia coli can grow on putrescine as a sole carbon or nitrogen source -, 751075