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Literature summary extracted from

  • Shin, D.; Park, C.
    N-terminal extension of canine glutamine synthetase created by splicing alters its enzymatic property (2004), J. Biol. Chem., 279, 1184-1190.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.1.2 short and long isoform expressed in Escherichia coli BL21(DE3) Canis lupus familiaris

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.1.2 L-methionine-S-sulfoximine
-
 Canis lupus familiaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.1.2 1.1
-
 L-glutamate short isoform, pH 7.2, 37°C Canis lupus familiaris
6.3.1.2 1.3
-
 L-glutamate long isoform, pH 7.2, 37°C Canis lupus familiaris
6.3.1.2 1.3
-
 ATP short isoform, pH 7.2, 37°C Canis lupus familiaris
6.3.1.2 1.9
-
 ATP long isoform, pH 7.2, 37°C Canis lupus familiaris

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.3.1.2 cytosol
-
 Canis lupus familiaris 5829
-
6.3.1.2 membrane
-
 Canis lupus familiaris 16020
-
6.3.1.2 nucleus low activity Canis lupus familiaris 5634
-
6.3.1.2 particle-bound low activity Canis lupus familiaris
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.1.2 45000
-
 SDS-PAGE, short isoform Canis lupus familiaris
6.3.1.2 49000
-
 SDS-PAGE, long isoform Canis lupus familiaris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.1.2 ATP + glutamate + NH4+ Mus musculus central enzyme of nitrogen metabolism, elimination of glutamate from animal brain ADP + phosphate + L-glutamine
-
 ?
6.3.1.2 ATP + glutamate + NH4+ Homo sapiens central enzyme of nitrogen metabolism, elimination of glutamate from animal brain ADP + phosphate + L-glutamine
-
 ?
6.3.1.2 ATP + glutamate + NH4+ Canis lupus familiaris central enzyme of nitrogen metabolism, elimination of glutamate from animal brain ADP + phosphate + L-glutamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.2 Canis lupus familiaris
-
-
-
6.3.1.2 Canis lupus familiaris
-
 two isoforms generated by alternative splicing, differences in molecular weights
-
6.3.1.2 Homo sapiens
-
-
-
6.3.1.2 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.1.2 recombinant proteins using His-tag Canis lupus familiaris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.1.2 brain short and long isoform Canis lupus familiaris
-
6.3.1.2 liver short and long isoform Canis lupus familiaris
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.2 ATP + glutamate + NH4+
-
 Mus musculus ADP + phosphate + L-glutamine
-
 ?
6.3.1.2 ATP + glutamate + NH4+
-
 Homo sapiens ADP + phosphate + L-glutamine
-
 ?
6.3.1.2 ATP + glutamate + NH4+
-
 Canis lupus familiaris ADP + phosphate + L-glutamine
-
 ?
6.3.1.2 ATP + glutamate + NH4+ central enzyme of nitrogen metabolism, elimination of glutamate from animal brain Mus musculus ADP + phosphate + L-glutamine
-
 ?
6.3.1.2 ATP + glutamate + NH4+ central enzyme of nitrogen metabolism, elimination of glutamate from animal brain Homo sapiens ADP + phosphate + L-glutamine
-
 ?
6.3.1.2 ATP + glutamate + NH4+ central enzyme of nitrogen metabolism, elimination of glutamate from animal brain Canis lupus familiaris ADP + phosphate + L-glutamine
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.3.1.2 0.067
-
 L-methionine-S-sulfoximine short isoform, pH 7.2, 37°C Canis lupus familiaris
6.3.1.2 0.124
-
 L-methionine-S-sulfoximine long isoform, pH 7.2, 37°C Canis lupus familiaris