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Literature summary extracted from

  • Shatters, R.G.; Liu, Y.; Kahn, M.L.
    Isolation and characterization of a novel glutamine synthetase from Rhizobium meliloti (1993), J. Biol. Chem., 268, 469-475.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.1.2 ADP enzyme form GSIII Sinorhizobium meliloti
6.3.1.2 diphosphate enzyme form GSIII Sinorhizobium meliloti
6.3.1.2 L-methionine sulfoximine the concentration needed to inhibit GSIII is 50-100times higher than that needed to inhibit GSI or GSII Sinorhizobium meliloti

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.1.2 5.3
-
hydroxylamine enzyme form GSIII Sinorhizobium meliloti
6.3.1.2 13.3
-
Glu enzyme form GSIII Sinorhizobium meliloti
6.3.1.2 33
-
NH4+ enzyme form GSIII Sinorhizobium meliloti

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.1.2 46500
-
x * 46500 + x * 49000, SDS-PAGE Sinorhizobium meliloti
6.3.1.2 49000
-
x * 46500 + x * 49000, SDS-PAGE Sinorhizobium meliloti
6.3.1.2 355000
-
gel filtration Sinorhizobium meliloti

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.2 Sinorhizobium meliloti
-
3 enzyme forms: GSI, GSII and GSIII
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.1.2 GSIII Sinorhizobium meliloti

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.2 ATP + L-Glu + NH4+
-
Sinorhizobium meliloti ADP + phosphate + L-Gln
-
?
6.3.1.2 ATP + L-glutamate + hydroxylamine
-
Sinorhizobium meliloti ADP + phosphate + gamma-glutamyl hydroxamate
-
?

Subunits

EC Number Subunits Comment Organism
6.3.1.2 octamer x * 46500 + x * 49000, SDS-PAGE Sinorhizobium meliloti

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.1.2 50
-
enzyme form GSIII Sinorhizobium meliloti

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.1.2 6.8
-
enzyme form GSIII Sinorhizobium meliloti